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Human SOX17 Antibody Best Seller

Bio-Techne includes R&D Systems | Catalog # AF1924

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AF1924
AF1924-SP

Key Product Details

Species Reactivity

Validated:

Human

Cited:

Mouse, Rat, Bovine, Callithrix jacchus (Marmoset), Canine, Marmoset, Pan troglodytes (Chimpanzee), Primate - Macaca fascicularis (Crab-eating Monkey or Cynomolgus Macaque), Primate - Macaca mulatta (Rhesus Macaque), Transgenic Mouse, Xenograft

Applications

Chromatin Immunoprecipitation (ChIP), Immunocytochemistry, Simple Western, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Product Summary for Human SOX17 Antibody

Immunogen

E. coli-derived recombinant human SOX17
Asp177-Val414
Accession # Q9H6I2

Specificity

Detects human SOX17 in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human (rh) SOX18 is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human SOX17 Antibody

Detection of Human SOX17 antibody by Western Blot.

Detection of Human SOX17 by Western Blot.

Western blot shows lysates of BG01V human embryonic stem cells untreated (-) or endoderm differentiated (+). PVDF membrane was probed with 1 µg/mL of Goat Anti-Human SOX17 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1924) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (HAF109). A specific band was detected for SOX17 at approximately 55 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of SOX17-regulated Genes antibody by Chromatin Immunoprecipitation.

Detection of SOX17-regulated Genes by Chromatin Immunoprecipitation.

Endoderm-differentiated D3 mouse embryonic stem cell line was fixed using formaldehyde, resuspended in lysis buffer, and sonicated to shear chromatin. SOX17/DNA complexes were immunoprecipitated using 5 µg Goat Anti-Human SOX17 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1924) or control antibody (AB-108-C) for 15 minutes in an ultrasonic bath, followed by Biotinylated Anti-Goat IgG Secondary Antibody (BAF109). Immunocomplexes were captured using 50 µL of MagCellect Streptavidin Ferrofluid (MAG999) and DNA was purified using chelating resin solution. Thep21promoter was detected by standard PCR.
SOX17 antibody in B16 Mouse Cell Line by Immunocytochemistry (ICC).

SOX17 in B16 Mouse Cell Line.

SOX17 was detected in immersion fixed B16 mouse melanoma cell line using 10 µg/mL Goat Anti-Human SOX17 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1924) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; NL001) and counter-stained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Applications for Human SOX17 Antibody

Application
Recommended Usage

Chromatin Immunoprecipitation (ChIP)

5 µg/5 x 106 cells
Sample: Endoderm-differentiated D3 mouse embryonic stem cell line chromatin, p21 promoter detected by standard PCR.

Immunocytochemistry

5-15 µg/mL
Sample: Immersion fixed B16 mouse melanoma cell line and immersion fixed endoderm differentiated BG01V human embryonic stem cells

Simple Western

10 µg/mL
Sample: Endoderm differentiated BG01V human embryonic stem cells and endoderm differentiated iBJ6 human induced pluripotent stem cell line

Western Blot

1-2 µg/mL
Sample: Endoderm differentiated BG01V human embryonic stem cells, SK‑OV‑3 human ovarian adenocarcinoma cell line and OVCAR‑3  human ovarian carcinoma cell line
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 17 reviews rated 4.8 using AF1924 in the following applications:

Published Applications

Read 449 publications using AF1924 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: SOX17

SOX17 is a member of the SOX family of transcription factors that bind DNA via a high mobility group (HMG) domain. SOX17 is suggested to play an important role in endoderm development (1, 2).

References

  1. Kanai-Azuma, M. et al. (2002) Development 129:2367.
  2. Katoh, M. et al. (2002) Int. J. Mol. Med. 9:153.

Long Name

Transcription Factor SOX17

Alternate Names

FLJ22252, SRY (sex determining region Y)-box 17, SRY-related HMG-box transcription factor SOX17, transcription factor SOX-17, VUR3

Entrez Gene IDs

64321 (Human)

Gene Symbol

SOX17

UniProt

Additional SOX17 Products

Product Documents for Human SOX17 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human SOX17 Antibody

For research use only

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