Human Dectin-1/CLEC7A APC-conjugated Antibody

Name: Human Dectin-1/CLEC7A APC-conjugated Antibody
Brand: R&D Systems
SKU: FAB1859A

Catalog # FAB1859A

Citations (5)

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Clone 259931 was used by HLDA to establish CD designation

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Species Reactivity:	Human
Immunogen:	Mouse myeloma cell line NS0-derived recombinant human Dectin‑1/CLEC7A Thr66-Met201 Accession # NP_072092
Applications:	Flow Cytometry
Label:	Allophycocyanin (Excitation=620-650 nm, Emission=660-670 nm)
Source:	Monoclonal Mouse IgG_2B Clone # 259931
Specificity:	Detects human Dectin‑1/CLEC7A in direct ELISAs. In directs ELISAs, less than 10% cross-reactivity with recombinant human (rh) DLEC and no cross-reactivity with recombinant mouse Dectin-1 or rhDectin-2 is observed.

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Applications

	Recommended Concentration	Sample
Flow Cytometry	10 µL/10⁶ cells	See below

Please Note: Optimal dilutions should be determined by each laboratory for each application.

Human Dectin-1/CLEC7A APC-conjugated Antibody Scientific Data Examples

	Flow Cytometry Detection of Dectin‑1/CLEC7A in Human Blood Monocytes by Flow Cytometry. Human peripheral blood monocytes were stained with Mouse Anti-Human Dectin-1/CLEC7A APC-conjugated Monoclonal Antibody (Catalog # FAB1859A, filled histogram) or isotype control antibody (Catalog # IC0041A, open histogram). View our protocol for Staining Membrane-associated Proteins.

Formulation, Preparation and Storage

Purification	Protein A or G purified from hybridoma culture supernatant
Formulation	Supplied in a saline solution containing BSA and Sodium Azide.
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied.

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Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

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Background: Dectin-1/CLEC7A

Dectin-1, also known as CLEC7A and the beta -glucan receptor, is a 33 kDa type II transmembrane C-type lectin that participates in the innate immune response to fungal pathogens. Although Dectin-1 structurally resembles other CLEC molecules, it binds its ligands in a calcium-independent manner (1, 2). Mature human Dectin-1 consists of a short N-terminal ITAM-containing cytoplasmic tail, a transmembrane segment, and a C-terminal stalk with a carbohydrate recognition domain (CRD) in the extracellular domain (3, 4). Alternate splicing generates one major splice form that lacks the stalk region (3‑5). This isoform is expressed on the surface of monocytes, macrophages, myeloid DC, neutrophils, eosinophils, B cells, and CD4⁺ T cells (6). The CRD selectively binds beta -glucan polymers, a major component of yeast and mycobacterial cell walls (5‑7). Yeast beta -glucan is accessible to Dectin‑1 only during the process of cell budding. Dectin-1 does not recognize the filamentous form of yeast (8). Dectin-1 mediates the phagocytosis of zymosan particles and intact yeast (8‑10). In the membrane, Dectin-1 colocalizes with TLR2 in the presence of zymosan, and the two receptors cooperate in ligand recognition and the propagation of proinflammatory signaling (9, 11‑13). Dectin-1 also interacts with tetraspanin CD37. This increases its stability on the cell membrane and inhibits ligand-induced signaling (14). Dectin-1 knockout mice show increased susceptibility to pathogenic infection (15‑16). The CRD of human Dectin-1 shares 77%, 60%, and 60% amino acid (aa) sequence identity with that of bovine, mouse and rat Dectin-1, respectively. It shares 29%‑39% aa sequence identity with the CRD of other subgroup members, including CLEC-1, CLEC-2, CLEC9A, CLEC12B, LOX-1, and MICL.

Item	Value
References	Kanazawa, N. (2007) J. Dermatol. Sci. 45:77. Brown, G.D. (2006) Nat. Rev. Immunol. 6:33. Hernanz-Falcon, P. et al. (2001) Immunogenetics 53:288. Yokota, K. et al. (2001) Gene 272:51. Willment, J.A. et al. (2001) J. Biol. Chem. 276:43818. Willment, J.A. et al. (2005) Eur. J. Immunol. 35:1539. Palma, A.S. et al. (2006) J. Biol. Chem. 281:5771. Gantner, B.N. et al. (2005) EMBO J. 24:1277. Gantner, B.N. et al. (2003) J. Exp. Med. 197:1107. Kennedy, A.D. et al. (2007) Eur. J. Immunol. 37:467. Brown, G.D. et al. (2003) J. Exp. Med. 197:1119. Yadav, M. and J.S. Schorey (2006) Blood 108:3168. Suram, S. et al. (2006) J. Biol. Chem. 281:5506. Meyer-Wentrup, F. et al. (2007) J. Immunol. 178:154. Saijo, S. et al. (2007) Nat. Immunol. 8:39. Taylor, P.R. et al. (2007) Nat. Immunol. 8:31.
Entrez Gene IDs	64581 (Human); 56644 (Mouse)
Alternate Names	BGR, Beta-glucan receptor, C-type (calcium dependent, carbohydrate-recognition domain) lectin, superfamilymember 12, C-type lectin domain family 7 member A, C-type lectin domain family 7, member A, C-type lectin superfamily member 12, CD369, CLEC7A, CLECSF12, CLECSF12DC-associated C-type lectin 1, DECTIN1CANDF4, Dectin-1, Dectin1, Dendritic cell-associated C-type lectin 1, dendritic cell-associated C-type lectin-1, hDectin-1, lectin-like receptor 1

Citations for Human Dectin-1/CLEC7A APC-conjugated Antibody (5)

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Citations are publications that use Bio-Techne products. Selected citations for Human Dectin-1/CLEC7A APC-conjugated Antibody include:

Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry

JM Tam et al. (2019), Tetraspanin CD82 Organizes Dectin-1 into Signaling Domains to Mediate Cellular Responses to Candida albicans J. Immunol., 0(0).
PMID: 31010852
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry

MS Gresnigt et al. (2018), Genetic deficiency of NOD2 confers resistance to invasive aspergillosis Nat Commun, 9(1):2636.
PMID: 29980664
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry

MS Gresnigt et al. (2017), The Absence of NOD1 Enhances Killing of Aspergillus fumigatus Through Modulation of Dectin-1 Expression Front Immunol, 8(0):1777.
PMID: 29326692
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry

Ofer Levy (2016), Age-Specific Adjuvant Synergy: Dual TLR7/8 and Mincle Activation of Human Newborn Dendritic Cells Enables Th1 Polarization J. Immunol., 0(0).
PMID: 27793997
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry

Kleinnijenhuis J et al. (0), Long-lasting effects of BCG vaccination on both heterologous Th1/Th17 responses and innate trained immunity. J Innate Immu6(2):152-8.
PMID: 24192057

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Showing 1 - 5 of 5 citations Showing All

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