Human CCL4/MIP-1 beta MAb (Clone 24006)
R&D Systems, part of Bio-Techne | Catalog # MAB271
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Conjugate
Catalog #
Key Product Details
Validated by
Biological Validation
Species Reactivity
Validated:
Human
Cited:
Human, Primate - Macaca fascicularis (Crab-eating Monkey or Cynomolgus Macaque)
Applications
Validated:
Western Blot, ELISA Capture (Matched Antibody Pair), Neutralization, Intracellular Staining by Flow Cytometry, CyTOF-ready
Cited:
Immunohistochemistry, Immunohistochemistry-Frozen, Western Blot, Neutralization, Flow Cytometry, Immunocytochemistry, Antibody Array Development, ELISA Development, ELISpot Development, Immunodepletion, Lateral Flow Assay, Luminex Development, CyTOF-reported
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2B Clone # 24006
Product Specifications
Immunogen
S. frugiperda insect ovarian cell line Sf 21-derived recombinant human CCL4/MIP-1 beta
Ala24-Asn92
Accession # P13236
Ala24-Asn92
Accession # P13236
Specificity
Detects human CCL4/MIP-1 beta in ELISAs and Western blots. In Western blots, less than 4% cross-reactivity with recombinant human(rh) CCL3/MIP‑1 alpha and no cross-reactivity with recombinant mouse (rm) CCL3/MIP‑1 alpha, rmCCL4/MIP‑1 beta, rhCXCL8/IL‑8, rhCCL5, rhCXCL1, rhCXCL2, or rhCXCL3 is observed.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Scientific Data Images for Human CCL4/MIP-1 beta MAb (Clone 24006)
Chemotaxis Induced by CCL4/MIP-1 beta and Neutralization by Human CCL4/MIP-1 beta Antibody.
Recombinant Human CCL4/MIP-1 beta (Catalog # 271-BME) chemoattracts the BaF3 mouse pro-B cell line transfected with human CCR5 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Human CCL4/MIP-1 beta (0.04 µg/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human CCL4/MIP-1 beta Monoclonal Antibody (Catalog # MAB271). The ND50 is typically 0.3-1.2 µg/mL.
Detection of CCL4/MIP‑1 beta in Human Blood Monocytes by Flow Cytometry.
Human peripheral blood monoctyes treated with LPS and Monensin for 5 hours were stained with Mouse Anti-Human CD14 APC-conjugated Monoclonal Antibody (Catalog # FAB3832A) and either (A) Mouse Anti-Human CCL4/MIP-1 beta Monoclonal Antibody (Catalog # MAB271) or (B) Mouse IgG2BFlow Cytometry Isotype Control (Catalog # MAB0041) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.
Human CCL4 / MIP-1 beta ELISA Standard Curve
Recombinant Human CCL4/MIP-1 beta (Catalog # 271-BME) was serially diluted and captured by Mouse Anti-Human CCL4/MIP-1 beta Monoclonal Antibody (Catalog # MAB271) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human CCL4/MIP-1 beta Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-271-NA) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)Applications for Human CCL4/MIP-1 beta MAb (Clone 24006)
Application
Recommended Usage
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Intracellular Staining by Flow Cytometry
0.25 µg/106 cells
Sample: Human peripheral blood monoctyes treated with LPS and Monensin were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005)
Sample: Human peripheral blood monoctyes treated with LPS and Monensin were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005)
Western Blot
1 µg/mL
Sample: Recombinant Human CCL4/MIP-1 beta (Catalog # 271-BME)
Sample: Recombinant Human CCL4/MIP-1 beta (Catalog # 271-BME)
Neutralization
Measured by its ability to neutralize CCL4/MIP-1 beta-induced chemotaxis in the BaF3 mouse pro-B cell line transfected with human CCR5. The Neutralization Dose (ND50) is typically 0.3-1.2 µg/mL in the presence of 0.04 µg/mL Recombinant Human CCL4/MIP-1 beta.
Human CCL4/MIP-1 beta Sandwich Immunoassay
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Reviewed Applications
Read 5 reviews rated 5 using MAB271 in the following applications:
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: CCL4/MIP-1 beta
Alternate Names
Exodus-3, MIP-1 beta, MIP1 beta
Entrez Gene IDs
Gene Symbol
CCL4
UniProt
Additional CCL4/MIP-1 beta Products
Product Documents for Human CCL4/MIP-1 beta MAb (Clone 24006)
Product Specific Notices for Human CCL4/MIP-1 beta MAb (Clone 24006)
For research use only
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