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Human CXCL13/BLC/BCA‑1 Antibody

Catalog # AF801 | R&D Systems, Inc. a Bio-Techne Brand
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AF801
AF801-SP

Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Neutralization, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Product Summary for Human CXCL13/BLC/BCA‑1 Antibody

Immunogen

E. coli-derived recombinant human CXCL13/BCA-1
Val23-Arg94
Accession # Q53X90

Specificity

Detects CXCL13/BLC/BCA‑1 in direct ELISAs and Western blots. 

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human CXCL13/BLC/BCA‑1 Antibody

Chemotaxis Induced by CXCL13/BLC/BCA‑1 and Neutralization by Human CXCL13/BLC/BCA‑1 Antibody.

Chemotaxis Induced by CXCL13/BLC/BCA‑1 and Neutralization by Human CXCL13/BLC/BCA‑1 Antibody.

Recombinant Human CXCL13/BLC/BCA-1 (Catalog # 801-CX) chemoattracts the BaF3 mouse pro-B cell line transfected with human CXCR5 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin. Chemotaxis elicited by Recombinant Human CXCL13/BLC/BCA-1 (50 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human CXCL13/BLC/BCA-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF801). The ND50 is typically 1-4 µg/mL.
CXCL13/BLC/BCA-1 antibody in Human Lymphoma by Immunohistochemistry (IHC-P).

CXCL13/BLC/BCA‑1 in Human Lymphoma.

CXCL13/BLC/BCA-1 was detected in immersion fixed paraffin-embedded sections of human lymphoma using Goat Anti-Human CXCL13/BLC/BCA-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF801) at 10 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Applications for Human CXCL13/BLC/BCA‑1 Antibody

Application
Recommended Usage

Immunohistochemistry

5-15 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human tonsil and human lymphoma

Western Blot

0.1 µg/mL
Sample: Recombinant Human CXCL13/BLC/BCA‑1 (Catalog # 801-CX)

Neutralization

Measured by its ability to neutralize CXCL13/BLC/BCA‑1-induced chemotaxis in the BaF3 mouse pro‑B cell line transfected with human CXCR5. The Neutralization Dose (ND50) is typically 1-4 µg/mL in the presence of 50 ng/mL Recombinant Human CXCL13/BLC/BCA‑1.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 7 reviews rated 4.6 using AF801 in the following applications:

Published Applications

Read 20 publications using AF801 in the following applications:

Formulation, Preparation and Storage

Purification

Antigen Affinity-purified

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CXCL13/BLC/BCA-1

CXCL13, also known as B-lymphocyte chemoattractant (BLC), is a CXC chemokine that is constitutively expressed in secondary lymphoid organs. BCA-1 cDNA encodes a protein of 109 amino acid residues with a leader sequence of 22 residues. Mature human BCA-1 shares 64% amino acid sequence similarity with the mouse protein and 23‑34% amino acid sequence identity with other known CXC chemokines. Recombinant or chemically synthesized BCA-1 is a potent chemoattractant for B lymphocytes but not T lymphocytes, monocytes or neutrophils. BLR1, a G protein-coupled receptor originally isolated from Burkitt’s lymphoma cells, has now been shown to be the specific receptor for BCA1. Among cells of the hematopoietic lineages, the expression of BLR1, now designated CXCR5, is restricted to B lymphocytes and a subpopulation of T helper memory cells. Mice lacking BLR1 have been shown to lack inguinal lymph nodes. These mice were also found to have impaired development of Peyer’s patches and defective formation of primary follicles and germinal centers in the spleen as a result of the inability of B lymphocytes to migrate into B cell areas.

References

  1. Gunn, M.D. et al. (1998) Nature, 391:799.
  2. Legler, D.F. et al. (1998) J. Exp. Med. 187:655.
  3. Forster, R. et al. (1996) Cell 87:1037.

Alternate Names

ANGIE2, BCA-1, BCA1, BLC, BLR1L, SCYB13

Entrez Gene IDs

10563 (Human); 55985 (Mouse)

Gene Symbol

CXCL13

UniProt

Product Documents for Human CXCL13/BLC/BCA‑1 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human CXCL13/BLC/BCA‑1 Antibody

For research use only

Citations for Human CXCL13/BLC/BCA‑1 Antibody (20)

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Customer Reviews for Human CXCL13/BLC/BCA‑1 Antibody (7)

4.6 out of 5
7 customer ratings
5 stars
57%
4 stars
43%
3 stars
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  • Human CXCL13/BLC/BCA-1 Antibody
    Name: Yannick Alexandre
    Application: Flow Cytometry
    Sample Tested: Splenocytes
    Species: Human
    Verified Customer | Posted 07/06/2022
    CXCL13 and negative control.
    Human CXCL13/BLC/BCA‑1 Antibody AF801
  • Human CXCL13/BLC/BCA-1 Antibody
    Name: Yannick Alexandre
    Application: Immunocytochemistry/Immunofluorescence
    Sample Tested: Spleen tissue
    Species: Human
    Verified Customer | Posted 07/06/2022
    Human spleens fixed in 4% PFA
    Human CXCL13/BLC/BCA‑1 Antibody AF801
  • Human CXCL13/BLC/BCA-1 Antibody
    Name: Anonymous
    Application: Immunohistochemistry
    Sample Tested: Tonsil tissue
    Species: Human
    Verified Customer | Posted 12/10/2021
    Tonsil FFPE section. Primary Ab: 2ug/mL Detected using BOND Refine kit Rabbit anti-goat was used as Post Primary ER2 retrieval
    Human CXCL13/BLC/BCA‑1 Antibody AF801
  • Human CXCL13/BLC/BCA-1 Antibody
    Name: Anonymous
    Application: Immunofluorescence - paraffin
    Sample Tested: Diffuse large B cell lymphoma
    Species: Human
    Verified Customer | Posted 01/16/2020
    pH 9 antigen retrieval
    Human CXCL13/BLC/BCA‑1 Antibody AF801
  • Human CXCL13/BLC/BCA-1 Antibody
    Name: Chi P
    Application: Immunohistochemistry
    Sample Tested: Lymph node tissue
    Species: Rhesus Macaque
    Verified Customer | Posted 04/23/2018
    The CXCL13 can be observed in blue while CXCR5+ cells are observed in red
    Human CXCL13/BLC/BCA‑1 Antibody AF801
  • Human CXCL13/BLC/BCA-1 Antibody
    Name: Anonymous
    Application: Immunohistochemistry
    Sample Tested: Spleen tissue
    Species: Rhesus Macaque
    Verified Customer | Posted 03/21/2018
  • Name: Anonymous
    Application: Immunohistochemistry-Paraffin
    Sample Tested: See PMID 23778140
    Species: Human
    Verified Customer | Posted 01/09/2015

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