Human CD4 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB114341
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human
Applications
Flow Cytometry
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2B Clone # 1068606
Product Specifications
Immunogen
Chinese Hamster Ovary cell line, CHO-derived human CD4
Gln26-Lys463
Accession # P08575
Gln26-Lys463
Accession # P08575
Specificity
Detects human CD4 in direct ELISA.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Scientific Data Images for Human CD4 Antibody
Detection of CD4 in PBMC lymphocytes stained with CD4 RPA-T4 by Flow Cytometry.
PBMC lymphocytes were stained with CD4 RPA-T4 (MAB37912). Cells were washed and stained with (A) Mouse Anti-Human CD4 Monoclonal Antibody (Catalog #MAB114341) or (B) Mouse IgG2B Isotype Control (MAB004) followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (F0102B) and Mouse Anti-Human CD3 epsilon APC conjugated Monoclonal Antibody (FAB100A). This demonstrates non-competing epitopes of MAB114341 and MAB37912 which allows for purity analysis after CD4+ selection. View our protocol for Staining Membrane-associated Proteins.
Detection of CD4 in Human peripheral blood lymphocytes by Flow Cytometry
Human peripheral blood lymphocytes were stained with Mouse Anti-Human CD4 Monoclonal Antibody (Catalog # MAB114341) which was PE-conjugated (FAB114341P), and Mouse Anti-Human CD4 Alexa Fluor® 647-conjugated Monoclonal Antibody (FAB37912R). This demonstrates non-competing epitopes of the two clones, which allows for purity analysis after CD4+ selection. View our protocol for Staining Membrane-associated Proteins.Applications for Human CD4 Antibody
Application
Recommended Usage
Flow Cytometry
0.25 µg/106 cells
Sample: PBMC Lymphocytes
Sample: PBMC Lymphocytes
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: CD4
CD4 is an approximately 55 kDa type I membrane glycoprotein that is expressed predominantly on most thymocytes and a subset of mature T lymphocytes. In humans, CD4 is also expressed to a lesser extent on monocytes and macrophage related cells. Human CD4 cDNA encodes a 458 amino acid (aa) precursor protein with a 25 aa signal peptide, a 371 aa extracellular region containing four immunoglobulin homology domains, a 24 aa transmembrane domain and a 38 aa cytoplasmic domain. CD4 is a coreceptor required for T cell recognition of antigens that are presented by class II major histocompatibility complexes. CD4 has been shown to be a coreceptor of HIV entry and specifically binds gp120, the external envelope glycoprotein of HIV.
References
- Capon, D.I. et al. (1991) Annu. Rev. Immunol. 9:649.
Alternate Names
CD4
Entrez Gene IDs
Gene Symbol
CD4
UniProt
Additional CD4 Products
Product Documents for Human CD4 Antibody
Product Specific Notices for Human CD4 Antibody
For research use only
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