Human/Canine Integrin beta1/CD29 Antibody
R&D Systems, part of Bio-Techne | Catalog # AF1778
Scientific images may be resized, cropped, or adjusted for brightness or contrast for presentation purposes. These updates are limited to presentation and do not affect the underlying scientific interpretation of the data. Where available, additional source data may be provided upon request.
Conjugate
Catalog #
Key Product Details
Validated by
Knockout/Knockdown
Species Reactivity
Validated:
Human, Canine
Cited:
Human, Mouse
Applications
Validated:
Knockout Validated, Western Blot, Flow Cytometry, Immunocytochemistry, Simple Western, CyTOF-ready
Cited:
Western Blot, Neutralization, Immunocytochemistry, Functional Assay
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
Chinese hamster ovary cell line CHO-derived recombinant human Integrin beta1 isoform 1A
Gln21-Asp728
Accession # P05556
Gln21-Asp728
Accession # P05556
Specificity
Detects human Integrin beta1/CD29 in direct ELISAs and Western blots.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Human/Canine Integrin beta1/CD29 Antibody
Detection of Human Integrin beta1/CD29 by Western Blot.
Western blot shows lysates of MG-63 human osteosarcoma cell line, HeLa human cervical epithelial carcinoma cell line, A431 human epithelial carcinoma cell line, Jurkat human acute T cell leukemia cell line, and U-87 MG human glioblastoma/astrocytoma cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human/Canine Integrin beta1/CD29 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1778) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). Specific bands were detected for Integrin beta1/CD29 at approximately 130-140 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of Human Integrin beta1/CD29 by Simple WesternTM.
Simple Western shows lysates of Exosome Standards (HT-29) (NBP3-11685) and Jurkat human acute T cell leukemia cell line, loaded at 0.5 mg/ml. A specific band was detected for Integrin beta1/CD29 at approximately 198 kDa (as indicated) using 20 µg/mL of Goat Anti-Human/Canine Integrin beta1/CD29 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1778). This experiment was conducted under reducing conditions and using the 12-230kDa separation system.
Detection of Integrin beta1/CD29 in Canine PBMCs by Flow Cytometry.
Canine peripheral blood mononuclear cells (PBMCs) were stained with Goat Anti-Human/Canine Integrin beta1/CD29 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1778, filled histogram) or isotype control antibody (Catalog # AB-108-C, open histogram), followed by Phycoerythrin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0107).
Applications for Human/Canine Integrin beta1/CD29 Antibody
Application
Recommended Usage
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Flow Cytometry
0.25 µg/106 cells
Sample: Human peripheral blood mononuclear cells (PBMCs), canine peripheral blood mononuclear cells (PBMCs), and canine mesenchymal stem cells
Sample: Human peripheral blood mononuclear cells (PBMCs), canine peripheral blood mononuclear cells (PBMCs), and canine mesenchymal stem cells
Immunocytochemistry
5-15 µg/mL
Sample: Immersion fixed canine mesenchymal stem cells, human mesenchymal stem cells, and A549 human lung carcinoma cell line
Sample: Immersion fixed canine mesenchymal stem cells, human mesenchymal stem cells, and A549 human lung carcinoma cell line
Knockout Validated
Integrin beta1/CD29
is specifically detected in A549 human lung carcinoma parental cell line but is not detectable in
Integrin beta1/CD29 knockout A549 cell line.
Simple Western
10-20 µg/mL
Sample: HeLa human cervical epithelial carcinoma cell line, A431 human epithelial carcinoma cell line, Jurkat human acute T cell leukemia cell line, and U‑87 MG human glioblastoma/astrocytoma cell line
Sample: HeLa human cervical epithelial carcinoma cell line, A431 human epithelial carcinoma cell line, Jurkat human acute T cell leukemia cell line, and U‑87 MG human glioblastoma/astrocytoma cell line
Western Blot
1 µg/mL
Sample: MG‑63 human osteosarcoma cell line, HeLa human cervical epithelial carcinoma cell line, A431 human epithelial carcinoma cell line, Jurkat human acute T cell leukemia cell line, and U‑87 MG human glioblastoma/astrocytoma cell line
Sample: MG‑63 human osteosarcoma cell line, HeLa human cervical epithelial carcinoma cell line, A431 human epithelial carcinoma cell line, Jurkat human acute T cell leukemia cell line, and U‑87 MG human glioblastoma/astrocytoma cell line
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Integrin beta 1/CD29
Alternate Names
CD29, ITGB1
Gene Symbol
ITGB1
UniProt
Additional Integrin beta 1/CD29 Products
Product Documents for Human/Canine Integrin beta1/CD29 Antibody
Product Specific Notices for Human/Canine Integrin beta1/CD29 Antibody
For research use only
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