Human CEACAM-18 Alexa Fluor® 647-conjugated Antibody

R&D Systems, part of Bio-Techne | Catalog # FAB9869R

R&D Systems, part of Bio-Techne

Key Product Details

Species Reactivity

Human

Applications

Flow Cytometry

Label

Alexa Fluor 647 (Excitation = 650 nm, Emission = 668 nm)

Antibody Source

Monoclonal Mouse IgG1 Clone # 1057633

View all CEACAM-18 Antibodies »

Product Specifications

Immunogen

Human embryonic kidney cell, HEK293-derived human CEACAM-18
Gln31-His317
Accession # A8MTB9

Specificity

Detects human CEACAM-18 in direct ELISA.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Applications for Human CEACAM-18 Alexa Fluor® 647-conjugated Antibody

Application
Recommended Usage

Flow Cytometry

Titration recommended for optimal concentration with starting range of 0.1-1 µg/1 million cells. Sample used for this experiment was HEK293 cells transfected with Human CEACAM-18 vs Irrelevant transfectant.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from cell culture supernatant

Formulation

Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: CEACAM-18

Carcinoembryonic Antigen-related Cell Adhesion Molecule 18 (CEACAM-18) is part of the CEA protein family consisting of CEACAMs and the pregnancy-specific glycoproteins (PSGs). Both CEACAM and PSG molecules have been identified in humans and belong to the much larger glycosylphosphatidylinositol (GPI)-linked immunoglobulin (Ig) superfamily (1, 2). Mature human CEACAM-18 has a 298 amino acid (aa) extracellular domain containing 2 IgC2-like and 1 IgV-like domains, a single transmembrane domain and a short cytoplasmic tail (2). CEACAM-18 is one of only five conserved CEACAMs among mouse, rat, and human (2), but mature human CEACAM-18 has low aa sequence identify with mouse and rat at 60% and 58%, respectively. Originally discovered as a biomarker for colorectal cancer (3), CEACAMs have now been associated with numerous intracellular signaling processes including cell adhesion, cell growth, recognition and differentiation, angiogenesis, and apoptosis (4-6). While the exact function of CEACAM-18 has been yet to be elucidated, it may bind pathogen receptors or other immunoregulatory members (6). CEACAM family members were identified as the major Galectin-3 receptor candidates on human neutrophils (7). Binding of carbohydrate ligands to CEACAMs may be important in the release of proinflammatory mediators (8, 9).

References

  1. Beauchemin, N. et al. (1999) Exp. Cell Res. 252:243.
  2. Zebhauser, R. et al. (2005) Genomics 86:566.
  3. Gold, P and Freedman SO, 1965) J Exp Med 122:467.
  4. Obrink, B. (1997) Curr Opin Cell Biol 9:616.
  5. Horst, A.K. and Wagener, C. (2004) Handb Exp Pharmacol 283.
  6. Kuespert, K. et al. (2006) Curr Opin Cell Biol. 18(5):565.
  7. Feuk-Lagerstedt, E. et al. (1999) J. Immunol. 163:5592.
  8. Yoon, J. et al. (2007) J. Immunol. 179:8454.
  9. Schröder, A.K. et al. (2006) Hum Immunol. 67:676.

Long Name

Carcinoembryonic Antigen-related Cell Adhesion Molecule 18

Alternate Names

Carcinoembryonic Antigen-Related Cell Adhesion Molecule 18

Entrez Gene IDs

729767 (Human); 72431 (Mouse); 502334 (Rat)

Gene Symbol

CEACAM18

UniProt

A8MTB9

Additional CEACAM-18 Products

Product Documents

Product Datasheets

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human CEACAM-18 Alexa Fluor® 647-conjugated Antibody


This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.

For research use only

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