Human CTLA-4 (Research Grade Ipilimumab Biosimilar) Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB9928

Recombinant Monoclonal Antibody For Research Use Only
R&D Systems, part of Bio-Techne

Key Product Details

Species Reactivity

Human

Applications

Flow Cytometry, CyTOF-ready

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Human IgG1 Clone # Hu111

View all CTLA-4 Antibodies »

Product Specifications

Immunogen

Human CTLA-4

Specificity

Detects Human CTLA-4 based on Ipilimumab therapeutic antibody.This non-therapeutic antibody uses the same variable region sequence as the therapeutic antibody Ipilimumab. This product is for research use only.

Clonality

Monoclonal

Host

Human

Isotype

IgG1

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Scientific Data Images for Human CTLA-4 (Research Grade Ipilimumab Biosimilar) Antibody

Detection of CTLA-4 antibody in HEK293 Human cell line Transfected with Human CTLA-4 and eGFP antibody by Flow Cytometry.

Detection of CTLA-4 in HEK293 Human cell line Transfected with Human CTLA-4 and eGFP by Flow Cytometry.

HEK293 Human cell line transfected with (A) Human CTLA-4 or (B) irrelevant transfectants and eGFP was stained with Human Anti-Human CTLA-4 (Research Grade Ipilimumab) Monoclonal Antibody (Catalog # MAB9928) followed by APC-conjugated Anti-Human IgG Secondary Antibody (Catalog # F0135). View our protocol for Staining Membrane-associated Proteins.

Applications for Human CTLA-4 (Research Grade Ipilimumab Biosimilar) Antibody

Application
Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

0.25 µg/106 cells
Sample: HEK293 Human cell line Transfected with Human CTLA-4 and eGFP

Formulation, Preparation, and Storage

Purification

Protein A or G purified from cell culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
Size / Price
Qty

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CTLA-4

Ipilimumab is a biosimilar directed against the extracellular domain of CTLA‑4. This product constitutes the immunogen used for the anti-Idiotype catalog number MAB10064. The biotinylated antibody (catalog number FAB9928B) can be used in flow cytometry or various assay format to measure CTLA-4 expression and can be detected by conjugated streptavidin.

Long Name

Cytotoxic T-lymphocyte-associated Molecule 4

Alternate Names

CD152, CTLA4

Entrez Gene IDs

1493 (Human); 12477 (Mouse); 403696 (Canine); 102115124 (Cynomolgus Monkey)

Gene Symbol

CTLA4

Additional CTLA-4 Products

Product Documents for Human CTLA-4 (Research Grade Ipilimumab Biosimilar) Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human CTLA-4 (Research Grade Ipilimumab Biosimilar) Antibody

For research use only

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