Human IgG APC-conjugated Antibody

Name: Human IgG APC-conjugated Antibody
Brand: R&D Systems
SKU: F0135
Rating: 5 (1 reviews)

R&D Systems, Inc. a Bio-Techne Brand

Catalog # F0135

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Key Product Details

Species Reactivity:	Human
Immunogen:	Human IgG
Applications:	Flow Cytometry
Label:	Allophycocyanin (Excitation=620-650 nm, Emission=660-670 nm)
Source:	Polyclonal Goat IgG
Specificity:	This antibody reacts with heavy chains on human IgG and with light chains on all human immunoglobulins. No reactivity is observed to non-immunoglobulin human, mouse, rabbit or bovine serum proteins by IEP.

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Applications for Human IgG APC-conjugated Antibody

	Recommended Concentration	Sample
Flow Cytometry	Designed for use as a secondary developing reagent in immunofluorescent assays, such as flow cytometry, where the primary antibody does not have a fluorescent reporter, is of human origin, and is of IgG class. The recommended concentration is 10 μL/10⁶cells.

Please Note: Optimal dilutions should be determined by each laboratory for each application.

Scientific Data Examples for Human IgG APC-conjugated Antibody

	Flow Cytometry Detection of EGF R/ErbB1 in A431 human epithelial carcinoma cell line by Flow Cytometry. A431 human epithelial carcinoma cell line was stained with Human Anti-Human EGF R/ErbB1 (Cetuximab) Monoclonal Antibody (Catalog # MAB9577, filled histogram) or irrelevant antibody (open histogram) followed by APC-conjugated Anti-Human IgG Secondary Antibody (Catalog #F0135). View our protocol for Staining Membrane-associated Proteins.

Formulation, Preparation and Storage

Purification	Antigen Affinity-purified
Formulation	Supplied in a saline solution containing BSA and Sodium Azide.
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied.

Plan Your Experiments

Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

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Background: IgG

R&D Systems offers a range of secondary antibodies and controls for flow cytometry, immunohistochemistry, and Western blotting. We provide species-specific secondary antibodies that are available with a variety of conjugated labels.

Our NorthernLights fluorescent secondary antibodies are bright and resistant to photobleaching. We are currently offering secondary antibodies recognizing mouse, rat, goat, sheep, and rabbit IgG as well as chicken IgY. These reagents are available with three distinct excitation and emission maxima, making them ideal for multi-color fluorescence microscopy.

Item	Value
Long Name	Immunoglobulin G
Alternate Names	IgG, Immunoglobulin G, ImmunoglobulinG

Citations for Human IgG APC-conjugated Antibody

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Customer Reviews for Human IgG APC-conjugated Antibody (1)

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Human IgG APC-conjugated Antibody

Name: Anonymous

Application: Flow

Sample Tested: Peripheral blood mononuclear cells (PBMCs)

Species: Canine

Verified Customer | Posted 06/26/2020

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Product Documents for Human IgG APC-conjugated Antibody

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Human IgG APC-conjugated Antibody

R&D Systems, Inc. a Bio-Techne Brand

Key Product Details

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Applications for Human IgG APC-conjugated Antibody

Scientific Data Examples for Human IgG APC-conjugated Antibody

Flow Cytometry

Formulation, Preparation and Storage

Plan Your Experiments

Reconstitution Calculator

Background: IgG

Citations for Human IgG APC-conjugated Antibody

Customer Reviews for Human IgG APC-conjugated Antibody (1)

Customer Images

Human IgG APC-conjugated Antibody

Product Documents for Human IgG APC-conjugated Antibody

Product Datasheets

COA

Certificate of Analysis

SDS

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