Human cIAP-1/HIAP-2 Antibody

Name: Human cIAP-1/HIAP-2 Antibody
Brand: R&D Systems
SKU: AF8181
Rating: 4 (4 reviews)

R&D Systems, Inc. a Bio-Techne Brand

Catalog # AF8181

Citations (43)

(4)

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Key Product Details

Species Reactivity:	Human
Immunogen:	E. coli-derived recombinant human cIAP-1/HIAP-2 His2-Ser618 Accession # Q13490
Applications:	Immunohistochemistry, Knockout Validated, Simple Western, Western Blot
Label:	Unconjugated
Source:	Polyclonal Goat IgG
Specificity:	Detects human cIAP-1/HIAP-2. Does not cross-react with recombinant human cIAP-2 or XIAP.

View Full Technical Details for Human cIAP-1/HIAP-2 Antibody

Applications for Human cIAP-1/HIAP-2 Antibody

	Recommended Concentration	Sample
Western Blot	0.5 µg/mL	See below
Simple Western	5 µg/mL	See below
Immunohistochemistry	5-15 µg/mL	See below

Item	Value
Knockout Validated	cIAP‑1/HIAP‑2 is specifically detected in HeLa human cervical epithelial carcinoma parental cell line but is not detectable in cIAP‑1/HIAP‑2 knockout HeLa cell line.

Please Note: Optimal dilutions should be determined by each laboratory for each application.

Scientific Data Examples for Human cIAP-1/HIAP-2 Antibody

	Western Blot Detection of Human cIAP‑1/HIAP‑2 by Western Blot. Western blot shows lysates of HEK293 human embryonic kidney cell line transfected with human cIAP-1 (lane 1), human cIAP-2 (lane 2), or non-transfected (lane 3). PVDF membrane was probed with 0.5 µg/mL Goat Anti-Human cIAP-1/HIAP-2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8181) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). For additional reference, lystates of HepG2 human hepatocellular carcinoma cell line and Jurkat human acute T cell leukemia cell line were included. A specific band for cIAP-1/HIAP-2 was detected at approximately 65 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.
	Immunohistochemistry cIAP‑1/HIAP‑2 in Human Lymph Node. cIAP-1/HIAP-2 was detected in immersion fixed paraffin-embedded sections of human lymph node using Goat Anti-Human cIAP-1/HIAP-2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8181) at 10 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to lymphocytes. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
	Immunohistochemistry cIAP‑1/HIAP‑2 in Human Lymphoma. cIAP-1/HIAP-2 was detected in immersion fixed paraffin-embedded sections of human lymphoma using Goat Anti-Human cIAP-1/HIAP-2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8181) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
	Simple Western Detection of Human cIAP‑1/HIAP‑2 by Simple Western^TM. Simple Western lane view shows lysates of HepG2 human hepatocellular carcinoma cell line, loaded at 0.5 mg/mL. A specific band was detected for cIAP-1/HIAP-2 at approximately 66 kDa (as indicated) using 5 µg/mL of Goat Anti-Human cIAP-1/HIAP-2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8181) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
	Knockout Validated Western Blot Shows Human cIAP‑1/HIAP‑2 Specificity by Using Knockout Cell Line. Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and cIAP-1/HIAP-2 knockout HeLa cell line (KO). PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human cIAP-1/HIAP-2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8181) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for cIAP-1/HIAP-2 at approximately 68 kDa (as indicated) in the parental HeLa cell line, but is not detectable in knockout HeLa cell line. GAPDH (Catalog # AF5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
	Knockout Validated Specificity of Human cIAP‑1/HIAP‑2 by Simple Western^TM. Simple Western lane view shows lysates of HeLa human cervical epithelial carcinoma parental cell line and cIAP-1/HIAP-2 knockout HeLa cell line (KO), loaded at 0.2 mg/mL. A specific band was detected for cIAP-1/HIAP-2 at approximately 66 kDa (as indicated) in the parental HeLa cell line, but is not detectable in knockout HeLa cell line. Goat Anti-Human cIAP-1/HIAP-2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8181) was used at 5 µg/mL followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Formulation, Preparation and Storage

Purification	Antigen Affinity-purified
Reconstitution	Reconstitute at 0.2 mg/mL in sterile PBS. Reconstitution Buffer Available: Size / Price Qty Loading... RB01:
Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.

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Background: cIAP-1/HIAP-2

cIAP-1 (also known as BIR2, MIHB and HIAP-2) is a member of the inhibitor of apoptosis (IAP) family of proteins that inhibit the proteolytic activity of mature caspases. cIAP-1 has 3 BIR (baculovirus inhibitor of apoptosis) domains, a RING finger domain, and a caspase recruitment domain (CARD). cIAP-1 inhibits caspases by interaction of the BIR domain with the active caspase. Caspase activity may be restored through interactions with the Reaper like motif on mitochondrial proteins such as SMAC/Diablo or HTRA-2/Omi. cIAP-1 is reported to be cleaved by caspases in fetal rat hepatocytes treated with TGF-beta.

Item	Value
References	Roy, N. et al. (1997) EMBO J. 23:6914. Deveraux, Q. et al. (1997) Nature 388:300. Deveraux, Q. and J. Reed (1999) Genes & Develop. 13:239. Herrera, B. et al. (2002) FEBS Letters 520:93.
Long Name	Cellular Inhibitor of Apoptosis Protein 1
Entrez Gene IDs	329 (Human); 11797 (Mouse)
Alternate Names	API1Hiap-2, BIRC2, HIAP-2, IAP homolog B, IAP-2, IAP2, Inhibitor of apoptosis protein 2, MIHB, MIHBHIAP2, NFR2-TRAF signalling complex protein, RING finger protein 48, RNF48hiap-2, TNFR2-TRAF-signaling complex protein 2, apoptosis inhibitor 1, baculoviral IAP repeat containing 2, baculoviral IAP repeat-containing 2, baculoviral IAP repeat-containing protein 2, c-IAP1, cIAP-1, cIAP1, hIAP2

Citations for Human cIAP-1/HIAP-2 Antibody (43)

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Citations are publications that use Bio-Techne products. Selected citations for Human cIAP-1/HIAP-2 Antibody include:

Species: Human
Sample Types: Cell Lysates
Applications: Immunoprecipitation

Y Akizuki et al. (2022), cIAP1-based degraders induce degradation via branched ubiquitin architectures Nature Chemical Biology, 0(0).
PMID: 36316570
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot

Y An et al. (2021), Death agonist antibody against TRAILR2/DR5/TNFRSF10B enhances birinapant anti-tumor activity in HPV-positive head and neck squamous cell carcinomas Scientific Reports, 11(1):6392.
PMID: 33737574
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot

V Granqvist et al. (2021), Induction of interferon-&beta and interferon signaling by TRAIL and Smac mimetics via caspase-8 in breast cancer cells PLoS ONE, 16(3):e0248175.
PMID: 33770100
Species: Human
Sample Types: Whole Tissue
Applications: IHC

DE Muench et al. (2020), Mouse models of neutropenia reveal progenitor-stage-specific defects Nature, 582(7810):109-114.
PMID: 32494068
Species: Human
Sample Types: Whole Tissue
Applications: IHC

CC Nixon et al. (2020), Systemic HIV and SIV latency reversal via non-canonical NF-kappaB signalling in vivo Nature, 578(7793):160-165.
PMID: 31969707
Species: Human
Sample Types: Cell Lysates
Applications: Immunoprecipitation

S Lee et al. (2019), Absence of Cytosolic 2-Cys Prx Subtypes I and II Exacerbates TNF-?-Induced Apoptosis via Different Routes Cell Rep, 26(8):2194-2211.e6.
PMID: 30784599
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot

LGT Lemos et al. (2019), The LQB-223 Compound Modulates Antiapoptotic Proteins and Impairs Breast Cancer Cell Growth and Migration Int J Mol Sci, 20(20).
PMID: 31614718
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot

J Dittmann et al. (2019), Next-generation hypomethylating agent SGI-110 primes acute myeloid leukemia cells to IAP antagonist by activating extrinsic and intrinsic apoptosis pathways Cell Death Differ., 0(0).
PMID: 31831875
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot

N Ohoka et al. (2018), Derivatization of inhibitor of apoptosis protein (IAP) ligands yields improved inducers of estrogen receptor ? degradation J. Biol. Chem., 0(0).
PMID: 29545311
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot

GA Ward et al. (2018), ASTX660, a novel non-peptidomimetic antagonist of cIAP1/2 and XIAP, potently induces TNF-? dependent apoptosis in cancer cell lines and inhibits tumor growth Mol. Cancer Ther., 0(0).
PMID: 29695633
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot

N Shibata et al. (2018), Pharmacological difference between degrader and inhibitor against oncogenic BCR-ABL kinase Sci Rep, 8(1):13549.
PMID: 30202081
Species: Human
Sample Types: Cell Lysates
Applications: Immunoprecipitation

J Allègre et al. (2018), E2F1 binds to the peptide-binding groove within the BIR3 domain of cIAP1 and requires cIAP1 for chromatin binding PLoS ONE, 13(10):e0206253.
PMID: 30359437
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot

V Glorian et al. (2017), DNA damage and S phase-dependent E2F1 stabilization requires the cIAP1 E3-ubiquitin ligase and is associated with K63-poly-ubiquitination on lysine 161/164 residues Cell Death Dis, 8(5):e2816.
PMID: 28542143
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot

A Mohr et al. (2017), Caspase-10: a molecular switch from cell-autonomous apoptosis to communal cell death in response to chemotherapeutic drug treatment Cell Death Differ., 0(0).
PMID: 29099485
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot

V Liapis et al. (2017), Anticancer efficacy of the hypoxia-activated prodrug evofosfamide is enhanced in combination with proapoptotic receptor agonists against osteosarcoma Cancer Med, 0(0).
PMID: 28799237
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot

S Biswas et al. (2017), Molluscum Contagiosum Virus MC159 abrogates cIAP1-NEMO interactions and inhibits NEMO polyubiquitination J. Virol., 0(0).
PMID: 28515292
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot

N Shibata et al. (2017), Development of protein degradation inducers of oncogenic BCR-ABL protein by conjugation of ABL kinase inhibitors and IAP ligands Cancer Sci., 108(8):1657-1666.
PMID: 28556300
Species: Human
Sample Types: Tissue Homogenates
Applications: Western Blot

ME Guicciardi et al. (2017), Biliary tract instillation of a SMAC mimetic induces TRAIL-dependent acute sclerosing cholangitis-like injury in mice Cell Death Dis, 8(1):e2535.
PMID: 28055006
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot

H Takaki et al. (2017), Hepatitis C Virus Infection Increases c-Jun N-Terminal Kinase (JNK) Phosphorylation and Accentuates Hepatocyte Lipoapoptosis Med. Sci. Monit., 23(0):4526-4532.
PMID: 28931802
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot

V Glorian et al. (2017), DNA damage and S phase-dependent E2F1 stabilization requires the cIAP1 E3-ubiquitin ligase and is associated with K63-poly-ubiquitination on lysine 161/164 residues Cell Death Dis, 8(5):e2816.
PMID: 28542143
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot

L Zhao et al. (2017), NCTD promotes Birinapant-mediated anticancer activity in breast cancer cells by downregulation of c-FLIP Oncotarget, 8(16):26886-26895.
PMID: 28460471
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot

Keiichiro Okuhira (2016), Targeted degradation of proteins localized in subcellular compartments by hybrid small molecules Mol. Pharmacol, 0(0).
PMID: 27965304
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot

Schoeneberger H et al. (2015), Impairment of antioxidant defense via glutathione depletion sensitizes acute lymphoblastic leukemia cells for Smac mimetic-induced cell death. Oncogene, 34(31):4032-43.
PMID: 25381820
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot

L Hernandez et al. (2015), A dual role for Caspase8 and NF-?B interactions in regulating apoptosis and necroptosis of ovarian cancer, with correlation to patient survival Cell Death Discov, 1(0):15053.
PMID: 28179987
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot

Zinonos I et al. (2014), Doxorubicin overcomes resistance to drozitumab by antagonizing Inhibitor of Apoptosis Proteins (IAPs). Anticancer Res, 34(12):7007-20.
PMID: 25503127
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot

Qin Q et al. (2014), Small-molecule survivin inhibitor YM155 enhances radiosensitization in esophageal squamous cell carcinoma by the abrogation of G2 checkpoint and suppression of homologous recombination repair. J Hematol Oncol, 7(0):62.
PMID: 25139395
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry

Smolewski P et al. (2014), Cytotoxic activity of the amphibian ribonucleases onconase and r-amphinase on tumor cells from B cell lymphoproliferative disorders. Int J Oncol, 45(1):419-25.
PMID: 24789756
Species: Human
Sample Types: Cell Lysates
Applications: Immunoprecipitation

Wang Q et al. (2014), Receptor-interacting protein 1 increases chemoresistance by maintaining inhibitor of apoptosis protein levels and reducing reactive oxygen species through a microRNA-146a-mediated catalase pathway. J Biol Chem, 289(9):5654-63.
PMID: 24425875
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot

Guicciardi et al. (2014), Cellular inhibitor of apoptosis (cIAP)-mediated ubiquitination of phosphofurin acidic cluster sorting protein 2 (PACS-2) negatively regulates tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) cytotoxicity. PLoS ONE, 9(3):e92124.
PMID: 24633224
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot

Uno M et al. (2014), NF-kappaB inducing kinase, a central signaling component of the non-canonical pathway of NF-kappaB, contributes to ovarian cancer progression. PLoS ONE, 9(2):e88347.
PMID: 24533079
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot

Wagner L et al. (2013), Smac mimetic sensitizes glioblastoma cells to Temozolomide-induced apoptosis in a RIP1- and NF-kappaB-dependent manner. Oncogene, 32(8):988-97.
PMID: 22469979
Species: Human
Sample Types: Whole Cells
Applications: Western Blot

Hornburger M et al. (2013), A novel role for inhibitor of apoptosis (IAP) proteins as regulators of endothelial barrier function by mediating RhoA activation. FASEB J, 28(4):1938-46.
PMID: 24371121
Species: Human
Sample Types: Tissue Homogenates
Applications: Western Blot

Krepler C et al. (2013), The novel SMAC mimetic birinapant exhibits potent activity against human melanoma cells. Clin Cancer Res, 19(7):1784-94.
PMID: 23403634
Species: Human
Sample Types: Cell Lysates
Applications: Flow Cytometry

(2012), Role of Apollon in human melanoma resistance to antitumor agents that activate the intrinsic or the extrinsic apoptosis pathways. Clin. Cancer Res., 18(12):3316-27.
PMID: 22553342
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot

Oberoi-Khanuja et al. (2012), Role of melanoma inhibitor of apoptosis (ML-IAP) protein, a member of the baculoviral IAP repeat (BIR) domain family, in the regulation of C-RAF kinase and cell migration. J Biol Chem, 287(34):28445-55.
PMID: 22711539
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot

Engesaeter B et al. (2012), Dacarbazine and the agonistic TRAIL receptor-2 antibody lexatumumab induce synergistic anticancer effects in melanoma. PLoS ONE, 7(9):e45492.
PMID: 23029050
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot

Varfolomeev et al. (2012), Characterization of ML-IAP protein stability and physiological role in vivo. Biochem J, 447(3):427-36.
PMID: 22853455
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot

Ikner A et al. (2011), TWEAK Induces Apoptosis through a Death-signaling Complex Comprising Receptor-interacting Protein 1 (RIP1), Fas-associated Death Domain (FADD), and Caspase-8. J. Biol. Chem., 286(24):21546-54.
PMID: 21525013
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot

Loder S et al. (2011), RIP1 is required for IAP inhibitor-mediated sensitization of childhood acute leukemia cells to chemotherapy-induced apoptosis. Leukemia, 26(5):1020-9.
PMID: 22173242
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot

Reddy RM et al. (2007), Rapid and profound potentiation of Apo2L/TRAIL-mediated cytotoxicity and apoptosis in thoracic cancer cells by the histone deacetylase inhibitor Trichostatin A: the essential role of the mitochondria-mediated caspase activation cascade. Apoptosis, 12(1):55-71.
PMID: 17136498
Species: Feline
Sample Types: Tissue Homogenates
Applications: Western Blot

Balasubramanian S et al. (2006), Enhanced ubiquitination of cytoskeletal proteins in pressure overloaded myocardium is accompanied by changes in specific E3 ligases. J. Mol. Cell. Cardiol., 41(4):669-79.
PMID: 16928382
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot

Samuel T et al. (2005), cIAP1 Localizes to the nuclear compartment and modulates the cell cycle. Cancer Res., 65(1):210-8.
PMID: 15665297
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot

Mitsiades CS et al. (2002), Activation of NF-kappaB and upregulation of intracellular anti-apoptotic proteins via the IGF-1/Akt signaling in human multiple myeloma cells: therapeutic implications. Oncogene, 21(37):5673-83.
PMID: 12173037

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Customer Reviews for Human cIAP-1/HIAP-2 Antibody (4)

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Human cIAP-1/HIAP-2 Antibody

Name: Anonymous

Application: Microarrays

Sample Tested: EDTA Plasma

Species: Human

Verified Customer | Posted 01/03/2020
Human cIAP-1/HIAP-2 Antibody

Name: Anonymous

Application: MiAr

Sample Tested: EDTA Plasma

Species: Human

Verified Customer | Posted 01/09/2019
Human cIAP-1/HIAP-2 Antibody

Name: Anonymous

Application: ELISA

Sample Tested: Plasma

Species: Human

Verified Customer | Posted 11/10/2018
Human cIAP-1/HIAP-2 Antibody

Name: Anonymous

Application: WB

Sample Tested: MCF-7 human breast cancer cell line

Species: Human

Verified Customer | Posted 03/27/2017

Expression of hcIAP-1/HIAP-2 in MCF-7 cells untreated (A) and after treatment with 10 uM Paclitaxel for 4 hours (B) using Goat anti-Human cIAP-1/HIAP-2 antibody (#AF8181) at 0.5 ug/mL, followed by Donkey anti-Goat secondary antibody at 1:5,000 dilution.

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Human cIAP-1/HIAP-2 Antibody

R&D Systems, Inc. a Bio-Techne Brand

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Applications for Human cIAP-1/HIAP-2 Antibody

Scientific Data Examples for Human cIAP-1/HIAP-2 Antibody

Western Blot

Immunohistochemistry

Immunohistochemistry

Simple Western

Knockout Validated

Knockout Validated

Formulation, Preparation and Storage

Reconstitution Calculator

Background: cIAP-1/HIAP-2

Citations for Human cIAP-1/HIAP-2 Antibody (43)

Customer Reviews for Human cIAP-1/HIAP-2 Antibody (4)

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Human cIAP-1/HIAP-2 Antibody

Human cIAP-1/HIAP-2 Antibody

Human cIAP-1/HIAP-2 Antibody

Human cIAP-1/HIAP-2 Antibody

Product Documents for Human cIAP-1/HIAP-2 Antibody

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