Human Adiponectin/Acrp30 Biotinylated Antibody

Name: Human Adiponectin/Acrp30 Biotinylated Antibody
Brand: R&D Systems
SKU: BAF1065

R&D Systems, Inc. a Bio-Techne Brand

Catalog # BAF1065

Citations (1)

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Species Reactivity:	Human
Immunogen:	Mouse myeloma cell line NS0-derived recombinant human Adiponectin/Acrp30 (R&D Systems, Catalog # 1065-AP) Glu19-Asn244 Accession # Q15848
Applications:	Western Blot
Label:	Biotin
Source:	Polyclonal Goat IgG
Specificity:	Detects human Adiponectin/Acrp30 in Western blots. In this format, approximately 5% cross-reactivity with recombinant mouse Adiponectin is observed.

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Applications for Human Adiponectin/Acrp30 Biotinylated Antibody

	Recommended Concentration	Sample
Western Blot	0.1 µg/mL	Recombinant Human Adiponectin/Acrp30 (Catalog # 1065-AP)

Please Note: Optimal dilutions should be determined by each laboratory for each application.

Formulation, Preparation and Storage

Purification	Antigen Affinity-purified
Reconstitution	Reconstitute at 0.2 mg/mL in sterile PBS. Reconstitution Buffer Available: Size / Price Qty Loading... RB01:
Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.

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Background: Adiponectin/Acrp30

Adiponectin, also known as Acrp30, is an adipocyte-derived protein with wide ranging paracrine and endocrine effects on metabolism and inflammation. It is induced during adipocyte differentiation, and its secretion is stimulated by insulin. It promotes adipocyte differentiation, fatty acid catabolism, and insulin sensitivity and is negatively correlated with obesity, type 2 diabetes, and atherogenesis. In this context, Adiponectin is an anti-inflammatory agent, but it exerts pro-inflammatory effects in nonmetabolic disorders such as rheumatoid arthritis and inflammatory bowel disease (1-3). Adiponectin interacts with the receptors AdipoR1 and AdipoR2, calreticulin, and Cadherin-13/T-Cadherin, as well as with several growth factors (4-7). Mature human Adiponectin consists of a 60 amino acid (aa) N-terminal collagenous region and a 137 aa C-terminal C1q-like globular domain which can be cleaved by a leukocyte-derived elastase (8-9). Mature human Adiponectin shares 83% and 85% amino acid (aa) sequence identity with mouse and rat Adiponectin, respectively. Adiponectin associates into trimers that may assemble into medium molecular weight (MMW) hexamers and then into > 300 kDa high molecular weight (HMW) oligomers (10-12). The glycosylation of four hydroxylated lysine residues in the collagenous domain is required for the intracellular formation of HMW complexes (13). The various multimeric forms of Adiponectin exhibit distinct tissue specific and gender specific profiles and activities (12, 14).

Item	Value
References	Lara-Castro, C. et al. (2007) Curr. Opin. Lipidol. 18:263. Tilg, H. and A.R. Moschen (2006) Nat. Rev. Immunol. 6:772. Fantuzzi, G. (2008) J. Allergy Clin. Immunol. 121:326. Yamauchi, T. et al. (2007) Nat. Med. 13:332. Takemura, Y. et al. (2007) J. Clin. Invest. 117:375. Hug, C. et al. (2004) Proc. Natl. Acad. Sci. 101:10308. Wang, Y. et al. (2005) J. Biol. Chem. 280:18341. Maeda, K. et al. (1996) Biochem. Biophys. Res. Commun. 221:286. Waki, H. et al. (2005) Endocrinology 146:790. Waki, H. et al. (2003) J. Biol. Chem. 278:40352. Tsao, T.S. et al. (2003) J. Biol. Chem. 278:50810. Wang, Y. et al. (2008) Biochem. J. 409:623. Wang, H. et al. (2006) J. Biol. Chem. 281:16391. Pajvani, U.B. et al. (2003) J. Biol. Chem. 278:9073.
Entrez Gene IDs	9370 (Human); 11450 (Mouse); 246253 (Rat)
Alternate Names	ACDC, ACRP30ADPN, ADIPQTL1, APM1APM-1, Acrp30, AdipoQ, Adiponectin, ApM1, C1q and collagen domain-containing protein, GBP28, GBP28apM1, Gelatin-binding protein, adipocyte, C1Q and collagen domain containing, adiponectin, C1Q and collagen domain containing, apM-1

Citations for Human Adiponectin/Acrp30 Biotinylated Antibody (1)

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Citations are publications that use Bio-Techne products. Selected citations for Human Adiponectin/Acrp30 Biotinylated Antibody include:

Species: Human
Sample Types: Whole Tissue
Applications: IHC-P

von Eynatten M et al. (2009), Urinary adiponectin excretion: a novel marker for vascular damage in type 2 diabetes. Diabetes, 58(9):2093-9.
PMID: 19509019

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Human Adiponectin/Acrp30 Biotinylated Antibody

R&D Systems, Inc. a Bio-Techne Brand

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