Human Carbonic Anhydrase IX/CA9 Alexa Fluor® 700-conjugated Antibody

Name: Human Carbonic Anhydrase IX/CA9 Alexa Fluor® 700-conjugated Antibody
Brand: R&D Systems
SKU: FAB2188N

R&D Systems, Inc. a Bio-Techne Brand

Catalog # FAB2188N

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Key Product Details

Species Reactivity:	Human
Immunogen:	Mouse myeloma cell line NS0-derived recombinant human Carbonic Anhydrase IX Pro59-Asp414 Accession # Q16790
Applications:	Flow Cytometry
Label:	Alexa Fluor 700 (Excitation=675-700 nm, Emission=723 nm)
Source:	Monoclonal Mouse IgG_2A Clone # 303123
Specificity:	Detects human Carbonic Anhydrase IX (CA9) in direct ELISAs. In direct ELISAs, this antibody does not cross-react with recombinant mouse (rm) CA9 or with rhCA1, 2, 3, 4, 5A, 6, 7, 8, 10, 12, 13, or 14.

View Full Technical Details for Human Carbonic Anhydrase IX/CA9 Alexa Fluor® 700-conjugated Antibody

Applications

	Recommended Concentration	Sample
Flow Cytometry	0.25-1 µg/10⁶ cells	U-87 MG human glioblastoma/astrocytoma cell line

Please Note: Optimal dilutions should be determined by each laboratory for each application.

Scientific Data Examples

	Flow Cytometry Detection of Carbonic Anhydrase IX/CA9 in U‑87 MG Human Cell Line by Flow Cytometry. U-87 MG human glioblastoma/astrocytoma cell line was stained with Mouse Anti-Human Carbonic Anhydrase IX/CA9 Alexa Fluor® 700-conjugated Monoclonal Antibody (Catalog # FAB2188N, filled histogram) or isotype control antibody (IC003N, open histogram). View our protocol for Staining Membrane-associated Proteins.

Formulation, Preparation and Storage

Purification	Protein A or G purified from hybridoma culture supernatant
Formulation	Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied.

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Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

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Background: Carbonic Anhydrase IX/CA9

Carbonic Anhydrase (CA) catalyzes the reversible reaction of CO₂ + H₂O = HCO₃^- + H⁺, which is fundamental to many processes such as respiration, renal tubular acidification and bone resorption (1-3). Topics in the CA meeting (6^th International Conference on the CAs, June 20-25, 2003; Slovakia) ranged from use of CAs as markers for tumor and hypoxia in clinic, as nutritional supplement in milk, and as a tool for CO₂ removal and mosquito control in industry. CA9, also known as membrane antigen MN and renal cell carcinoma (RCC)-associated antigen G250, is a transmembrane enzyme expressed primarily in carcinoma cells. It is one of the best markers for hypoxia and for RCC (4, 5). rhCA9 corresponds to the extracellular portion of human CA9.

Item	Value
References	Pastorek, J. et al. (1994) Oncogene 9:2877. Opavsky, R. et al. (1996) Genomics 33:480. Hewett-Emmett, D. and R.E. Tashian (1996) Mol. Phylogenet. Evol. 5:50. Kaluzova, M. et al. (2004) Mol. Cell Biol. 24:5757. Mukouyama, H. et al. (2004) Clin. Cancer Res. 10:1421.
Entrez Gene IDs	768 (Human); 230099 (Mouse)
Alternate Names	CA-IX, CA9, CAIX, Carbonate dehydratase IX, Carbonic Anhydrase IX, EC 4.2.1.1, G250, MN, Membrane antigen MN, P54/58N, PMW1, RCC, RCC-associated antigen G250, RCC-associated protein G250, Renal cell carcinoma-associated antigen G250, carbonic anhydrase 9, carbonic dehydratase

Product Specific Notices

This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.

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Human Carbonic Anhydrase IX/CA9 Alexa Fluor® 700-conjugated Antibody

R&D Systems, Inc. a Bio-Techne Brand

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Flow Cytometry

Formulation, Preparation and Storage

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Background: Carbonic Anhydrase IX/CA9

Product Specific Notices

Citations for Human Carbonic Anhydrase IX/CA9 Alexa Fluor® 700-conjugated Antibody

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