Key Product Details
Product Summary for Human CXCL13/BLC/BCA‑1 Antibody
Accession # Q53X90
Scientific Data Images for Human CXCL13/BLC/BCA‑1 Antibody
Chemotaxis Induced by CXCL13/BLC/BCA‑1 and Neutralization by Human CXCL13/BLC/BCA‑1 Antibody.Recombinant Human CXCL13/BLC/BCA-1 (Catalog # 801-CX) chemoattracts the BaF3 mouse pro-B cell line transfected with human CXCR5 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin. Chemotaxis elicited by Recombinant Human CXCL13/BLC/BCA-1 (50 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human CXCL13/BLC/BCA-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF801). The ND50 is typically 1-4 µg/mL.
CXCL13/BLC/BCA‑1 in Human Lymphoma.CXCL13/BLC/BCA-1 was detected in immersion fixed paraffin-embedded sections of human lymphoma using Goat Anti-Human CXCL13/BLC/BCA-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF801) at 10 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Applications for Human CXCL13/BLC/BCA‑1 Antibody
Sample: Immersion fixed paraffin-embedded sections of human tonsil and human lymphoma
Sample: Recombinant Human CXCL13/BLC/BCA‑1 (Catalog # 801-CX)
Read 7 reviews rated 4.6 using AF801 in the following applications:
- Flow Cytometry (1 Review)
- Immunocytochemistry/Immunofluorescence (1 Review)
- Immunofluorescence - paraffin (1 Review)
- Immunohistochemistry (3 Reviews)
- Immunohistochemistry-Paraffin (1 Review)
Read 20 publications using AF801 in the following applications:
Formulation, Preparation and Storage
Stability & Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Product Specific Notices for Human CXCL13/BLC/BCA‑1 Antibody
For research use only