Human B7-H3 Fluorescein-conjugated Antibody
R&D Systems, part of Bio-Techne | Catalog # FAB1027F
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human, Mouse
Applications
Validated:
Flow Cytometry
Cited:
Western Blot, Flow Cytometry, Co-Immunoprecipitation
Label
FITC (Excitation = 488 nm, Emission = 515-545 nm)
Antibody Source
Monoclonal Mouse IgG1 Clone # 185504
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant human B7-H3
Leu29-Pro245
Accession # NP_079516
Leu29-Pro245
Accession # NP_079516
Specificity
Detects human B7-H3 in direct ELISAs and Western blots. In Western blots, does not cross-react with recombinant human (rh) B7‑H1, rhB7‑H2, rhB7‑1, rhB7‑2, or recombinant mouse PD-L2.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for Human B7-H3 Fluorescein-conjugated Antibody
Detection of B7‑H3 in PC‑3 Human Cell Line by Flow Cytometry.
PC-3 human prostate cancer cell line was stained with Mouse Anti-Human B7-H3 Fluorescein-conjugated Monoclonal Antibody (Catalog # FAB1027F, filled histogram) or isotype control antibody (Catalog # IC002F, open histogram). View our protocol for Staining Membrane-associated Proteins.Applications for Human B7-H3 Fluorescein-conjugated Antibody
Application
Recommended Usage
Flow Cytometry
10 µL/106 cells
Sample: PC‑3 human prostate cancer cell line
Sample: PC‑3 human prostate cancer cell line
Spectra Viewer
Plan Your Experiments
Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.
Spectra Viewer
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
- 12 months from date of receipt, 2 to 8 °C as supplied.
Background: B7-H3
Long Name
B7 Homolog 3
Alternate Names
B7H3, CD276
Gene Symbol
CD276
UniProt
Additional B7-H3 Products
Product Documents for Human B7-H3 Fluorescein-conjugated Antibody
Product Specific Notices for Human B7-H3 Fluorescein-conjugated Antibody
For research use only
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