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Human CXCL13/BLC/BCA‑1 Antibody Best Seller
Catalog # MAB801 | R&D Systems, Inc. a Bio-Techne Brand
Key Product Details
Species Reactivity
Human
Applications
ELISA Capture (Matched Antibody Pair), Neutralization, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # 53610
Product Summary for Human CXCL13/BLC/BCA‑1 Antibody
Immunogen
E. coli-derived recombinant human CXCL13/BLC/BCA-1
Val23-Arg94
Accession # O43927
Val23-Arg94
Accession # O43927
Specificity
Detects human CXCL13/BLC/BCA-1 in ELISAs and Western blots. In ELISAs, no cross-reactivity with recombinant human (rh) CXCL1, rhCXCL2, rhCXCL3, rhCXCL8/IL‑8, rhCXCL10, rhCXCL9, rhCXCL12/SDF‑1 alpha, or rhCXCL12/SDF‑1 beta is observed. In Western blots, no cross‑reactivity with recombinant mouse CXCL13/BLC/BCA-1 is observed.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for Human CXCL13/BLC/BCA‑1 Antibody
Detection of Recombinant Human CXCL13/BLC/BCA‑1 by Western Blot.
Western blot shows 100 ng of Recombinant Human CXCL13/BLC/BCA-1 (Catalog # 801-CX) and Recombinant Mouse CXCL13/BLC/BCA-1 (Catalog # 470-BC). PVDF Membrane was probed with 1 µg/mL of Mouse Anti-Human CXCL13/BLC/BCA-1 Monoclonal Antibody (Catalog # MAB801) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for CXCL13/BLC/BCA-1 at approximately 12 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3.
Chemotaxis Induced by CXCL13/BLC/BCA‑1 and Neutralization by Human CXCL13/BLC/BCA‑1 Antibody.
Recombinant Human CXCL13/BLC/BCA-1 (Catalog # 801-CX) chemoattracts the BaF3 mouse pro-B cell line transfected with human CXCR5 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Mouse Anti-Human CXCL13/BLC/BCA-1 (0.05 µg/mL) is neutralized (green line) by increasing concentrations of Human CXCL13/BLC/BCA-1 Monoclonal Antibody (Catalog # MAB801). The ND50 is typically 0.3-0.9 µg/mL.Applications for Human CXCL13/BLC/BCA‑1 Antibody
Application
Recommended Usage
Western Blot
1 µg/mL
Sample: Recombinant Human CXCL13/BLC/BCA‑1 (Catalog # 801-CX)
Sample: Recombinant Human CXCL13/BLC/BCA‑1 (Catalog # 801-CX)
Neutralization
Measured by its ability to neutralize CXCL13/BLC/BCA‑1-induced chemotaxis in the BaF3 mouse pro‑B cell line transfected with human CXCR5. The Neutralization Dose (ND50) is typically 0.3-0.9 µg/mL in the presence of 0.05 µg/mL Recombinant Human CXCL13/BLC/BCA‑1.
Human CXCL13/BLC/BCA-1 Sandwich Immunoassay
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Reviewed Applications
Read 3 reviews rated 4.7 using MAB801 in the following applications:
Published Applications
Read 12 publications using MAB801 in the following applications:
Formulation, Preparation and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: CXCL13/BLC/BCA-1
CXCL13, also known as B-lymphocyte chemoattractant (BLC), is a CXC chemokine that is constitutively expressed in secondary lymphoid organs. BCA-1 cDNA encodes a protein of 109 amino acid residues with a leader sequence of 22 residues. Mature human BCA-1 shares 64% amino acid sequence similarity with the mouse protein and 23-34% amino acid sequence identity with other known CXC chemokines. Recombinant or chemically synthesized BCA-1 is a potent chemoattractant for B lymphocytes but not T lymphocytes, monocytes or neutrophils. BLR1, a G protein-coupled receptor originally isolated from Burkitt’s lymphoma cells, has now been shown to be the specific receptor for BCA-1. Among cells of the hematopoietic lineages, the expression of BLR1, now designated CXCR5, is restricted to B lymphocytes and a subpopulation of T helper memory cells. Mice lacking BLR1 have been shown to lack inguinal lymph nodes. These mice were also found to have impaired development of Peyer’s patches and defective formation of primary follicles and germinal centers in the spleen as a result of the inability of B lymphocytes to migrate into B cell areas.
Product Documents for Human CXCL13/BLC/BCA‑1 Antibody
Product Specific Notices for Human CXCL13/BLC/BCA‑1 Antibody
For research use only
Citations for Human CXCL13/BLC/BCA‑1 Antibody (12)
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Citations are publications that use Bio-Techne products. Selected citations for Human CXCL13/BLC/BCA‑1 Antibody include:
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Species: Mouse
Sample Types: Whole Tissue
Applications: IHCSamson et al. (2023-08-24), Belatacept inhibit human B cell germinal center development in immunodeficient mice Scientific reports
PMID: 37620431 -
Species: Human
Sample Types: Whole Tissue
Applications: IHC-PA Satou et al. (2019-04-05), Methotrexate-associated lymphoproliferative disorders of T-cell phenotype: clinicopathological analysis of 28 cases Mod. Pathol., 2019-00(0).
PMID: 30952973 -
Species: Human
Sample Types: Whole Tissue
Applications: IHCR Muto et al. (2018-10-11), Epidemiology and secular trends of malignant lymphoma in Japan: Analysis of 9426 cases according to the World Health Organization classification Cancer Med, 2018-10(0).
PMID: 30311404 -
Species: Human
Sample Types: Whole Cells
Applications: Flow CytometryDS Thommen et al. (2018-06-11), A transcriptionally and functionally distinct PD-1+ CD8+ T cell pool with predictive potential in non-small-cell lung cancer treated with PD-1 blockade Nat. Med., 2018-00(0).
PMID: 29892065 -
Species: Human
Sample Types: Whole Tissue
Applications: IHC-PLA Aqrawi et al. (2018-03-24), Diminished CXCR5 expression in peripheral blood of patients with Sj�gren's syndrome may relate to both genotype and salivary gland homing Clin. Exp. Immunol., 2018-00(0).
PMID: 29453859 -
Species: Human
Sample Types: Whole Cells
Applications: ICCA Asai et al. (2017-03-03), Host antitumor resistance improved by the macrophage polarization in a chimera model of patients with HCC Oncoimmunology, 2017-06(4):e1299301.
PMID: 28507807 -
Species: Human
Sample Types: Recombinant Protein
Applications: Direct ELISAKlimatcheva E et al. (2015-02-12), CXCL13 antibody for the treatment of autoimmune disorders. BMC Immunol, 2015-016(0):6.
PMID: 25879435 -
Species: Human
Sample Types: CSF
Applications: Immuno-PCREdwards et al. (2013-11-20), Feasibility of the use of combinatorial chemokine arrays to study blood and CSF in multiple sclerosis. PLoS ONE, 2013-18(11):e81007.
PMID: 24278364 -
Species: Human
Sample Types: Cell Lysates,Whole Tissue
Applications: IHC-P,Western BlotPanse J et al. (2008-09-16), Chemokine CXCL13 is overexpressed in the tumour tissue and in the peripheral blood of breast cancer patients. Br. J. Cancer, 2008-099(6):930-8.
PMID: 18781150 -
Species: Human
Sample Types: Whole Tissue
Applications: IHC-PBurkle A et al. (2007-07-25), Overexpression of the CXCR5 chemokine receptor, and its ligand, CXCL13 in B-cell chronic lymphocytic leukemia. Blood, 2007-0110(9):3316-25.
PMID: 17652619 -
Species: Human
Sample Types: Cell Culture Supernates
Applications: ELISA DevelopmentLisignoli G et al. (2006-05-01), Hyaluronan-based polymer scaffold modulates the expression of inflammatory and degradative factors in mesenchymal stem cells: Involvement of Cd44 and Cd54. J. Cell. Physiol., 2006-0207(2):364-73.
PMID: 16331675 -
Species: Human
Sample Types: Whole Tissue
Applications: IHC-PLisignoli G et al. (2004-04-01), IL1beta and TNFalpha differently modulate CXCL13 chemokine in stromal cells and osteoblasts isolated from osteoarthritis patients: evidence of changes associated to cell maturation. Exp. Gerontol., 2004-039(4):659-65.
PMID: 15050303
There are no citations that match your criteria.
Customer Reviews for Human CXCL13/BLC/BCA‑1 Antibody (3)
4.7 out of 5
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Application: ImmunohistochemistrySample Tested: Tonsil tissueSpecies: HumanVerified Customer | Posted 02/07/2020IHC-FFPE section pretreatment: heating in acidic buffer (BOND ER1, Leica) HIER in basic buffer is not suitable primary Ab: 10ug/mL (x50) diluted in Renoir Red Diluent (Biocare), incubation for 30min Detection:Leica Bond Refine polymer
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Application: Cell depletionSample Tested: Peripheral blood mononuclear cells (PBMCs)Species: HumanVerified Customer | Posted 04/23/2018
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Application: Cell depletionSample Tested: Cell Culture SupernatesSpecies: HumanVerified Customer | Posted 06/28/2016
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