Human Carbonic Anhydrase IX/CA9 Antibody

Name: Human Carbonic Anhydrase IX/CA9 Antibody
Brand: R&D Systems
SKU: MAB2188

R&D Systems, Inc. a Bio-Techne Brand

Catalog # MAB2188

Citations (3)

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Species Reactivity:	Human
Immunogen:	Mouse myeloma cell line NS0-derived recombinant human Carbonic Anhydrase IX Pro59-Asp414 Accession # Q16790
Applications:	CyTOF-ready, ELISA Capture, Flow Cytometry, Immunoprecipitation
Label:	Unconjugated
Source:	Monoclonal Mouse IgG_2A Clone # 303123
Specificity:	Detects human Carbonic Anhydrase IX (CA9) in direct ELISAs. In direct ELISAs, this antibody does not cross-react with recombinant mouse (rm) CA9 or with rhCA1, 2, 3, 4, 5A, 6, 7, 8, 10, 12, 13, or 14.

View Full Technical Details for Human Carbonic Anhydrase IX/CA9 Antibody

Applications for Human Carbonic Anhydrase IX/CA9 Antibody

	Recommended Concentration	Sample
Flow Cytometry	2.5 µg/10⁶ cells	HeLa human cervical epithelial carcinoma cell line
Immunoprecipitation	25 µg/mL	Conditioned cell culture medium spiked with Recombinant Human Carbonic Anhydrase IX (Catalog # 2188-CA), see our available Western blot detection antibodies
CyTOF-ready	Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Human Carbonic Anhydrase IX Sandwich Immunoassay

Recommended Concentration

Reagent

ELISA Capture (Matched Antibody Pair)

2-8 µg/mL

Use in combination with:

Detection Reagent: Human Carbonic Anhydrase IX Biotinylated Antibody (Catalog # BAF2188)

Standard: Recombinant Human Carbonic Anhydrase IX Protein, CF (Catalog # 2188-CA)

Please Note: Optimal dilutions should be determined by each laboratory for each application.

Formulation, Preparation and Storage

Purification	Protein A or G purified from hybridoma culture supernatant
Reconstitution	Reconstitute at 0.5 mg/mL in sterile PBS. Reconstitution Buffer Available: Size / Price Qty Loading... RB01:
Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.

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Background: Carbonic Anhydrase IX/CA9

Carbonic Anhydrase (CA) catalyzes the reversible reaction of CO₂ + H₂O = HCO₃^- + H⁺, which is fundamental to many processes such as respiration, renal tubular acidification and bone resorption (1-3). Topics in the CA meeting (6^th International Conference on the CAs, June 20-25, 2003; Slovakia) ranged from use of CAs as markers for tumor and hypoxia in clinic, as nutritional supplement in milk, and as a tool for CO₂ removal and mosquito control in industry. CA9, also known as membrane antigen MN and renal cell carcinoma (RCC)-associated antigen G250, is a transmembrane enzyme expressed primarily in carcinoma cells. It is one of the best markers for hypoxia and for RCC (4, 5). rhCA9 corresponds to the extracellular portion of human CA9.

Item	Value
References	Pastorek, J. et al. (1994) Oncogene 9:2877. Opavsky, R. et al. (1996) Genomics 33:480. Hewett-Emmett, D. and R.E. Tashian (1996) Mol. Phylogenet. Evol. 5:50. Kaluzova, M. et al. (2004) Mol. Cell Biol. 24:5757. Mukouyama, H. et al. (2004) Clin. Cancer Res. 10:1421.
Entrez Gene IDs	768 (Human); 230099 (Mouse)
Alternate Names	CA-IX, CA9, CAIX, Carbonate dehydratase IX, Carbonic Anhydrase IX, EC 4.2.1.1, G250, MN, Membrane antigen MN, P54/58N, PMW1, RCC, RCC-associated antigen G250, RCC-associated protein G250, Renal cell carcinoma-associated antigen G250, carbonic anhydrase 9, carbonic dehydratase

Citations for Human Carbonic Anhydrase IX/CA9 Antibody (3)

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Citations are publications that use Bio-Techne products. Selected citations for Human Carbonic Anhydrase IX/CA9 Antibody include:

Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry

R Alföldi et al. (2019), Single Cell Mass Cytometry of Non-Small Cell Lung Cancer Cells Reveals Complexity of In vivo And Three-Dimensional Models over the Petri-dish Cells, 8(9).
PMID: 31527554
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry

M Swayampaku et al. (2017), The interactome of metabolic enzyme carbonic anhydrase IX reveals novel roles in tumor cell migration and invadopodia/MMP14-mediated invasion Oncogene, 36(45):6244-6261.
PMID: 28692057
Species: Human
Sample Types: Whole Cells
Applications: ICC

Tan X et al. (2008), Global analysis of metastasis-associated gene expression in primary cultures from clinical specimens of clear-cell renal-cell carcinoma. Int. J. Cancer, 123(5):1080-8.
PMID: 18546293

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Human Carbonic Anhydrase IX/CA9 Antibody

R&D Systems, Inc. a Bio-Techne Brand

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