Human Claudin‑6 Antibody

Name: Human Claudin‑6 Antibody
Brand: R&D Systems
SKU: MAB3656
Price: 171 EUR

Catalog # MAB3656 | R&D Systems, Inc. a Bio-Techne Brand

Citations (2)

Product Datasheet / COA / SDS

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Product Summary
Scientific Data
Applications
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Species Reactivity

Human

Applications

CyTOF-ready, Flow Cytometry, Immunocytochemistry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG_2B Clone # 342927

Product Summary for Human Claudin‑6 Antibody

Immunogen

NS0 mouse myeloma cell line transfected with human Claudin-6
Met1-Val220
Accession # P56747

Specificity

Stains human Claudin-6 transfected cells but not irrelevant transfectants.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG_2B

Scientific Data Images for Human Claudin‑6 Antibody

Detection of Claudin-6 in HEK293 Human Cell Line Transfected with Human Claudin-6 and eGFP by Flow Cytometry.

HEK293 human cell line transfected with (A) Human Claudin-6 or (B) irrelevant protein, and eGFP was stained with Mouse Anti-Human Claudin-6 Monoclonal Antibody (Catalog # MAB3656) followed by APC-conjugated Goat anti-Mouse IgG Secondary Antibody (Catalog # F0101B). Quadrant markers were set based on Mouse IgG2B Isotype Control Antibody (Catalog # MAB0041). View our protocol for Staining Membrane-associated Proteins.

Claudin‑6 in iPS2 Human Induced Pluripotent Stem Cells.

Claudin-6 was detected in immersion fixed iPS2 human induced pluripotent stem cells, differentiated into definitive endoderm with StemXVivo Endoderm Kit (Catalog # SC019), using Mouse Anti-Human Claudin-6 Monoclonal Antibody (Catalog # MAB3656) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). SOX17 was also detected using Goat Anti-Human SOX17 Antigen Affinity-purified Polyclonal Antibody (green; Catalog # AF1924). Specific staining of Claudin-6 was localized to cell surfaces. View our protocol for Fluorescent ICC Staining of Stem Cells on Coverslips.

Applications for Human Claudin‑6 Antibody

Application

Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

0.25 µg/10⁶ cells
Sample: HEK293 Human Cell Line Transfected with Human Claudin-6 and eGFP

Immunocytochemistry

8-25 µg/mL
Sample: Immersion fixed iPS2 human induced pluripotent stem cells differentiated into definitive endoderm using StemXVivo Endoderm Kit (Catalog # SC019)

Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Claudin-6

Claudin-6 is a multipass transmembrane protein in the Claudin family. Claudin-6 is expressed by epithelial cells where it participates in tissue development and the maintenance of tight junction integrity. Human Claudin-6 shares 88% and 86% amino acid sequence identity with mouse and rat Claudin-6, respectively.

Alternate Names

Claudin6, CLDN6, Skullin 2

Entrez Gene IDs

9074 (Human); 54419 (Mouse); 287098 (Rat)

Gene Symbol

CLDN6

UniProt

P56747

Product Documents for Human Claudin‑6 Antibody

Product Datasheets

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COA

SDS

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Product Specific Notices for Human Claudin‑6 Antibody

For research use only

Citations for Human Claudin‑6 Antibody (2)

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Citations are publications that use Bio-Techne products. Selected citations for Human Claudin‑6 Antibody include:

Species: Human
Applications: Bioassay

Y Shirasago et al. (2022-11-24), A single mutation in the E2 glycoprotein of hepatitis C virus broadens the claudin specificity for its infection Scientific Reports, 2022-112(1):20243.
PMID: 36424447
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry,Functional Assay

J Haddad et al. (2017-03-29), Identification of novel functions for hepatitis C virus envelope glycoprotein E1 in virus entry and assembly J. Virol, 2017-00(0).
PMID: 28179528

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