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Human G‑CSF Antibody

Bio-Techne includes R&D Systems | Catalog # MAB214

Catalog #
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MAB214-100
MAB214-500
MAB214-SP

Key Product Details

Species Reactivity

Human

Applications

ELISA Capture (Matched Antibody Pair), Neutralization

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 3316

Product Summary for Human G‑CSF Antibody

Immunogen

E. coli-derived recombinant human G-CSF
Ala30-Pro204
Accession # NP_757373

Specificity

Captures human G-CSF in sandwich ELISAs. In this format, no cross-reactivity or interference was observed with recombinant human(rh) GM-CSF, rmGM-CSF, rhM-CSF, or rmM-CSF. This antibody cross-reacts with recombinant mouse G-CSF at concentrations greater than 50 ng/mL.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human G‑CSF Antibody

Cell Proliferation Induced by G‑CSF and Neutralization by Human G‑CSF Antibody.

Cell Proliferation Induced by G‑CSF and Neutralization by Human G‑CSF Antibody.

Recombinant Human G-CSF (Catalog # 214-CS) stimulates proliferation in the NFS-60 mouse myeloid cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human G-CSF (0.125 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human G-CSF Monoclonal Antibody (Catalog # MAB214). The ND50 is typically 0.01-0.03 µg/mL.

Applications for Human G‑CSF Antibody

Application
Recommended Usage

Neutralization

Measured by its ability to neutralize G‑CSF-induced proliferation in the NFS‑60 mouse myeloid cell line. Shirafuji, N.et al. (1989) Exp. Hematol. 17:116. The Neutralization Dose (ND50) is typically 0.01-0.03 µg/mL in the presence of 0.125 ng/mL Recombinant Human G‑CSF.

Human G-CSF Sandwich Immunoassay

ELISA Capture (Matched Antibody Pair)
Recommended Concentration: 2-8 µg/mL
Use in combination with these reagents:
  • Detection Reagent: Human G‑CSF Biotinylated Antibody (Catalog # BAF214)
  • Standard: Recombinant Human G-CSF Protein (Catalog # 214-CS)
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 5 reviews rated 4.4 using MAB214 in the following applications:

Published Applications

Read 3 publications using MAB214 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: G-CSF

G-CSF is a pleiotropic cytokine best known for its specific effects on the proliferation, differentiation, and activation of hematopoietic cells of the neutrophilic granulocyte lineage. It is produced mainly by monocytes and macrophages upon activation by endotoxin, TNF-alpha and IFN-gamma. Other cell types including fibroblasts, endothelial cells, astrocytes and bone marrow stromal cells can also secrete G-CSF after LPS, IL-1, or TNF-alpha activation. In addition, various carcinoma cell lines and myeloblastic leukemia cells can express G-CSF constitutively.

In humans, two distinct cDNA clones for G-CSF, encoding 207 and 204 amino acid precursor proteins, have been isolated. Both proteins have a 30 amino acid signal peptide and have identical amino acid sequences except for a three amino acid insertion (deletion) at the 35th amino acid residue from the N-terminus of the mature protein. Human G-CSF is 73% identical at the amino acid level to murine G-CSF and the two proteins show species cross-reactivity.

In vitro, G-CSF stimulates growth, differentiation and functions of cells from the neutrophil lineage. It also has blast cell growth factor activity and can synergize with IL-3 to shorten the Go period of early hematopoietic progenitors. Consistent with its in vitro functions, G-CSF has been found to play important roles in defense against infection, in inflammation and repair, and in the maintenance of steady state hematopoiesis.

Long Name

Granulocyte Colony Stimulating Factor

Alternate Names

C17orf33, CSF3, CSF3OS, Filgrastim, GCSF, Lenograstim, Pluripoietin

Entrez Gene IDs

1440 (Human); 12985 (Mouse)

Gene Symbol

CSF3

UniProt

Additional G-CSF Products

Product Documents for Human G‑CSF Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human G‑CSF Antibody

For research use only

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Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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