Human CXCR4 Antibody

Name: Human CXCR4 Antibody
Brand: R&D Systems
SKU: MAB170

R&D Systems, Inc. a Bio-Techne Brand

Catalog # MAB170

Citations (33)

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Key Product Details

Species Reactivity:	Human
Immunogen:	CP-MAC-infected SUP-T1 human T cell lymphoblastic lymphoma line Accession # P61073
Applications:	Block/Neutralize, CyTOF-reported, Flow Cytometry
Label:	Unconjugated
Source:	Monoclonal Mouse IgG_2A Clone # 12G5
Specificity:	Detects human CXCR4.

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Applications for Human CXCR4 Antibody

	Recommended Concentration	Sample
Flow Cytometry	0.25 µg/10⁶ cells	See below
CyTOF-reported	Nair, N. et al. (2016) Mucosal Immunol. 9: 68. Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Item	Value
Neutralization	Measured by its ability to neutralize CXCL12/SDF‑1 alpha -induced chemotaxis in the BaF3 mouse pro‑B cell line transfected with human CXCR4. The Neutralization Dose (ND₅₀) is typically 0.3-1.2 µg/mL in the presence of 1 ng/mL Recombinant Human/Feline/Rhesus Macaque CXCL12/SDF‑1 alpha.

Please Note: Optimal dilutions should be determined by each laboratory for each application.

Scientific Data Examples for Human CXCR4 Antibody

	Flow Cytometry Detection of CXCR4 in Human Blood Lymphocytes by Flow Cytometry. Human peripheral blood lymphocytes were stained with Mouse Anti-Human CD19 APC-conjugated Monoclonal Antibody (Catalog # FAB4867A) and either (A) Mouse Anti-Human CXCR4 Monoclonal Antibody (Catalog # MAB170) or (B) Mouse IgG_2AIsotype Control (Catalog # MAB003) followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B). View our protocol for Staining Membrane-associated Proteins.
	Neutralization Chemotaxis Induced by CXCL12/SDF-1 alpha and Neutrali-zation by Human CXCR4 Antibody. Recombinant Human/ Feline/Rhesus Macaque CXCL12/SDF-1a (Catalog # 350-NS) chemoattracts the BaF3 mouse pro-B cell line transfected with human CXCR4 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Human/Feline/ Rhesus Macaque CXCL12/ SDF-1a (1 ng/mL) is neutralized (green line) by increasing concentrations of Human CXCR4 Monoclonal Antibody (Catalog # MAB170). The ND₅₀ is typically 0.3-1.2 µg/mL.

Formulation, Preparation and Storage

Purification	Protein A or G purified from ascites
Endotoxin level	<0.10 EU per 1 μg of the antibody by the LAL method.
Reconstitution	Reconstitute at 0.5 mg/mL in sterile PBS. Reconstitution Buffer Available: Size / Price Qty Loading... RB01:
Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.

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Background: CXCR4

CXCR4, also known as CD184, is a G-protein-linked seven transmembrane spanning receptor that binds stromal cell-derived factor-1 (SDF-1). CXCR4 acts as a co-factor for T-cell tropic HIV-1 and -2 viral entry into cells. While primarily a membrane protein, CXCR4 undergoes trafficking and internalization in response to stimulation with phorbol esters and ligand (1). Cytoplasmic and nuclear localization of CXCR4 has been observed in colorectal and renal carcinomas (2,3) and it has been used as the basis of prognosis and metastatic state (3,4,5).

Item	Value
References	Orsini, M.J. et al. (1999) J. Biol. Chem. 274:31076. Zagzag, D. et al. (2005) Cancer Res. 65:6178. Speetjens, F.M. et al. (2009) Cancer Microenvironment 2:1. Wang, L. et al. (2009) Oncology Reports 22:1333. Amara, S. et al. (2015) Cancer Biomark. 15:869.
Long Name	C-X-C Motif Chemokine Receptor 4
Entrez Gene IDs	7852 (Human); 12767 (Mouse); 60628 (Rat); 483900 (Canine); 493676 (Feline)
Alternate Names	C-X-C chemokine receptor type 4, CD184, CXC-R4, CXCR-4, CXCR4, D2S201E, D2S201ESDF-1 receptor, FB22, Fusin, HM89, HM89NPYRL, HSY3RR, LAP-3, LAP3, LCR1, LESTR, LESTRCD184 antigen, Leukocyte-derived seven transmembrane domain receptor, NPY3R, NPY3RWHIM, NPYR, NPYRL, NPYY3R, Stromal cell-derived factor 1 receptor, WHIMS, chemokine (C-X-C motif) receptor 4, chemokine (C-X-C motif), receptor 4 (fusin), leukocyte-derived seven-transmembrane-domain receptor, lipopolysaccharide-associated protein 3, neuropeptide Y receptor Y3, pCXCR4, seven transmembrane helix receptor, seven-transmembrane-segment receptor, spleen

Citations for Human CXCR4 Antibody (33)

Showing 1 - 10 of 33 citations Showing All

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Citations are publications that use Bio-Techne products. Selected citations for Human CXCR4 Antibody include:

Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry

WG Lesniak et al. (2022), An Evaluation of CXCR4 Targeting with PAMAM Dendrimer Conjugates for Oncologic Applications Pharmaceutics, 14(3).
PMID: 35336029
Species: Human
Sample Types: Whole Cells
Applications: Binding Assay

M Armani-Tou et al. (2021), Mechanisms of HIV-1 evasion to the antiviral activity of chemokine CXCL12 indicate potential links with pathogenesis PloS Pathogens, 17(4):e1009526.
PMID: 33872329
Species: Human
Sample Types: Whole Tissue
Applications: IHC

G Cantini et al. (2021), Stimulated Expression of CXCL12 in Adrenocortical Carcinoma by the PPARgamma Ligand Rosiglitazone Impairs Cancer Progression Journal of personalized medicine, 11(11).
PMID: 34834449
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot

S Li et al. (2019), Activation of MAPK signaling by CXCR7 leads to enzalutamide resistance in prostate cancer Cancer Res., 0(0).
PMID: 30952632
Species: Human
Sample Types: Whole Cells
Applications: ICC

C Ieranò et al. (2019), CXCL12 loaded-dermal filler captures CXCR4 expressing melanoma circulating tumor cells Cell Death Dis, 10(8):562.
PMID: 31332163
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry

BA Dinkel et al. (2018), GRK2 mediates TCR-induced transactivation of CXCR4 and TCR-CXCR4 complex formation which drives PI3K?/PREX1 signaling and T cell cytokine secretion J. Biol. Chem., 0(0).
PMID: 30018141
Species: Human
Sample Types: Whole Cells
Applications: Neutralization

Y Han et al. (2017), CXCR7 maintains osteosarcoma invasion after CXCR4 suppression in bone marrow microenvironment Tumour Biol., 39(5):1010428317701.
PMID: 28468584
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry

WL Wu et al. (2017), ?20 IFITM2 differentially restricts X4 and R5 HIV-1 Proc. Natl. Acad. Sci. U.S.A., 114(27):7112-7117.
PMID: 28630320
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry

SC Mills et al. (2017), Rac1 plays a role in CXCL12 but not CCL3-induced chemotaxis and Rac1 GEF inhibitor NSC23766 has off target effects on CXCR4 Cell. Signal., 42(0):88-96.
PMID: 29050986
Species: Human
Sample Types: Whole Cells
Applications: Neutralization Control

(2016), HEREGULIN/ErbB3 SIGNALING ENHANCES CXCR4-DRIVEN Rac1 ACTIVATION AND BREAST CANCER CELL MOTILITY VIA HIF-1? Mol Cell Biol, 0(0).
PMID: 27185877
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

K Griffiths et al. (2016), I-bodies: human single domain antibodies that antagonize chemokine receptor CXCR4 J Biol Chem, 0(0).
PMID: 27036939
Species: Human
Sample Types: Whole Cells
Applications: IHC-Fr

(2016), Signal transmission through the CXC chemokine receptor 4 (CXCR4) transmembrane helices Proc Natl Acad Sci USA, 0(0).
PMID: 27543332
Species: Human
Sample Types: Whole Cells
Applications: Neutralization

Shankar V et al. (2015), Mesenchymal stromal cell secretome up-regulates 47 kDa CXCR4 expression, and induce invasiveness in neuroblastoma cell lines. PLoS ONE, 10(3):e0120069.
PMID: 25774696
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry

Patrussi L et al. (2015), Enhanced Chemokine Receptor Recycling and Impaired S1P1 Expression Promote Leukemic Cell Infiltration of Lymph Nodes in Chronic Lymphocytic Leukemia. Cancer Res, 75(19):4153-63.
PMID: 26282174
Species: Human
Sample Types: Whole Cells
Applications: Neutralization

Zsiros E et al. (2015), The Ovarian Cancer Chemokine Landscape Is Conducive to Homing of Vaccine-Primed and CD3/CD28-Costimulated T Cells Prepared for Adoptive Therapy. Clin Cancer Res, 21(12):2840-50.
PMID: 25712684
Species: Human
Sample Types: Whole Cells
Applications: Neutralization

Park B et al. (2013), An isoform of C/EBPbeta, LIP, regulates expression of the chemokine receptor CXCR4 and modulates breast cancer cell migration. J Biol Chem, 288(40):28656-67.
PMID: 23966000
Species: Human
Sample Types: Whole Cells
Applications: Neutralization

Yasumoto K et al. (2011), The EGFR ligands amphiregulin and heparin-binding egf-like growth factor promote peritoneal carcinomatosis in CXCR4-expressing gastric cancer. Clin. Cancer Res., 17(11):3619-30.
PMID: 21482691
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry

Hartl D et al. (2008), Infiltrated neutrophils acquire novel chemokine receptor expression and chemokine responsiveness in chronic inflammatory lung diseases. J. Immunol., 181(11):8053-67.
PMID: 19017998
Species: Human
Sample Types: Whole Cells
Applications: Neutralization

Kobune M et al. (2008), Interaction with human stromal cells enhances CXCR4 expression and engraftment of cord blood Lin(-)CD34(-) cells. Exp. Hematol., 36(9):1121-31.
PMID: 18562079
Species: Human
Sample Types: Whole Cells
Applications: Neutralization

Ao M et al. (2007), Cross-talk between paracrine-acting cytokine and chemokine pathways promotes malignancy in benign human prostatic epithelium. Cancer Res., 67(9):4244-53.
PMID: 17483336
Species: Mouse
Sample Types: In Vivo
Applications: Neutralization

Kaminski A et al. (2007), Endothelial NOS is required for SDF-1alpha/CXCR4-mediated peripheral endothelial adhesion of c-kit+ bone marrow stem cells. Lab. Invest., 88(1):58-69.
PMID: 18040270
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry

McLean AB et al. (2007), Activin a efficiently specifies definitive endoderm from human embryonic stem cells only when phosphatidylinositol 3-kinase signaling is suppressed. Stem Cells, 25(1):29-38.
PMID: 17204604
Species: Human
Sample Types: Whole Cells
Applications: Neutralization

Deiva K et al. (2006), CCR5-, DC-SIGN-dependent endocytosis and delayed reverse transcription after human immunodeficiency virus type 1 infection in human astrocytes. AIDS Res. Hum. Retroviruses, 22(11):1152-61.
PMID: 17147503
Species: Human
Sample Types: Whole Cells
Applications: ICC

Lisignoli G et al. (2006), Hyaluronan-based polymer scaffold modulates the expression of inflammatory and degradative factors in mesenchymal stem cells: Involvement of Cd44 and Cd54. J. Cell. Physiol., 207(2):364-73.
PMID: 16331675
Species: Human
Sample Types: Whole Tissue
Applications: IHC

Narducci MG et al. (2005), Skin homing of Sezary cells involves SDF-1-CXCR4 signaling and down-regulation of CD26/dipeptidylpeptidase IV. Blood, 107(3):1108-15.
PMID: 16204308
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry

Zhang Y et al. (2004), Intracellular localization and constitutive endocytosis of CXCR4 in human CD34+ hematopoietic progenitor cells. Stem Cells, 22(6):1015-29.
PMID: 15536192
Species: Human
Sample Types: Whole Cells
Applications: ICC

Tavor S et al. (2004), CXCR4 regulates migration and development of human acute myelogenous leukemia stem cells in transplanted NOD/SCID mice. Cancer Res., 64(8):2817-24.
PMID: 15087398
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry

Lawrence DM et al. (2004), Human immunodeficiency virus type 1 infection of human brain-derived progenitor cells. J. Virol., 78(14):7319-28.
PMID: 15220405
Species: Human
Sample Types: Whole Cells
Applications: ICC

Schneider P et al. (2002), Is high expression of the chemokine receptor CXCR-4 of predictive value for early relapse in childhood acute lymphoblastic leukaemia? Br. J. Haematol., 119(2):579-80.
PMID: 12406108
Species: Human
Sample Types: Whole Cells
Applications: ICC

Bug G et al. (2002), Rho family small GTPases control migration of hematopoietic progenitor cells into multicellular spheroids of bone marrow stroma cells. J. Leukoc. Biol., 72(4):837-45.
PMID: 12377954
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry

Honczarenko M et al. (2002), CCR5-binding chemokines modulate CXCL12 (SDF-1)-induced responses of progenitor B cells in human bone marrow through heterologous desensitization of the CXCR4 chemokine receptor. Blood, 100(7):2321-9.
PMID: 12239139
Species: Human
Sample Types: Whole Tissue
Applications: IHC-Fr

Huber TB et al. (2002), Expression of functional CCR and CXCR chemokine receptors in podocytes. J. Immunol., 168(12):6244-52.
PMID: 12055238
Species: Human
Sample Types: Whole Cells
Applications: Neutralization

Lin Y et al. (0), Mesenchymal Stem Cells Ameliorate Atherosclerotic Lesions via Restoring Endothelial Function. Stem Cells Transl Me4(1):44-55.
PMID: 25504897

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