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Human Claudin‑6 Antibody

Catalog # MAB3656 | R&D Systems, Inc. a Bio-Techne Brand
Catalog #
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Key Product Details

Species Reactivity



CyTOF-ready, Flow Cytometry, Immunocytochemistry



Antibody Source

Monoclonal Mouse IgG2B Clone # 342927

Product Summary for Human Claudin‑6 Antibody


NS0 mouse myeloma cell line transfected with human Claudin-6
Accession # P56747


Stains human Claudin-6 transfected cells but not irrelevant transfectants.







Scientific Data Images for Human Claudin‑6 Antibody

Detection of Claudin-6 antibody in HEK293 Human Cell Line Transfected with Human Claudin-6 and eGFP antibody by Flow Cytometry.

Detection of Claudin-6 in HEK293 Human Cell Line Transfected with Human Claudin-6 and eGFP by Flow Cytometry.

HEK293 human cell line transfected with (A) Human Claudin-6 or (B) irrelevant protein, and eGFP was stained with Mouse Anti-Human Claudin-6 Monoclonal Antibody (Catalog # MAB3656) followed by APC-conjugated Goat anti-Mouse IgG Secondary Antibody (Catalog # F0101B). Quadrant markers were set based on Mouse IgG2B Isotype Control Antibody (Catalog # MAB0041). View our protocol for Staining Membrane-associated Proteins.
Claudin-6 antibody in iPS2 Human Induced Pluripotent Stem Cells by Immunocytochemistry (ICC).

Claudin‑6 in iPS2 Human Induced Pluripotent Stem Cells.

Claudin-6 was detected in immersion fixed iPS2 human induced pluripotent stem cells, differentiated into definitive endoderm with StemXVivo Endoderm Kit (Catalog # SC019), using Mouse Anti-Human Claudin-6 Monoclonal Antibody (Catalog # MAB3656) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). SOX17 was also detected using Goat Anti-Human SOX17 Antigen Affinity-purified Polyclonal Antibody (green; Catalog # AF1924). Specific staining of Claudin-6 was localized to cell surfaces. View our protocol for Fluorescent ICC Staining of Stem Cells on Coverslips.

Applications for Human Claudin‑6 Antibody

Recommended Usage


Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

0.25 µg/106 cells
Sample: HEK293 Human Cell Line Transfected with Human Claudin-6 and eGFP


8-25 µg/mL
Sample: Immersion fixed iPS2 human induced pluripotent stem cells differentiated into definitive endoderm using StemXVivo Endoderm Kit (Catalog # SC019)
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation and Storage


Protein A or G purified from hybridoma culture supernatant


Reconstitute at 0.5 mg/mL in sterile PBS.

Reconstitution Buffer Available:
Size / Price


Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.


The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Claudin-6

Claudin-6 is a multipass transmembrane protein in the Claudin family. Claudin-6 is expressed by epithelial cells where it participates in tissue development and the maintenance of tight junction integrity. Human Claudin-6 shares 88% and 86% amino acid sequence identity with mouse and rat Claudin-6, respectively.

Alternate Names

Claudin6, CLDN6, Skullin 2

Entrez Gene IDs

9074 (Human); 54419 (Mouse); 287098 (Rat)

Gene Symbol



Product Documents for Human Claudin‑6 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human Claudin‑6 Antibody

For research use only

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