Key Product Details
Product Summary for Human DC‑SIGN/CD209 Antibody
Scientific Data Images for Human DC‑SIGN/CD209 Antibody
Detection of DC‑SIGN in Human DC‑SIGN Transfected 3T3 Mouse Cell Line by Flow Cytometry.Human DC-SIGN and DC-SIGN2 transfected 3T3 mouse embryonic fibroblast cell line were stained with Mouse Anti-Human DC-SIGN Monoclonal Antibody (Catalog # MAB161, filled histograms) or isotype control antibody (Catalog # MAB0041, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG F(ab')2Secondary Antibody (Catalog # F0102B).
Detection of DC‑SIGN in Human Monocyte Derived Dendritic Cells by Flow Cytometry.Human monocyte derived dendritic cells were stained with Mouse Anti-Human DC-SIGN Monoclonal Antibody (Catalog # MAB161) followed by PE-conjugated anti-mouse IgG (Catalog # F0102B) and Anti-Human B7-2/CD86 Fluorescein-conjugated Monoclonal Antibody (Catalog # FAB141F). Quadrant markers were set based on control antibody staining (Catalog # MAB0041).
Applications for Human DC‑SIGN/CD209 Antibody
Sample: Human DC‑SIGN transfected 3T3 mouse embryonic fibroblast cell line and human monocyte derived dendritic cells
Sample: Recombinant Human DC-SIGN Fc Chimera (Catalog # 161-DC)
Read 4 reviews rated 4.3 using MAB161 in the following applications:
Formulation, Preparation and Storage
Stability & Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Human DC-Sign (dendritic cell-specific ICAM-3 grabbing nonintegrin; also CD209) is a member of the chromosome 19 C-type lectin family that includes DC-SIGN, DC-SIGN-related protein, CD23 and LSECtin (1). DC-SIGN was initially reported to be a 46 kDa, 404 amino acid (aa) type II transmembrane protein that contained a 40 aa cytoplasmic N-terminus, a 21 aa transmembrane segment, and a 343 aa extracellular C-terminus (2). The extracellular region contains a distal, 115 aa Ca++-dependent carbohydrate-binding lectin domain and a membrane-proximal linker segment that is composed of seven 23 aa repeats (2, 3). The lectin domain is believed to preferably bind mannose, either within the context of ICAM-3 (on T cells) or ICAM-2 (on endothelial cells) (2, 4, 5). DC-SIGN expression appears to be limited to dendritic cells (DC) and macrophages (6), and DC interaction with the ICAMs both aids DC cell trafficking and immunological synapse formation (7). Since the original report on DC-SIGN, multiple splice forms have been discovered, generating both membrane-bound and soluble forms (3). There are eight type A isoforms, all of which begin with the same 15 aa of exon 1a. Four contain the transmembrane region of exon II, and four do not (i.e., are soluble). Among these eight type A isoforms, only three retain the entire 343 aa found in the full length form described in reference #2 (the full length form is referred to as type I mDC-SIGN1A) (3). Five additional isoforms utilize an alternate start site, and these are referred to as type B isoforms. These all show a 35 aa cytoplasmic domain. One also has a transmembrane segment; four do not. Two of the five contain full, unspliced extracellular regions (3). All of this suggests enormous complexity in DC-SIGN biology. DC-SIGN is not well conserved across species. Human and mouse show little overall aa identity. In the lectin domain, however, human DC-SIGN shares 68% aa identity with mouse DC-SIGN (8). Human and rhesus monkey DC-SIGN share 91% aa identity over the entire extracellular region (8). A detailed description of the additional properties of this monoclonal antibody (MAB161) have been published (9, 10).
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- Mummidi, S. et al. (2001) J. Biol. Chem. 276:33196.
- Su, S.V. et al. (2004) J. Biol. Chem. 279:19122.
- Cambi, A. et al. (2005) Cell. Microbiol. 7:481.
- Serrano-Gomez, D. et al. (2004) J. Immunol. 173:5635.
- Geijtenbeek, T.B.H. and Y. van Kooyk (2003) Curr. Top. Microbiol. Immunol. 276:32.
- Baribaud, F. et al. (2001) J. Virol. 75:10281.
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- Baribaud, F. et al. (2002) J. Virol.76:9135.
Product Specific Notices for Human DC‑SIGN/CD209 Antibody
For research use only