Human Carbonic Anhydrase IX/CA9 APC-conjugated Antibody

Name: Human Carbonic Anhydrase IX/CA9 APC-conjugated Antibody
Brand: R&D Systems
SKU: FAB2188A
Rating: 5 (1 reviews)

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Catalog # FAB2188A

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Species Reactivity:	Human
Immunogen:	Mouse myeloma cell line NS0-derived recombinant human Carbonic Anhydrase IX Pro59-Asp414 Accession # Q16790
Applications:	Flow Cytometry
Label:	Allophycocyanin (Excitation=620-650 nm, Emission=660-670 nm)
Source:	Monoclonal Mouse IgG_2A Clone # 303123
Specificity:	Detects human Carbonic Anhydrase IX (CA9) in direct ELISAs. In direct ELISAs, this antibody does not cross-react with recombinant mouse (rm) CA9 or with rhCA1, 2, 3, 4, 5A, 6, 7, 8, 10, 12, 13, or 14.

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Applications for Human Carbonic Anhydrase IX/CA9 APC-conjugated Antibody

	Recommended Concentration	Sample
Flow Cytometry	10 µL/10⁶ cells	See below

Please Note: Optimal dilutions should be determined by each laboratory for each application.

Scientific Data Examples for Human Carbonic Anhydrase IX/CA9 APC-conjugated Antibody

	Flow Cytometry Detection of Carbonic Anhydrase IX/CA9 in U‑87 MG Human Cell Line by Flow Cytometry. U-87 MG human glioblastoma/ astrocytoma cell line was stained with Mouse Anti-Human Carbonic Anhydrase IX/CA9 APC-conjugated Monoclonal Antibody (Catalog # FAB2188A, filled histogram) or isotype control antibody (Catalog # IC003A, open histogram). View our protocol for Staining Membrane-associated Proteins.

Formulation, Preparation and Storage

Purification	Protein A or G purified from hybridoma culture supernatant
Formulation	Supplied in a saline solution containing BSA and Sodium Azide.
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied.

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Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

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Background: Carbonic Anhydrase IX/CA9

Carbonic Anhydrase (CA) catalyzes the reversible reaction of CO₂ + H₂O = HCO₃^- + H⁺, which is fundamental to many processes such as respiration, renal tubular acidification and bone resorption (1-3). Topics in the CA meeting (6^th International Conference on the CAs, June 20-25, 2003; Slovakia) ranged from use of CAs as markers for tumor and hypoxia in clinic, as nutritional supplement in milk, and as a tool for CO₂ removal and mosquito control in industry. CA9, also known as membrane antigen MN and renal cell carcinoma (RCC)-associated antigen G250, is a transmembrane enzyme expressed primarily in carcinoma cells. It is one of the best markers for hypoxia and for RCC (4, 5). rhCA9 corresponds to the extracellular portion of human CA9.

Item	Value
References	Pastorek, J. et al. (1994) Oncogene 9:2877. Opavsky, R. et al. (1996) Genomics 33:480. Hewett-Emmett, D. and R.E. Tashian (1996) Mol. Phylogenet. Evol. 5:50. Kaluzova, M. et al. (2004) Mol. Cell Biol. 24:5757. Mukouyama, H. et al. (2004) Clin. Cancer Res. 10:1421.
Entrez Gene IDs	768 (Human); 230099 (Mouse)
Alternate Names	CA-IX, CA9, CAIX, Carbonate dehydratase IX, Carbonic Anhydrase IX, EC 4.2.1.1, G250, MN, Membrane antigen MN, P54/58N, PMW1, RCC, RCC-associated antigen G250, RCC-associated protein G250, Renal cell carcinoma-associated antigen G250, carbonic anhydrase 9, carbonic dehydratase

Citations for Human Carbonic Anhydrase IX/CA9 APC-conjugated Antibody

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Human Carbonic Anhydrase IX/CA9 APC-conjugated Antibody

Name: Anonymous

Application: Flow

Sample Tested: SK-OV-3 human ovarian adenocarcinoma cell line

Species: Human

Verified Customer | Posted 06/15/2018

SK-OV3 cells (negative for CA IX expression) and SK-OV3 transfected with human CA IX were stained analyzed by flow cytometry

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Human Carbonic Anhydrase IX/CA9 APC-conjugated Antibody

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Applications for Human Carbonic Anhydrase IX/CA9 APC-conjugated Antibody

Scientific Data Examples for Human Carbonic Anhydrase IX/CA9 APC-conjugated Antibody

Flow Cytometry

Formulation, Preparation and Storage

Plan Your Experiments

Reconstitution Calculator

Background: Carbonic Anhydrase IX/CA9

Citations for Human Carbonic Anhydrase IX/CA9 APC-conjugated Antibody

Customer Reviews for Human Carbonic Anhydrase IX/CA9 APC-conjugated Antibody (1)

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Human Carbonic Anhydrase IX/CA9 APC-conjugated Antibody

Product Documents for Human Carbonic Anhydrase IX/CA9 APC-conjugated Antibody

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