Human Adiponectin/Acrp30 Antibody

Name: Human Adiponectin/Acrp30 Antibody
Brand: rnd
SKU: AF1065

Catalog # AF1065

Citations (10)

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Key Product Details

Species Reactivity:	Human
Immunogen:	Mouse myeloma cell line NS0-derived recombinant human Adiponectin/Acrp30 Glu19-Asn244 Accession # Q15848
Applications:	Simple Western, Western Blot
Label:	Unconjugated
Source:	Polyclonal Goat IgG
Specificity:	Detects human Adiponectin/Acrp30 in direct ELISAs and Western blots. In direct ELISAs, less than 30% cross‑reactivity with recombinant mouse Adiponectin is observed and less than 15% cross-reactivity with recombinant rat Adiponectin is observed.

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Applications

	Recommended Concentration	Sample
Western Blot	1 µg/mL	See below
Simple Western	10 µg/mL	See below

Please Note: Optimal dilutions should be determined by each laboratory for each application.

Human Adiponectin/Acrp30 Antibody Scientific Data Examples

	Western Blot Detection of Human Adiponectin/Acrp30 by Western Blot. Western blot shows lysates of human placenta tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human Adiponectin/Acrp30 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1065) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for Adiponectin/Acrp30 at approximately 30-32 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
	Simple Western Detection of Human Adiponectin/Acrp30 by Simple Western^TM. Simple Western lane view shows lysates of human placenta tissue, loaded at 0.2 mg/mL. A specific band was detected for Adiponectin/Acrp30 at approximately 41 kDa (as indicated) using 10 µg/mL of Goat Anti-Human Adiponectin/Acrp30 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1065) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Formulation, Preparation and Storage

Purification	Antigen Affinity-purified
Reconstitution	Reconstitute at 0.2 mg/mL in sterile PBS. Reconstitution Buffer Available: Size / Price Qty Loading... RB01:
Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.

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Background: Adiponectin/Acrp30

Adiponectin, also known as Acrp30, is an adipocyte-derived protein with wide ranging paracrine and endocrine effects on metabolism and inflammation. It is induced during adipocyte differentiation, and its secretion is stimulated by insulin. It promotes adipocyte differentiation, fatty acid catabolism, and insulin sensitivity and is negatively correlated with obesity, type 2 diabetes, and atherogenesis. In this context, Adiponectin is an anti-inflammatory agent, but it exerts pro-inflammatory effects in nonmetabolic disorders such as rheumatoid arthritis and inflammatory bowel disease (1-3). Adiponectin interacts with the receptors AdipoR1 and AdipoR2, calreticulin, and Cadherin-13/T-Cadherin, as well as with several growth factors (4-7). Mature human Adiponectin consists of a 60 amino acid (aa) N-terminal collagenous region and a 137 aa C-terminal C1q-like globular domain which can be cleaved by a leukocyte-derived elastase (8-9). Mature human Adiponectin shares 83% and 85% amino acid (aa) sequence identity with mouse and rat Adiponectin, respectively. Adiponectin associates into trimers that may assemble into medium molecular weight (MMW) hexamers and then into > 300 kDa high molecular weight (HMW) oligomers (10-12). The glycosylation of four hydroxylated lysine residues in the collagenous domain is required for the intracellular formation of HMW complexes (13). The various multimeric forms of Adiponectin exhibit distinct tissue specific and gender specific profiles and activities (12, 14).

Item	Value
References	Lara-Castro, C. et al. (2007) Curr. Opin. Lipidol. 18:263. Tilg, H. and A.R. Moschen (2006) Nat. Rev. Immunol. 6:772. Fantuzzi, G. (2008) J. Allergy Clin. Immunol. 121:326. Yamauchi, T. et al. (2007) Nat. Med. 13:332. Takemura, Y. et al. (2007) J. Clin. Invest. 117:375. Hug, C. et al. (2004) Proc. Natl. Acad. Sci. 101:10308. Wang, Y. et al. (2005) J. Biol. Chem. 280:18341. Maeda, K. et al. (1996) Biochem. Biophys. Res. Commun. 221:286. Waki, H. et al. (2005) Endocrinology 146:790. Waki, H. et al. (2003) J. Biol. Chem. 278:40352. Tsao, T.S. et al. (2003) J. Biol. Chem. 278:50810. Wang, Y. et al. (2008) Biochem. J. 409:623. Wang, H. et al. (2006) J. Biol. Chem. 281:16391. Pajvani, U.B. et al. (2003) J. Biol. Chem. 278:9073.
Entrez Gene IDs	9370 (Human); 11450 (Mouse); 246253 (Rat)
Alternate Names	ACDC, ACRP30ADPN, ADIPQTL1, APM1APM-1, Acrp30, AdipoQ, Adiponectin, ApM1, C1q and collagen domain-containing protein, GBP28, GBP28apM1, Gelatin-binding protein, adipocyte, C1Q and collagen domain containing, adiponectin, C1Q and collagen domain containing, apM-1

Citations (10)

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Citations are publications that use Bio-Techne products. Selected citations for Human Adiponectin/Acrp30 Antibody include:

Species: Mouse
Sample Types: Whole Tissue
Applications: IHC

B Hu et al. (2020), Sensory nerves regulate mesenchymal stromal cell lineage commitment by tuning sympathetic tones J. Clin. Invest., 0(0):.
PMID: 32191640
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot

FJ Ruiz-Ojeda et al. (2019), Effects of X-chromosome Tenomodulin Genetic Variants on Obesity in a Children's Cohort and Implications of the Gene in Adipocyte Metabolism Sci Rep, 9(1):3979.
PMID: 30850679
Species: Human
Sample Types: Plasma
Applications: Western Blot

K Tang et al. (2017), Sustained High Levels of Both Total and High Molecular Weight Adiponectin in Plasma during the Convalescent Phase of Haemorrhagic Fever with Renal Syndrome Are Associated with Disease Severity J Immunol Res, 2017(0):6468097.
PMID: 28424792
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot

FJ Ruiz-Ojeda et al. (2016), Impact of 3-Amino-1,2,4-Triazole (3-AT)-Derived Increase in Hydrogen Peroxide Levels on Inflammation and Metabolism in Human Differentiated Adipocytes PLoS ONE, 11(3):e0152550.
PMID: 27023799
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot

Beier E et al. (2015), Heavy Metal Ion Regulation of Gene Expression: MECHANISMS BY WHICH LEAD INHIBITS OSTEOBLASTIC BONE-FORMING ACTIVITY THROUGH MODULATION OF THE Wnt/beta-CATENIN SIGNALING PATHWAY. J Biol Chem, 290(29):18216-26.
PMID: 25975268
Species: Human
Sample Types: Urine
Applications: Western Blot

von Eynatten M et al. (2009), Urinary adiponectin excretion: a novel marker for vascular damage in type 2 diabetes. Diabetes, 58(9):2093-9.
PMID: 19509019
Species: Human
Sample Types: Whole Tissue
Applications: IHC-P

Miller M et al. (2009), Adiponectin and functional adiponectin receptor 1 are expressed by airway epithelial cells in chronic obstructive pulmonary disease. J. Immunol., 182(1):684-91.
PMID: 19109202
Species: Human
Sample Types: Serum
Applications: Western Blot

Retnakaran R et al. (2007), Decreased high-molecular-weight adiponectin in gestational diabetes: implications for the pathophysiology of Type 2 diabetes. Diabet. Med., 24(3):245-52.
PMID: 17305786
Species: Human
Sample Types: CSF
Applications: Native SDS-PAGE

Ebinuma H et al. (2007), Improved ELISA for selective measurement of adiponectin multimers and identification of adiponectin in human cerebrospinal fluid. Clin. Chem., 53(8):1541-4.
PMID: 17599956
Species: Human
Sample Types: Whole Cells
Applications: Neutralization

Iversen PO et al. (2005), Tumor necrosis factor alpha and adiponectin in bone marrow interstitial fluid from patients with acute myeloid leukemia inhibit normal hematopoiesis. Clin. Cancer Res., 11(19):6793-9.
PMID: 16203766

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Human Adiponectin/Acrp30 Antibody

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Key Product Details

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Applications

Human Adiponectin/Acrp30 Antibody Scientific Data Examples

Western Blot

Simple Western

Formulation, Preparation and Storage

Reconstitution Calculator

Background: Adiponectin/Acrp30

Citations (10)

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