Human FCRL2/FcRH2, FCRL5/FcRH5 Antibody

Name: Human FCRL2/FcRH2, FCRL5/FcRH5 Antibody
Brand: R&D Systems
SKU: MAB2048

R&D Systems, Inc. a Bio-Techne Brand

Catalog # MAB2048

Citations (2)

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Clone 296902 was used by HLDA to establish CD designation

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Key Product Details

Species Reactivity:	Human
Immunogen:	Mouse myeloma cell line NS0-derived recombinant human FCRL2/FcRH2 Glu15-Asp395 Accession # Q96LA5
Applications:	CyTOF-ready, Flow Cytometry, Western Blot
Label:	Unconjugated
Source:	Monoclonal Mouse IgG₁ Clone # 296902
Specificity:	Detects human FCRL2/FcRH2 and FCRL5/FcRH5 in Western blots. In Western blots, approximately 25% cross-reactivity with recombinant human FCRL1, FCRL3, and FCRL4 is observed.

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Applications for Human FCRL2/FcRH2, FCRL5/FcRH5 Antibody

	Recommended Concentration	Sample
Western Blot	1 µg/mL	Recombinant Human FCRL2/FcRH2 (Catalog # 2048-FC)
Flow Cytometry	2.5 µg/10⁶ cells	See below
CyTOF-ready	Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Please Note: Optimal dilutions should be determined by each laboratory for each application.

Scientific Data Examples for Human FCRL2/FcRH2, FCRL5/FcRH5 Antibody

	Flow Cytometry Detection of FCRL2/FcRH2, FCRL5/FcRH5 in Human PBMCs by Flow Cytometry. Human peripheral blood monocytes (PBMC) were labeled with Mouse Anti-Human FCRL2/FcRH2, FCRL5/FcRH5 Monoclonal Antibody (Catalog # MAB2048) followed by Allophycocyanin-conjugated Anti-Mouse IgG F(ab')₂Secondary Antibody (F0101B) and Human CD19 PE-conjugated Monoclonal Antibody (FAB4867P). Quadrant markers were set based on control antibody staining (MAB002).

Formulation, Preparation and Storage

Purification	Protein A or G purified from hybridoma culture supernatant
Reconstitution	Reconstitute at 0.5 mg/mL in sterile PBS. Reconstitution Buffer Available: Size / Price Qty Loading... RB01:
Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.

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Background: FCRL2/FcRH2

Fc receptor-like 2 (FCRL2), also known as FcRH2 and IRTA4, belongs to the family of glycoprotein homologs of classical immunoglobulin (Ig) Fc receptors. In human, the type I transmembrane FCRL protein family contains from three to nine immunoglobulin-like domains (1, 2). Mature human FcRH2 consists of a 382 amino acid (aa) extracellular domain (ECD) with four Ig-like C2-set domains, a 21 aa transmembrane segment, and an 86 aa cytoplasmic domain with one ITAM-like, and two ITIM-like motifs (3‑5). Alternate splicing of human FCRL2 may generate isoforms with N-terminal, internal, or C-terminal deletions (4, 5). The gene for FcRH2 maps to the human Iq21-23 locus which is a hotspot for chromosomal translocation events associated with B cell malignancies (3, 6). Although there are several Fc receptor-like genes in the mouse, none of these is a clear ortholog to human FCRL2 (7). FCRL proteins are differentially expressed among B cells (2). FCRL2 is preferentially expressed on naïve and CD27⁺ memory B cells within the spleen, lymph nodes, tonsils, and peripheral blood (3, 4, 8, 9). It is also expressed on most B cells in B cell chronic lymphocytic leukemia (B-CLL) patients (10). FCRL2 upregulation is associated with mutation of the immunoglobulin heavy chain variable (IGHV) and less aggressive forms of B-CLL (9, 11).

Item	Value
References	Maltais, L.J. et al. (2006) Nat. Immunol. 7:431. Davis, R.S. et al. (2007) Annu. Rev. Immunol. 25:525. Miller, I. et al. (2002) Blood 99:2662. Davis, R.S. et al. (2001) Proc. Natl. Acad. Sci. 98:9772. Xu, M.J. et al. (2001) Biochem. Biophys. Res. Commun. 280:768. Hatzivassiliou, G. et al. (2001) Immunity 14:277. Davis, R.S. et al. (2004) Int. Immunol. 16:1343. Polson, A.G. et al. (2006) Int. Immunol. 18:1363. Huttmann, A. et al. (2006) Leukemia 20:1774. Kazemi, T. et al. (2008) Int. J. Cancer 123:2113. Li, F.J. et al. (2008) Blood 112:179.
Long Name	Fc Receptor-like 2
Entrez Gene IDs	79368 (Human)
Alternate Names	CD307b, CD307b antigen, FCRH2SPAP1B, FCRL2, Fc receptor homolog 2, Fc receptor-like 2, FcR-like protein 2, FcRH2, IFGP family protein 4, IFGP4, IRTA4, IRTA4SPAP1C, Immunoglobulin receptor translocation-associated protein 4, SH2 domain containing phosphatase anchor protein 1, SH2 domain-containing phosphatase anchor protein 1Fc receptor-like protein 2, SPAP1, SPAP1A, SPAP1immune receptor translocation-associated protein 4, immunoglobulin superfamily Fc receptor, gp42

Citations for Human FCRL2/FcRH2, FCRL5/FcRH5 Antibody (2)

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Citations are publications that use Bio-Techne products. Selected citations for Human FCRL2/FcRH2, FCRL5/FcRH5 Antibody include:

Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry

Cabezon R et al. (2011), Analysis of HLDA9 mAbs on plasmacytoid dendritic cells. Immunol. Lett., 134(2):167-73.
PMID: 20933012
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry

Matesanz-Isabel J et al. (2011), New B-cell CD molecules. Immunol. Lett., 134(2):104-12.
PMID: 20933010

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