Human CXCL13/BLC/BCA‑1 Biotinylated Antibody

Name: Human CXCL13/BLC/BCA‑1 Biotinylated Antibody
Brand: R&D Systems
SKU: BAF801

Catalog # BAF801 | R&D Systems, Inc. a Bio-Techne Brand

Citations (3)

Product Datasheet / COA / SDS

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Product Summary
Applications
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Species Reactivity

Human

Applications

ELISA Detection (Matched Antibody Pair), Western Blot

Label

Biotin

Antibody Source

Polyclonal Goat IgG

Product Summary for Human CXCL13/BLC/BCA‑1 Biotinylated Antibody

Immunogen

E. coli-derived recombinant human CXCL13/BCA-1 (R&D Systems, Catalog # 801-CX)
Val23-Arg94
Accession # Q53X90

Specificity

Detects human BLC/BCA-1 in ELISAs and Western blots. In sandwich immunoassays, less than 0.05% cross-reactivity with recombinant human (rh) GRO alpha, rhGRO beta, rhGRO gamma, rhIL-8, rhIP-10, rhMIG, rhSDF-1 alpha, rmSDF‑1 alpha, and rhSDF-1 beta is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Applications for Human CXCL13/BLC/BCA‑1 Biotinylated Antibody

Application

Recommended Usage

Western Blot

0.1 µg/mL
Sample: Recombinant Human CXCL13/BLC/BCA‑1 (Catalog # 801-CX)

Human CXCL13/BLC/BCA-1 Sandwich Immunoassay

ELISA Detection (Matched Antibody Pair)

Recommended Concentration: 0.1-0.4 µg/mL
Use in combination with these reagents:

Detection Reagent: Human CXCL13/BLC/BCA‑1 Antibody (Catalog # MAB801)
Standard: Recombinant Human CXCL13/BLC/BCA-1 Protein (Catalog # 801-CX)

Please Note: Optimal dilutions of this antibody should be experimentally determined.

Published Applications

Read 3 publications using BAF801 in the following applications:

ELISA Development (1 Publication)
IHC-Fr (1 Publication)
IHC-P (1 Publication)

Formulation, Preparation and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CXCL13/BLC/BCA-1

CXCL13, also known as B-lymphocyte chemoattractant (BLC), is a CXC chemokine that is constitutively expressed in secondary lymphoid organs. BCA-1 cDNA encodes a protein of 109 amino acid residues with a leader sequence of 22 residues. Mature human BCA-1 shares 64% amino acid sequence similarity with the mouse protein and 23 - 34% amino acid sequence identity with other known CXC chemokines. Recombinant or chemically synthesized BCA-1 is a potent chemoattractant for B lymphocytes but not T lymphocytes, monocytes or neutrophils. BLR1, a G protein-coupled receptor originally isolated from Burkitt’s lymphoma cells, has now been shown to be the specific receptor for BCA-1. Among cells of the hematopoietic lineages, the expression of BLR1, now designated CXCR5, is restricted to B lymphocytes and a subpopulation of T helper memory cells. Mice lacking BLR1 have been shown to lack inguinal lymph nodes. These mice were also found to have impaired development of Peyer’s patches and defective formation of primary follicles and germinal centers in the spleen as a result of the inability of B lymphocytes to migrate into B cell areas.

References

Gunn, M.D. et al. (1998) Nature, 391:799.
Legler, D.F. et al. (1998) J. Exp. Med. 187:655.
Forster, R. et al. (1996) Cell 87:1037.

Alternate Names

ANGIE2, BCA-1, BCA1, BLC, BLR1L, SCYB13

Entrez Gene IDs

10563 (Human); 55985 (Mouse)

Gene Symbol

CXCL13

UniProt

Q53X90

Product Documents for Human CXCL13/BLC/BCA‑1 Biotinylated Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Human CXCL13/BLC/BCA‑1 Biotinylated Antibody

For research use only

Citations for Human CXCL13/BLC/BCA‑1 Biotinylated Antibody (3)

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Citations are publications that use Bio-Techne products. Selected citations for Human CXCL13/BLC/BCA‑1 Biotinylated Antibody include:

Species: Human
Sample Types: Whole Tissue
Applications: IHC-Fr

Hoorweg K et al. (2015-09-16), A Stromal Cell Niche for Human and Mouse Type 3 Innate Lymphoid Cells. J Immunol, 2015-0195(9):4257-63.
PMID: 26378073
Species: Human
Sample Types: Whole Tissue
Applications: IHC-P

Cagigi A et al. (2008-09-09), Altered expression of the receptor-ligand pair CXCR5/CXCL13 in B cells during chronic HIV-1 infection. Blood, 2008-0112(12):4401-10.
PMID: 18780835
Species: Human
Sample Types: Cell Culture Supernates
Applications: ELISA Development

Lisignoli G et al. (2006-05-01), Hyaluronan-based polymer scaffold modulates the expression of inflammatory and degradative factors in mesenchymal stem cells: Involvement of Cd44 and Cd54. J. Cell. Physiol., 2006-0207(2):364-73.
PMID: 16331675

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