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Human CXCL13/BLC/BCA‑1 Antibody Best Seller

Catalog # MAB801 | R&D Systems, Inc. a Bio-Techne Brand
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MAB801-100
MAB801-500
MAB801-SP

Key Product Details

Species Reactivity

Human

Applications

ELISA Capture (Matched Antibody Pair), Neutralization, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 53610

Product Summary for Human CXCL13/BLC/BCA‑1 Antibody

Immunogen

E. coli-derived recombinant human CXCL13/BLC/BCA-1
Val23-Arg94
Accession # O43927

Specificity

Detects human CXCL13/BLC/BCA-1 in ELISAs and Western blots. In ELISAs, no cross-reactivity with recombinant human (rh) CXCL1, rhCXCL2, rhCXCL3, rhCXCL8/IL‑8, rhCXCL10, rhCXCL9, rhCXCL12/SDF‑1 alpha, or rhCXCL12/SDF‑1 beta is observed. In Western blots, no cross‑reactivity with recombinant mouse CXCL13/BLC/BCA-1 is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human CXCL13/BLC/BCA‑1 Antibody

Detection of Recombinant Human CXCL13/BLC/BCA-1 antibody by Western Blot.

Detection of Recombinant Human CXCL13/BLC/BCA‑1 by Western Blot.

Western blot shows 100 ng of Recombinant Human CXCL13/BLC/BCA-1 (Catalog # 801-CX) and Recombinant Mouse CXCL13/BLC/BCA-1 (Catalog # 470-BC). PVDF Membrane was probed with 1 µg/mL of Mouse Anti-Human CXCL13/BLC/BCA-1 Monoclonal Antibody (Catalog # MAB801) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for CXCL13/BLC/BCA-1 at approximately 12 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3.
Chemotaxis Induced by CXCL13/BLC/BCA‑1 and Neutralization by Human CXCL13/BLC/BCA‑1 Antibody.

Chemotaxis Induced by CXCL13/BLC/BCA‑1 and Neutralization by Human CXCL13/BLC/BCA‑1 Antibody.

Recombinant Human CXCL13/BLC/BCA-1 (Catalog # 801-CX) chemoattracts the BaF3 mouse pro-B cell line transfected with human CXCR5 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Mouse Anti-Human CXCL13/BLC/BCA-1 (0.05 µg/mL) is neutralized (green line) by increasing concentrations of Human CXCL13/BLC/BCA-1 Monoclonal Antibody (Catalog # MAB801). The ND50 is typically 0.3-0.9 µg/mL.

Applications for Human CXCL13/BLC/BCA‑1 Antibody

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: Recombinant Human CXCL13/BLC/BCA‑1 (Catalog # 801-CX)

Neutralization

Measured by its ability to neutralize CXCL13/BLC/BCA‑1-induced chemotaxis in the BaF3 mouse pro‑B cell line transfected with human CXCR5. The Neutralization Dose (ND50) is typically 0.3-0.9 µg/mL in the presence of 0.05 µg/mL Recombinant Human CXCL13/BLC/BCA‑1.

Human CXCL13/BLC/BCA-1 Sandwich Immunoassay

ELISA Capture (Matched Antibody Pair)
Recommended Concentration: 2-8 µg/mL
Use in combination with these reagents:
  • Detection Reagent: Human CXCL13/BLC/BCA‑1 Biotinylated Antibody (Catalog # BAF801)
  • Standard: Recombinant Human CXCL13/BLC/BCA-1 Protein (Catalog # 801-CX)
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 3 reviews rated 4.7 using MAB801 in the following applications:

Published Applications

Read 12 publications using MAB801 in the following applications:

Formulation, Preparation and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CXCL13/BLC/BCA-1

CXCL13, also known as B-lymphocyte chemoattractant (BLC), is a CXC chemokine that is constitutively expressed in secondary lymphoid organs. BCA-1 cDNA encodes a protein of 109 amino acid residues with a leader sequence of 22 residues. Mature human BCA-1 shares 64% amino acid sequence similarity with the mouse protein and 23-34% amino acid sequence identity with other known CXC chemokines. Recombinant or chemically synthesized BCA-1 is a potent chemoattractant for B lymphocytes but not T lymphocytes, monocytes or neutrophils. BLR1, a G protein-coupled receptor originally isolated from Burkitt’s lymphoma cells, has now been shown to be the specific receptor for BCA-1. Among cells of the hematopoietic lineages, the expression of BLR1, now designated CXCR5, is restricted to B lymphocytes and a subpopulation of T helper memory cells. Mice lacking BLR1 have been shown to lack inguinal lymph nodes. These mice were also found to have impaired development of Peyer’s patches and defective formation of primary follicles and germinal centers in the spleen as a result of the inability of B lymphocytes to migrate into B cell areas.

References

  1. Gunn, M.D. et al. (1998) Nature, 391:799.
  2. Legler, D.F. et al. (1998) J. Exp. Med. 187:655.
  3. Forster, R. et al. (1996) Cell 87:1037.

Alternate Names

ANGIE2, BCA-1, BCA1, BLC, BLR1L, SCYB13

Entrez Gene IDs

10563 (Human); 55985 (Mouse)

Gene Symbol

CXCL13

UniProt

Product Documents for Human CXCL13/BLC/BCA‑1 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human CXCL13/BLC/BCA‑1 Antibody

For research use only

Citations for Human CXCL13/BLC/BCA‑1 Antibody (12)

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Customer Reviews for Human CXCL13/BLC/BCA‑1 Antibody (3)

4.7 out of 5
3 customer ratings
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  • Human CXCL13/BLC/BCA-1 Antibody
    Name: Anonymous
    Application: Immunohistochemistry
    Sample Tested: Tonsil tissue
    Species: Human
    Verified Customer | Posted 02/07/2020
    IHC-FFPE section pretreatment: heating in acidic buffer (BOND ER1, Leica) HIER in basic buffer is not suitable primary Ab: 10ug/mL (x50) diluted in Renoir Red Diluent (Biocare), incubation for 30min Detection:Leica Bond Refine polymer
    Human CXCL13/BLC/BCA‑1 Antibody MAB801
  • Human CXCL13/BLC/BCA-1 Antibody
    Name: Anonymous
    Application: Cell depletion
    Sample Tested: Peripheral blood mononuclear cells (PBMCs)
    Species: Human
    Verified Customer | Posted 04/23/2018
  • Human CXCL13/BLC/BCA-1 Antibody
    Name: Anonymous
    Application: Cell depletion
    Sample Tested: Cell Culture Supernates
    Species: Human
    Verified Customer | Posted 06/28/2016

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FAQs for Human CXCL13/BLC/BCA‑1 Antibody

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  • Q: What is the light chain of Human CXCL13/BLC/BCA-1 Antibody, Catalog # MAB801, Clone # 53610?

    A: Catalog # MAB801 has a kappa light chain.

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