Detects human Integrin beta1 in Western blots. In Western blots, approximately 20% cross-reactivity with recombinant mouse (rm) Integrin beta1 is observed and less than 1% cross-reactivity recombinant human (rh) Integrin beta2, rhIntegrin beta3, rmIntegrin beta6, and rmIntegrin beta7 is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human Integrin beta1/CD29 Biotinylated Antibody

Detection of Integrin beta1/CD29 in Human PBMCs by Flow Cytometry.

Human peripheral blood mononuclear cells (PBMCs) were stained with Goat Anti-Human Integrin beta1/CD29 Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF1778, filled histogram) or isotype control antibody (Catalog # BAF108, open histogram), followed by Streptavidin-Phycoerythrin (Catalog # F0040).

Applications for Human Integrin beta1/CD29 Biotinylated Antibody

Application

Recommended Usage

Flow Cytometry

0.25 µg/10⁶ cells
Sample: Human peripheral blood mononuclear cells (PBMCs)

Western Blot

0.1 µg/mL
Sample: Recombinant Human Integrin beta1/CD29

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Integrin beta 1/CD29

Integrin beta1, also called CD29 and VLA-beta chain, associates with at least ten different integrin alpha subunits to form various VLA complexes. The beta1 subunit has a broad tissue distribution except erythrocytes.

Alternate Names

CD29, ITGB1

Entrez Gene IDs

3688 (Human); 16412 (Mouse); 102144610 (Cynomolgus Monkey)

Gene Symbol

ITGB1

UniProt

P05556

Additional Integrin beta 1/CD29 Products

Product Documents for Human Integrin beta1/CD29 Biotinylated Antibody

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Product Specific Notices for Human Integrin beta1/CD29 Biotinylated Antibody

For research use only

Citations
Reviews

Calculators
Flow Cytometry

Human Integrin beta1/CD29 Biotinylated Antibody

R&D Systems, part of Bio-Techne | Catalog # BAF1778

Key Product Details

Species Reactivity

Validated:

Cited:

Applications