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Key Product Details

Species Reactivity

Human

Applications

CyTOF-ready, Flow Cytometry

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Mouse IgG1 Clone # CTC5R

Product Summary for Human CCR5 Antibody

Immunogen

CHO Chinese hamster ovary cell line transfected with human CCR5

Specificity

Detects human CCR5 in direct Flow cytometry.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human CCR5 Antibody

Detection of CCR5 antibody in Human Peripheral Blood Lymphocytes antibody by Flow Cytometry.

Detection of CCR5 in Human Peripheral Blood Lymphocytes by Flow Cytometry.

Human peripheral blood lymphocytes were stained with (A) Mouse Anti-Human CCR5 Monoclonal Antibody (Catalog # MAB1802R) or (B) control antibody (Catalog # MAB002), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B) and Mouse anti-Human CD3 APC-conjugated monoclonal antibody (Catalog # FAB100A). View our protocol for Staining Membrane-associated Proteins.

Applications for Human CCR5 Antibody

Application
Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

0.25 µg/106 cells
Sample: Human peripheral blood lymphocytes
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CCR5

CCR5 is a G protein-linked seven transmembrane domain chemokine receptor. CCR5 serves as a receptor for several chemokines including MIP-1 alpha, MIP-1 beta, MCP-2, and RANTES. It also functions as a coreceptor for Macrophage Tropic HIV-1 infection.

References

  1. Lee, B. et al. (1999) J. Biol. Chem. 274:9617.

Alternate Names

CCR5, CD195

Entrez Gene IDs

1234 (Human); 12774 (Mouse); 117029 (Rat); 484789 (Canine); 493769 (Feline)

Gene Symbol

CCR5

Additional CCR5 Products

Product Documents for Human CCR5 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human CCR5 Antibody

For research use only

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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