Skip to main content

Novus Biologicals products are now on bio-techne.com

Better as one

Human G‑CSF Antibody

Bio-Techne includes R&D Systems | Catalog # AF-214-NA

Catalog #
Availability
Size / Price
Qty
Loading...
AF-214-NA
AF-214-SP

Key Product Details

Species Reactivity

Human

Applications

Neutralization, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Product Summary for Human G‑CSF Antibody

Immunogen

E. coli-derived recombinant human G-CSF
Thr31-Pro204
Accession # NP_757373

Specificity

Detects human G-CSF in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 30% cross-reactivity with recombinant mouse G-CSF and recombinant canine G-CSF is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human G‑CSF Antibody

Detection of Recombinant Human G-CSF antibody by Western Blot.

Detection of Recombinant Human G‑CSF by Western Blot.

Western blot shows 25 ng of Recombinant Human G-CSF (Catalog # 214-CS) and Recombinant Mouse G-CSF (Catalog # 414-CS). PVDF Membrane was probed with 0.1 µg/mL of Goat Anti-Human G-CSF Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-214-NA) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for G-CSF at approximately 18 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3.
Cell Proliferation Induced by G‑CSF and Neutralization by Human G‑CSF Antibody.

Cell Proliferation Induced by G‑CSF and Neutralization by Human G‑CSF Antibody.

Recombinant Human G-CSF (Catalog # 214-CS) stimulates proliferation in the NFS-60 mouse myeloid cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human G-CSF (0.125 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human G-CSF Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-214-NA). The ND50 is typically 8-48 ng/mL.

Applications for Human G‑CSF Antibody

Application
Recommended Usage

Western Blot

0.1 µg/mL
Sample: Recombinant Human G-CSF (Catalog # 214-CS)

Neutralization

Measured by its ability to neutralize G‑CSF-induced proliferation in the M‑NFS‑60 mouse myeloid cell line. Shirafuji, N.et al. (1989) Exp. Hematol. 17:116. The Neutralization Dose (ND50) is typically 8-48 ng/mL in the presence of 0.125 ng/mL Recombinant Human G‑CSF.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 1 review rated 4 using AF-214-NA in the following applications:

Published Applications

Read 6 publications using AF-214-NA in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
Size / Price
Qty
Loading...

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: G-CSF

G-CSF is a pleiotropic cytokine best known for its specific effects on the proliferation, differentiation, and activation of hematopoietic cells of the neutrophilic granulocyte lineage. It is produced mainly by monocytes and macrophages upon activation by endotoxin, TNF-alpha and IFN-gamma. Other cell types including fibroblasts, endothelial cells, astrocytes and bone marrow stromal cells can also secrete G-CSF after LPS, IL-1, or TNF-alpha activation. In addition, various carcinoma cell lines and myeloblastic leukemia cells can express G-CSF constitutively.

In humans, two distinct cDNA clones for G-CSF, encoding 207 and 204 amino acid precursor proteins, have been isolated. Both proteins have a 30 amino acid signal peptide and have identical amino acid sequences except for a three amino acid insertion (deletion) at the 35th amino acid residue from the N-terminus of the mature protein. Human G-CSF is 73% identical at the amino acid level to murine G-CSF and the two proteins show species cross-reactivity.

In vitro, G-CSF stimulates growth, differentiation and functions of cells from the neutrophil lineage. It also has blast cell growth factor activity and can synergize with IL-3 to shorten the Go period of early hematopoietic progenitors. Consistent with its in vitro functions, G-CSF has been found to play important roles in defense against infection, in inflammation and repair, and in the maintenance of steady state hematopoiesis.

Long Name

Granulocyte Colony Stimulating Factor

Alternate Names

C17orf33, CSF3, CSF3OS, Filgrastim, GCSF, Lenograstim, Pluripoietin

Entrez Gene IDs

1440 (Human); 12985 (Mouse)

Gene Symbol

CSF3

UniProt

Additional G-CSF Products

Product Documents for Human G‑CSF Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human G‑CSF Antibody

For research use only

Loading...
Loading...

Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

÷
=