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Human Galectin-3 Antibody
R&D Systems, Inc. a Bio-Techne Brand
Catalog # MAB11541
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Key Product Details
| Species Reactivity: | Human |
| Immunogen: |
E. coli-derived recombinant human Galectin‑3 Ala2-Ile250 Accession # P17931.5 |
| Applications: | ELISA Capture, Immunohistochemistry, Simple Western, Western Blot |
| Label: | Unconjugated |
| Source: | Monoclonal Mouse IgG2B Clone # 194805 |
| Specificity: |
Detects human Galectin-3 in ELISAs. In sandwich immunoassays, no cross-reactivity with recombinant human Galectin-1, 2, 4, 7, 8, or 10 is observed. |
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Western BlotDetection of Human Galectin‑3 by Western Blot. Western blot shows lysates of COLO 205 human colorectal adenocarcinoma cell line, MCF-7 human breast cancer cell line, and U-118-MG human glioblastoma/astrocytoma cell line. PVDF membrane was probed with 0.2 µg/mL of Mouse Anti-Human Galectin-3 Monoclonal Antibody (Catalog # MAB11541) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Galectin-3 at approximately 28 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1. |
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ImmunohistochemistryGalectin‑3 in Human Prostate Cancer Tissue. Galectin-3 was detected in immersion fixed paraffin-embedded sections of human prostate cancer tissue using Mouse Anti-Human Galectin-3 Monoclonal Antibody (Catalog # MAB11541) at 1.7 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm and plasma membrane in epithelial cells. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents. |
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Simple WesternDetection of Human Galectin‑3 by Simple WesternTM. Simple Western lane view shows lysates of COLO 205 human colorectal adenocarcinoma cell line, MCF‑7 human breast cancer cell line, and U‑118‑MG human glioblastoma/astrocytoma cell line, loaded at 0.2 mg/mL. A specific band was detected for Galectin‑3 at approximately 38 kDa (as indicated) using 10 µg/mL of Mouse Anti-Human Galectin‑3 Monoclonal Antibody (Catalog # MAB11541). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system. Non-specific interaction with the 230 kDa Simple Western standard may be seen with this antibody. |
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Customer Reviews for Human Galectin-3 Antibody (4)
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Application: WBSample Tested: Cell lysate from colon cancer cell lineVerified Customer | Posted 10/26/2015Very sensitive, followed Milk block, primary BSA, Secondary Milk. As low as 1:2500 works too. Specificity: SpecificSensitivity: SensitiveBuffer: Milk, BSA, MilkDilution: 1:1000
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Application: IHCSample Tested: Cancer TissueSpecies: HumanVerified Customer | Posted 10/26/2015The antibody is specific and detects only cells but not the background. Specificity: SpecificSensitivity: SensitiveBuffer: PBSDilution: 1:1000
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Application: ICC/IFSample Tested: Colon cancer cell lineSpecies: HumanVerified Customer | Posted 10/26/2015Very good binding ability. Detects galectin-3 at low dilutions also. Specificity: Reasonably specificSensitivity: SensitiveBuffer: PBSDilution: 1:1000
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Application: FlowSample Tested: Human cell lineSpecies: HumanVerified Customer | Posted 10/26/2015Galectin-3 is detectable on the surface of the cells and is suitable at lower dilutions also. Specificity: SpecificSensitivity: SensitiveBuffer: PBSDilution: 1:1000
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