Human CEACAM-18 Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB9869

R&D Systems, part of Bio-Techne

Key Product Details

Species Reactivity

Human

Applications

Flow Cytometry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 1057633

View all CEACAM-18 Antibodies »

Product Specifications

Immunogen

Human embryonic kidney cell, HEK293-derived human CEACAM-18
Gln31-His317
Accession # A8MTB9

Specificity

Detects human CEACAM-18 in direct ELISA.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human CEACAM-18 Antibody

Detection of CEACAM-18 in HEK293 cells by Flow Cytometry.

HEK293 cells transfected with Human CEACAM-18 (A) vs Irrelevant transfectant cells (B) were stained with Mouse Anti-Human CEACAM-18 Monoclonal Antibody (Catalog # MAB9869, filled histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B). View our protocol for Staining Membrane-associated Proteins.

Applications for Human CEACAM-18 Antibody

Application
Recommended Usage

Flow Cytometry

0.25 µg/106 cells
Sample: HEK293 cells transfected with Human CEACAM-18 vs Irrelevant transfectant

Formulation, Preparation, and Storage

Purification

Protein A or G purified from cell culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
Size / Price
Qty

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CEACAM-18

Carcinoembryonic Antigen-related Cell Adhesion Molecule 18 (CEACAM-18) is part of the CEA protein family consisting of CEACAMs and the pregnancy-specific glycoproteins (PSGs). Both CEACAM and PSG molecules have been identified in humans and belong to the much larger glycosylphosphatidylinositol (GPI)-linked immunoglobulin (Ig) superfamily (1, 2). Mature human CEACAM-18 has a 298 amino acid (aa) extracellular domain containing 2 IgC2-like and 1 IgV-like domains, a single transmembrane domain and a short cytoplasmic tail (2). CEACAM-18 is one of only five conserved CEACAMs among mouse, rat, and human (2), but mature human CEACAM-18 has low aa sequence identify with mouse and rat at 60% and 58%, respectively. Originally discovered as a biomarker for colorectal cancer (3), CEACAMs have now been associated with numerous intracellular signaling processes including cell adhesion, cell growth, recognition and differentiation, angiogenesis, and apoptosis (4-6). While the exact function of CEACAM-18 has been yet to be elucidated, it may bind pathogen receptors or other immunoregulatory members (6). CEACAM family members were identified as the major Galectin-3 receptor candidates on human neutrophils (7). Binding of carbohydrate ligands to CEACAMs may be important in the release of proinflammatory mediators (8, 9).

References

  1. Beauchemin, N. et al. (1999) Exp. Cell Res. 252:243.
  2. Zebhauser, R. et al. (2005) Genomics 86:566.
  3. Gold, P and Freedman SO, 1965) J Exp Med 122:467.
  4. Obrink, B. (1997) Curr Opin Cell Biol 9:616.
  5. Horst, A.K. and Wagener, C. (2004) Handb Exp Pharmacol 283.
  6. Kuespert, K. et al. (2006) Curr Opin Cell Biol. 18(5):565.
  7. Feuk-Lagerstedt, E. et al. (1999) J. Immunol. 163:5592.
  8. Yoon, J. et al. (2007) J. Immunol. 179:8454.
  9. Schröder, A.K. et al. (2006) Hum Immunol. 67:676.

Long Name

Carcinoembryonic Antigen-related Cell Adhesion Molecule 18

Alternate Names

Carcinoembryonic Antigen-Related Cell Adhesion Molecule 18

Entrez Gene IDs

729767 (Human); 72431 (Mouse); 502334 (Rat)

Gene Symbol

CEACAM18

UniProt

A8MTB9

Additional CEACAM-18 Products

Product Documents for Human CEACAM-18 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Human CEACAM-18 Antibody

For research use only

Privacy and Cookie Policy
Site Map
© Copyright 2024 Bio-Techne. All Rights Reserved.