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Recombinant Human G-CSF Protein Best Seller

Catalog # 214-CS | R&D Systems, Inc. a Bio-Techne Brand
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Carrier Free
214-CS-005/CF
214-CS-025/CF

Catalog #
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With Carrier
214-CS-005
214-CS-025

Key Product Details

Source

E. coli

Accession #

Conjugate

Unconjugated

Applications

Bioactivity

Product Specifications

Source

E. coli-derived human G-CSF protein
Thr31-Pro204, with an N-terminal Met

Purity

>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Met

Predicted Molecular Mass

18.8 kDa

SDS-PAGE

17-19 kDa, reducing conditions

Activity

Measured in a cell proliferation assay using NFS‑60 mouse myelogenous leukemia lymphoblast cells. Shirafuji, N.et al. (1989) Exp. Hematol. 17:116.
The ED50 for this effect is 10-60 pg/mL.

Scientific Data Images for Recombinant Human G-CSF Protein

Recombinant Human G-CSF Protein Bioactivity

Recombinant Human G-CSF Protein Bioactivity

Recombinant Human G-CSF (Catalog # 214-CS) stimulates cell proliferation of the NFS-60 mouse myelogenous leukemia lymphoblast cell line. The ED50 for this effect is 10-60 pg/mL.
Recombinant Human G-CSF Protein SDS-PAGE

Recombinant Human G-CSF Protein SDS-PAGE

1 μg/lane of Recombinant Human G-CSF was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing a single band at 18.5 kDa.

Formulation, Preparation and Storage

Carrier Free
What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

Carrier: 214-CS
Formulation Lyophilized from a 0.2 μm filtered solution in Acetic Acid with BSA as a carrier protein.
Reconstitution Reconstitute at 50 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Carrier Free: 214-CS/CF
Formulation Supplied as a 0.2 μm filtered solution in Acetic Acid.
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Do not freeze.
  • 6 months from date of receipt, 2 to 8 °C as supplied.

Background: G-CSF

G-CSF is a pleiotropic cytokine best known for its specific effects on the proliferation, differentiation, and activation of hematopoietic cells of the neutrophilic granulocyte lineage. It is produced mainly by monocytes and macrophages upon activation by endotoxin, TNF-alpha and IFN-gamma. Other cell types including fibroblasts, endothelial cells, astrocytes and bone marrow stromal cells can also secrete G-CSF after LPS, IL-1 or TNF-alpha activation. In addition, various carcinoma cell lines and myeloblastic leukemia cells can express G-CSF constitutively.

In humans, two distinct cDNA clones for G-CSF, encoding 207 and 204 amino acid precursor proteins, have been isolated. Both proteins have a 30 amino acid signal peptide and have identical amino acid sequences except for a three amino acid insertion (deletion) at the 35th amino acid residue from the N-terminus of the mature protein. Human G-CSF is 73% identical at the amino acid level to murine G-CSF and the two proteins show species cross-reactivity.

In vitro, G-CSF stimulates growth, differentiation and functions of cells from the neutrophil lineage. It also has blast cell growth factor activity and can synergize with IL-3 to shorten the Go period of early hematopoietic progenitors. Consistent with its in vitro functions, G-CSF has been found to play important roles in defense against infection, in inflammation and repair, and in the maintenance of steady state hematopoiesis.

Long Name

Granulocyte Colony Stimulating Factor

Alternate Names

C17orf33, CSF3, CSF3OS, Filgrastim, GCSF, Lenograstim, Pluripoietin

Entrez Gene IDs

1440 (Human); 12985 (Mouse)

Gene Symbol

CSF3

UniProt

Additional G-CSF Products

Product Documents for Recombinant Human G-CSF Protein

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Recombinant Human G-CSF Protein

For research use only

Citations for Recombinant Human G-CSF Protein (53)

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Customer Reviews for Recombinant Human G-CSF Protein (2)

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  • Recombinant Human G-CSF Protein
    Name: Meilyn Sylvestre
    Application: Stem/Immune cell maintenance or differentiation
    Verified Customer | Posted 11/20/2020
  • Recombinant Human G-CSF Protein
    Name: Anonymous
    Application: Immunoassay Standard
    Verified Customer | Posted 11/06/2018
    The recombinant G-CSF was used as a calibrator in an ELISA with sensitivity at 18pg/ml. The RnD Systems G-CSF antibody MAB214 was used as the capture in this assay along with BAF214 as the detection.
    Recombinant Human G-CSF Protein 214-CS

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