This antibody binds to CHO cells transfected with human E-Selectin. It does not bind to COS cells transfected with human PECAM‑1, P‑Selectin, L‑Selectin, VCAM‑1, or ICAM‑1.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG₁

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Scientific Data Images for Human E-Selectin/CD62E Antibody

Detection of E-Selectin/CD62E in HUVECs by Flow Cytometry.

Human umbilical cord endothelial cells (HUVECs) were cultured for 6 hours in the presence of 25 ng/mL of rhTNF-alpha (210-TA, filled histogram) or rested (open histogram) and stained with Mouse anti-human E-selectin/CD62E (BBA26) followed by Goat anti-Mouse APC-conjugated secondary antibody (F0101B). Gates were set based on isotype control (MAB002, data not shown). Staining was performed using our Staining Membrane-associated Proteins protocol.

E‑Selectin/CD62E in HUVECs.

E-Selectin/CD62E was detected in immersion fixed HUVEC human umbilical vein endothelial cells stimulated with Recombinant Human TNF-alpha (Catalog # 210-TA) using Mouse Anti-Human E-Selectin/CD62E Monoclonal Antibody (Catalog # BBA26) at 5 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Applications for Human E-Selectin/CD62E Antibody

Application

Recommended Usage

Adhesion Blockade

The adhesion of U937 human histiocytic lymphoma cells (5 x 10⁴ cells/well) to immobilized Recombinant Human E‑Selectin (Catalog # ADP1, 2 µg/mL, 100 µL/well) was maximally inhibited (80-100%) by 25 µg/mL of the antibody.

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

0.25 µg/10⁶ cells
Sample: HUVEC human umbilical vein endothelial cells treated with Recombinant Human TNF‑ alpha (Catalog # 210-TA)

Immunocytochemistry

8-25 µg/mL
Sample: Immersion fixed HUVEC human umbilical vein endothelial cells stimulated with Recombinant Human TNF-alpha (Catalog # 210-TA)

Immunofluorescence

Pigott, R. et al. (1991) J. Immunol. 147:130.

Immunoprecipitation

Pigott, R. et al. (1991) J. Immunol. 147:130.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: E-Selectin/CD62E

E-Selectin, also known as ELAM-1, is a transmembrane glycoprotein that is transiently expressed on activated vascular endothelial cells. E-Selectin binds to sialyated, fucosylated molecules on neutrophils, monocytes, and a subset of memory T cells.

Alternate Names

CD62E, ELAM1, LECAM2, SELE

Entrez Gene IDs

6401 (Human); 20339 (Mouse); 25544 (Rat); 102143564 (Cynomolgus Monkey)

Gene Symbol

SELE

Additional E-Selectin/CD62E Products

Product Documents for Human E-Selectin/CD62E Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Human E-Selectin/CD62E Antibody

For research use only

Citations
Reviews

Calculators
Flow Cytometry

Human E-Selectin/CD62E Antibody

R&D Systems, part of Bio-Techne | Catalog # BBA26

Key Product Details

Species Reactivity

Validated:

Cited:

Applications