Human Dectin-1/CLEC7A Antibody

Name: Human Dectin-1/CLEC7A Antibody
Brand: R&D Systems
SKU: MAB1859
Rating: 5 (2 reviews)

R&D Systems, Inc. a Bio-Techne Brand

Catalog # MAB1859

Citations (24)

(2)

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Clone 259931 was used by HLDA to establish CD designation

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Key Product Details

Species Reactivity:	Human
Immunogen:	Mouse myeloma cell line NS0-derived recombinant human Dectin‑1/CLEC7A Thr66-Met201 Accession # NP_072092
Applications:	Block/Neutralize, CyTOF-ready, Flow Cytometry, Immunocytochemistry/Immunofluorescence
Label:	Unconjugated
Source:	Monoclonal Mouse IgG_2B Clone # 259931
Specificity:	Detects human Dectin‑1/CLEC7A in direct ELISAs. In directs ELISAs, less than 10% cross-reactivity with recombinant human (rh) DLEC and no cross-reactivity with recombinant mouse Dectin-1 or rhDectin-2 is observed.

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Applications for Human Dectin-1/CLEC7A Antibody

	Recommended Concentration	Sample
Flow Cytometry	2.5 µg/10⁶ cells	Human whole blood monocytes
Blockade of Receptor-ligand Interaction	In a functional ELISA, 0.1-0.4 µg/mL of this antibody will block 50% of the binding of 75 ng/mL of biotinylated Laminarin to immobilized Recombinant Human Dectin-1/CLEC7A (Catalog # 1859-DC) coated at 250 ng/mL (100 µL/well). At 3 μg/mL, this antibody will block >90% of the binding.
CyTOF-ready	Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Immunocytochemistry	8-25 µg/mL	See below

Please Note: Optimal dilutions should be determined by each laboratory for each application.

Scientific Data Examples for Human Dectin-1/CLEC7A Antibody

	Immunocytochemistry Dectin‑1/CLEC7A in Human Dendritic Cells. Dectin-1/CLEC7A was detected in immersion fixed mature human dendritic cells using Mouse Anti-Human Dectin-1/CLEC7A Monoclonal Antibody (Catalog # MAB1859) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (yellow; Catalog # NL007) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Formulation, Preparation and Storage

Purification	Protein A or G purified from hybridoma culture supernatant
Endotoxin level	<0.10 EU per 1 μg of the antibody by the LAL method.
Reconstitution	Reconstitute at 0.5 mg/mL in sterile PBS. Reconstitution Buffer Available: Size / Price Qty Loading... RB01:
Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.

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Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

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Background: Dectin-1/CLEC7A

Dectin-1, also known as CLEC7A and the beta -glucan receptor, is a 33 kDa type II transmembrane C-type lectin that participates in the innate immune response to fungal pathogens. Although Dectin-1 structurally resembles other CLEC molecules, it binds its ligands in a calcium-independent manner (1, 2). Mature human Dectin-1 consists of a short N-terminal ITAM-containing cytoplasmic tail, a transmembrane segment, and a C-terminal stalk with a carbohydrate recognition domain (CRD) in the extracellular domain (3, 4). Alternate splicing generates one major splice form that lacks the stalk region (3‑5). This isoform is expressed on the surface of monocytes, macrophages, myeloid DC, neutrophils, eosinophils, B cells, and CD4⁺ T cells (6). The CRD selectively binds beta -glucan polymers, a major component of yeast and mycobacterial cell walls (5‑7). Yeast beta -glucan is accessible to Dectin‑1 only during the process of cell budding. Dectin-1 does not recognize the filamentous form of yeast (8). Dectin-1 mediates the phagocytosis of zymosan particles and intact yeast (8‑10). In the membrane, Dectin-1 colocalizes with TLR2 in the presence of zymosan, and the two receptors cooperate in ligand recognition and the propagation of proinflammatory signaling (9, 11‑13). Dectin-1 also interacts with tetraspanin CD37. This increases its stability on the cell membrane and inhibits ligand-induced signaling (14). Dectin-1 knockout mice show increased susceptibility to pathogenic infection (15‑16). The CRD of human Dectin-1 shares 77%, 60%, and 60% amino acid (aa) sequence identity with that of bovine, mouse and rat Dectin-1, respectively. It shares 29%‑39% aa sequence identity with the CRD of other subgroup members, including CLEC-1, CLEC-2, CLEC9A, CLEC12B, LOX-1, and MICL.

Item	Value
References	Kanazawa, N. (2007) J. Dermatol. Sci. 45:77. Brown, G.D. (2006) Nat. Rev. Immunol. 6:33. Hernanz-Falcon, P. et al. (2001) Immunogenetics 53:288. Yokota, K. et al. (2001) Gene 272:51. Willment, J.A. et al. (2001) J. Biol. Chem. 276:43818. Willment, J.A. et al. (2005) Eur. J. Immunol. 35:1539. Palma, A.S. et al. (2006) J. Biol. Chem. 281:5771. Gantner, B.N. et al. (2005) EMBO J. 24:1277. Gantner, B.N. et al. (2003) J. Exp. Med. 197:1107. Kennedy, A.D. et al. (2007) Eur. J. Immunol. 37:467. Brown, G.D. et al. (2003) J. Exp. Med. 197:1119. Yadav, M. and J.S. Schorey (2006) Blood 108:3168. Suram, S. et al. (2006) J. Biol. Chem. 281:5506. Meyer-Wentrup, F. et al. (2007) J. Immunol. 178:154. Saijo, S. et al. (2007) Nat. Immunol. 8:39. Taylor, P.R. et al. (2007) Nat. Immunol. 8:31.
Entrez Gene IDs	64581 (Human); 56644 (Mouse)
Alternate Names	BGR, Beta-glucan receptor, C-type (calcium dependent, carbohydrate-recognition domain) lectin, superfamilymember 12, C-type lectin domain family 7 member A, C-type lectin domain family 7, member A, C-type lectin superfamily member 12, CD369, CLEC7A, CLECSF12, CLECSF12DC-associated C-type lectin 1, DECTIN1CANDF4, Dectin-1, Dectin1, Dendritic cell-associated C-type lectin 1, dendritic cell-associated C-type lectin-1, hDectin-1, lectin-like receptor 1

Citations for Human Dectin-1/CLEC7A Antibody (24)

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Citations are publications that use Bio-Techne products. Selected citations for Human Dectin-1/CLEC7A Antibody include:

Species: Human
Sample Types: Whole Cells
Applications: Neutralization

N Rochereau et al. (2021), Essential role of TOSO/FAIM3 in intestinal IgM reverse transcytosis Cell Reports, 37(7):110006.
PMID: 34788614
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot

X He et al. (2021), LYSMD3: A mammalian pattern recognition receptor for chitin Cell Reports, 36(3):109392.
PMID: 34289364
Species: Human
Sample Types: Whole Cells
Applications: Neutralization

JC de Quaglia et al. (2019), Involvement of the Dectin-1 Receptor upon the Effector Mechanisms of Human Phagocytic Cells against Paracoccidioides brasiliensis J Immunol Res, 2019(0):1529189.
PMID: 30882002
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

JC Silva et al. (2019), Mac-1 triggers neutrophil DNA extracellular trap formation to Aspergillus fumigatus independently of PAD4 histone citrullination J. Leukoc. Biol., 0(0).
PMID: 31478251
Species: Human
Sample Types: Whole Cells
Applications: Neutralization

MMM Kaisar et al. (2018), Dectin-1/2-induced autocrine PGE2 signaling licenses dendritic cells to prime Th2 responses PLoS Biol., 16(4):e2005504.
PMID: 29668708
Species: Human
Sample Types: Whole Cells
Applications: Neutralization

B Kan et al. (2018), Cellular metabolism constrains innate immune responses in early human ontogeny Nat Commun, 9(1):4822.
PMID: 30446641
Species: Human
Sample Types: Whole Cells
Applications: Neutralization

CC Yeh et al. (2017), Dectin-1-Mediated Pathway Contributes to Fusarium proliferatum-Induced CXCL-8 Release from Human Respiratory Epithelial Cells Int J Mol Sci, 18(3).
PMID: 28335387
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot

M Swidergall et al. (2017), EphA2 is an epithelial cell pattern recognition receptor for fungal ?-glucans Nat Microbiol, 0(0).
PMID: 29133884
Species: Human
Sample Types: Whole Cells
Applications: Neutralization

Gazendam R et al. (2016), Human Neutrophils Use Different Mechanisms To Kill Aspergillus fumigatus Conidia and Hyphae: Evidence from Phagocyte Defects. J Immunol, 196(3):1272-83.
PMID: 26718340
Species: Human
Sample Types: Whole Cells
Applications: Neutralization

(2016), Kluyveromyces marxianus and Saccharomyces boulardii Induce Distinct Levels of Dendritic Cell Cytokine Secretion and Significantly Different T Cell Responses In Vitro PLoS ONE, 11(11):e0167410.
PMID: 27898740
Species: Human
Sample Types: Whole Cells
Applications: Neutralization

Elder M et al. (2015), TSLP production by dendritic cells is modulated by IL-1beta and components of the endoplasmic reticulum stress response. Eur J Immunol, 46(2):455-63.
PMID: 26573878
Species: Human
Sample Types: Whole Cells
Applications: Neutralization

Joo H et al. (2015), Opposing Roles of Dectin-1 Expressed on Human Plasmacytoid Dendritic Cells and Myeloid Dendritic Cells in Th2 Polarization. J Immunol, 195(4):1723-31.
PMID: 26123355
Species: Human
Sample Types: Whole Cells
Applications: Blocking

Fernandes R et al. (2015), Paracoccidioides brasiliensis interferes on dendritic cells maturation by inhibiting PGE2 production. PLoS ONE, 10(3):e0120948.
PMID: 25793979
Species: Human
Sample Types: Whole Cells
Applications: Neutralization

Rodrigues D et al. (2014), Interferon-gamma production by human neutrophils upon stimulation by IL-12, IL-15 and IL-18 and challenge with Paracoccidioides brasiliensis. Cytokine, 69(1):102-9.
PMID: 25022968
Species: Human
Sample Types: Whole Cells
Applications: ICC

Baranov et al. (2014), Podosomes of dendritic cells facilitate antigen sampling. J Cell Sci, 127(0):1052-64.
PMID: 24424029
Species: Human
Sample Types: Whole Cells
Applications: ICC

Cohen-Kedar S et al. (2014), Human intestinal epithelial cells respond to beta-glucans via Dectin-1 and Syk. Eur J Immunol, 44(12):3729-40.
PMID: 25251945
Species: Human
Sample Types: Whole Cells
Applications: Neutralization

Ifrim D et al. (2013), Candida albicans primes TLR cytokine responses through a Dectin-1/Raf-1-mediated pathway. J Immunol, 190(8):4129-35.
PMID: 23475217
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry

Lefevre L et al. (2013), The C-type lectin receptors dectin-1, MR, and SIGNR3 contribute both positively and negatively to the macrophage response to Leishmania infantum. Immunity, 38(5):1038-49.
PMID: 23684988
Species: Human
Sample Types: Whole Cells
Applications: Neutralization

Gringhuis SI et al. (2012), Dectin-1 is an extracellular pathogen sensor for the induction and processing of IL-1beta via a noncanonical caspase-8 inflammasome. Nat. Immunol., 13(3):246-54.
PMID: 22267217
Species: Human
Sample Types: Whole Cells
Applications: Neutralization

Li D et al. (2012), MBL-mediated opsonophagocytosis of Candida albicans by human neutrophils is coupled with intracellular Dectin-1-triggered ROS production. PLoS ONE, 7(12):e50589.
PMID: 23239982
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry

Kassianos AJ et al. (2012), Human CD1c (BDCA-1)+ myeloid dendritic cells secrete IL-10 and display an immuno-regulatory phenotype and function in response to Escherichia coli. Eur. J. Immunol., 42(6):1512-22.
PMID: 22678905
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry

Cabezon R et al. (2011), Analysis of HLDA9 mAbs on plasmacytoid dendritic cells. Immunol. Lett., 134(2):167-73.
PMID: 20933012
Species: Human
Sample Types: Whole Cells
Applications: Neutralization

Gringhuis SI et al. (2011), Selective C-Rel activation via Malt1 controls anti-fungal T(H)-17 immunity by dectin-1 and dectin-2. PLoS Pathog., 7(1):e1001259.
PMID: 21283787
Species: Human
Sample Types: Cell Lysates
Applications: Immunoprecipitation

Elsori DH et al. (2011), Protein kinase Cdelta is a critical component of Dectin-1 signaling in primary human monocytes. J. Leukoc. Biol., 90(3):599-611.
PMID: 21653233

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Customer Reviews for Human Dectin-1/CLEC7A Antibody (2)

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Human Dectin-1/CLEC7A Antibody

Name: Anonymous

Application: Flow

Sample Tested: THP-1 human acute monocytic leukemia cell line

Species: Human

Verified Customer | Posted 11/28/2020

Dectin1 is detected in THP1 monocytes using 100 nM of the Human Dectin-1/CLEC7A Antibody clone 259931. The 259931 antibody was detected with a secondary goat anti-mouse IgG Fc Alexa Fluor 647 antibody.
Human Dectin-1/CLEC7A Antibody

Name: Anonymous

Application: Flow

Sample Tested: macrophages

Species: Human

Verified Customer | Posted 10/13/2020

Detection of human Dectin1 in monocyte-derived cultured macrophages by Flow Cytometry. Human monocytes were cultured in MCSF for 6 days to differentiate into macrophages. After differentiation, the macrophages were stained with the mouse anti-human Dectin1 antibody clone 259931 at 100 nM or the mouse IgG2b isotype control. Binding was detected with a secondary goat a-mouse IgG Fc gamma fragment APC antibody.

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Product Documents for Human Dectin-1/CLEC7A Antibody

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Human Dectin-1/CLEC7A Antibody

R&D Systems, Inc. a Bio-Techne Brand

Key Product Details

Complete Your Research

Complete Your Experiment

Applications for Human Dectin-1/CLEC7A Antibody

Scientific Data Examples for Human Dectin-1/CLEC7A Antibody

Immunocytochemistry

Formulation, Preparation and Storage

Plan Your Experiments

Reconstitution Calculator

Background: Dectin-1/CLEC7A

Citations for Human Dectin-1/CLEC7A Antibody (24)

Customer Reviews for Human Dectin-1/CLEC7A Antibody (2)

Customer Images

Human Dectin-1/CLEC7A Antibody

Human Dectin-1/CLEC7A Antibody

Product Documents for Human Dectin-1/CLEC7A Antibody

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