Human B7-1/CD80 Antibody

Name: Human B7-1/CD80 Antibody
Brand: R&D Systems
SKU: AF140
Price: 182 EUR

R&D Systems, Inc. a Bio-Techne Brand

Catalog # AF140

Citations (8)

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Species Reactivity:	Human
Immunogen:	S. frugiperda insect ovarian cell line Sf 21-derived recombinant human B7-1/CD80 Extracellular domain
Applications:	Immunohistochemistry, Western Blot
Label:	Unconjugated
Source:	Polyclonal Goat IgG
Specificity:	Detects human B7-1/CD80 in direct ELISAs and Western blots. In Western blots, approximately 5% cross-reactivity with recombinant human B7-2 and recombinant mouse B7-2 is observed.

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Applications for Human B7-1/CD80 Antibody

	Recommended Concentration	Sample
Western Blot	1 µg/mL	See below
Immunohistochemistry	5-15 µg/mL	See below

Please Note: Optimal dilutions should be determined by each laboratory for each application.

Scientific Data Examples for Human B7-1/CD80 Antibody

	Western Blot Detection of Human B7‑1/CD80 by Western Blot. Western blot shows lysates of Daudi human Burkitt's lymphoma cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human B7-1/CD80 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF140) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for B7-1/CD80 at approximately 60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
	Immunohistochemistry B7‑1/CD80 in Human Tonsil. B7-1/CD80 was detected in immersion fixed paraffin-embedded sections of human tonsil using Goat Anti-Human B7-1/CD80 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF140) at 3 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to lymphocytes in germinal center. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Formulation, Preparation and Storage

Purification	Antigen Affinity-purified
Reconstitution	Reconstitute at 0.2 mg/mL in sterile PBS. Reconstitution Buffer Available: Size / Price Qty Loading... RB01:
Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.

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Background: B7-1/CD80

B7-1 and B7-2, together with their receptors CD28 and CTLA-4, constitute one of the dominant co-stimulatory pathways that regulate T and B cell responses. Although both CTLA-4 and CD28 can bind to the same ligands, CTLA-4 binds to B7-1 and B7-2 with a 20-100 fold higher affinity than CD28 and is involved in the
down‑regulation of the immune response. B7-1 is expressed on activated B cells, activated T cells, and macrophages. B7-2 is constitutively expressed on interdigitating dendritic cells, Langerhans cells, peripheral blood dendritic cells, memory B cells, and germinal center B cells. Additionally, B7-2 is expressed at low levels on monocytes and can be up‑regulated through IFN-gamma. B7-1 and B7-2 are both members of the Immunoglobulin superfamily. Human B7-1 is a 288 amino acid (aa) protein containing a 34 aa signal peptide, a 208 aa extracellular domain, a 21 aa transmembrane domain, and a 25 aa cytoplasmic domain. Human B7-1 and B7-2 share 26% amino acid identity. Human and mouse B7-1 share 44% amino acid identity. However, it has been observed that both human and mouse B7-1 and B7-2 can bind to either human or mouse CD28 and CTLA-4, suggesting that there are conserved amino acids which form theB7-1/B7-2/CD28/CTLA-4 critical binding sites.

Item	Value
References	Azuma, M. et al. (1993) Nature 366:76. Freeman, G.J. et al. (1993) Science 262:909. Freeman, G. et al. (1991) J. Exp. Med. 174:625. Selvakumar, A. et al. (1993) Immunogenetics 38:292. Chen, C. et al. (1994) J. Immunol. 152:4929. Freeman, G.J. et al. (1993) J. Exp. Med. 178:2185.
Entrez Gene IDs	941 (Human); 12519 (Mouse); 25408 (Rat); 102140078 (Cynomolgus Monkey)
Alternate Names	Activation B7-1 antigen, B7, B7-1, B71, BB1, CD28LG1, CD28LGB7-1 antigen), CD80, CD80 antigen, CD80 molecule, CTLA-4 counter-receptor B7.1, T-lymphocyte activation antigen CD80, costimulatory factor CD80, costimulatory molecule variant IgV-CD80

Citations for Human B7-1/CD80 Antibody (8)

Showing 1 - 8 of 8 citations Showing All

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Citations are publications that use Bio-Techne products. Selected citations for Human B7-1/CD80 Antibody include:

Species: Human
Sample Types: Whole Tissue
Applications: IHC

P Chen et al. (2020), Usefulness of the cytokines expression of Th1/Th2/Th17 and urinary CD80 excretion in adult-onset minimal change disease PeerJ, 8(0):e9854.
PMID: 33194357
Species: Human
Sample Types: Whole Cells
Applications: ICC

T Hirano et al. (2018), Peripheral alveolar nitric oxide concentration reflects alveolar inflammation in autoimmune pulmonary alveolar proteinosis ERJ Open Res, 4(1).
PMID: 29577041
Species: Human
Sample Types: Whole Tissue
Applications: IHC-P

P Ai et al. (2017), Tumor microenvironment contributes to Epstein-Barr virus anti-nuclear antigen-1 antibody production in nasopharyngeal carcinoma Oncol Lett, 14(2):2458-2462.
PMID: 28781683
Species: Human
Sample Types: Whole Tissue
Applications: IHC-P

Mulder DJ et al. (2011), Antigen presentation and MHC class II expression by human esophageal epithelial cells: role in eosinophilic esophagitis. Am. J. Pathol., 178(2):744-53.
PMID: 21281807
Species: Human
Sample Types: Urine
Applications: Western Blot

Garin EH et al. (2010), Urinary CD80 is elevated in minimal change disease but not in focal segmental glomerulosclerosis. Kidney Int., 78(3):296-302.
PMID: 20485332
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry

Kuhne M et al. (2009), HLA-B27-restricted antigen presentation by human chondrocytes to CD8+ T cells: potential contribution to local immunopathologic processes in ankylosing spondylitis. Arthritis Rheum., 60(6):1635-46.
PMID: 19479861
Species: Human
Sample Types: Plasma
Applications: ELISA Development

Sakthivel P et al. (2007), The soluble forms of CD28, CD86 and CTLA-4 constitute possible immunological markers in patients with abdominal aortic aneurysm. J. Intern. Med., 261(4):399-407.
PMID: 17391115
Species: Human
Sample Types: Whole Tissue
Applications: IHC-Fr

Reiser J et al. (2004), Induction of B7-1 in podocytes is associated with nephrotic syndrome. J. Clin. Invest., 113(10):1390-7.
PMID: 15146236

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Human B7-1/CD80 Antibody

R&D Systems, Inc. a Bio-Techne Brand

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Applications for Human B7-1/CD80 Antibody

Scientific Data Examples for Human B7-1/CD80 Antibody

Western Blot

Immunohistochemistry

Formulation, Preparation and Storage

Reconstitution Calculator

Background: B7-1/CD80

Citations for Human B7-1/CD80 Antibody (8)

Product Documents for Human B7-1/CD80 Antibody

Product Datasheets

COA

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SDS

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