Human 4-1BB Ligand/TNFSF9 Biotinylated Antibody

Name: Human 4-1BB Ligand/TNFSF9 Biotinylated Antibody
Brand: R&D Systems
SKU: BAF2295
Rating: 5 (1 reviews)

R&D Systems, Inc. a Bio-Techne Brand

Catalog # BAF2295

Citations (2)

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Species Reactivity:	Human
Immunogen:	E. coli-derived recombinant human 4-1BB Ligand Arg71-Glu254 Accession # P41273
Applications:	Flow Cytometry, Western Blot
Label:	Biotin
Source:	Polyclonal Goat IgG
Specificity:	Detects human 4‑1BB Ligand/TNFSF9 in Western blots. In Western blots, less than 1% cross‑reactivity with recombinant mouse (rm) 4‑1BB Ligand, recombinant human (rh) APRIL, rhBAFF, rhEDA-A2, rhFas Ligand, rhGITR Ligand, rhLIGHT, rhOX40 Ligand, rhTNF-alpha, rhTRAIL, and rhTWEAK is observed.

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Applications for Human 4-1BB Ligand/TNFSF9 Biotinylated Antibody

	Recommended Concentration	Sample
Western Blot	0.1 µg/mL	Recombinant Human 4‑1BB Ligand/TNFSF9 (Catalog # 2295-4L)
Flow Cytometry	0.25 µg/10⁶ cells	Daudi human Burkitt's lymphoma cell line

Please Note: Optimal dilutions should be determined by each laboratory for each application.

Scientific Data Examples for Human 4-1BB Ligand/TNFSF9 Biotinylated Antibody

	Flow Cytometry Detection of 4‑1BB Ligand/TNFSF9 in Human Daudi cell line by Flow Cytometry. Human Daudi Burkitt lymphoma cell line was stained with Goat Anti-Human 4-1BB Ligand/TNFSF9 Biotinylated Polyclonal Antibody (Catalog # BAF2295, filled histogram) or control antibody (BAF108, open histogram), followed by PE-conjugated Streptavidin (F0040). Staining was performed usinig our Staining Membrane-associated Proteins protocol.

Formulation, Preparation and Storage

Purification	Antigen Affinity-purified
Reconstitution	Reconstitute at 0.2 mg/mL in sterile PBS. Reconstitution Buffer Available: Size / Price Qty Loading... RB01:
Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.

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Background: 4-1BB Ligand/TNFSF9

4-1BB ligand (4-1BBL; also CD137L) is a 32 kDa type II transmembrane protein that belongs to the TNF superfamily (TNFSF) molecules (1‑4). The human 4-1BBL cDNA encodes a 254 amino acid (aa) protein that contains a 25 aa N-terminal cytoplasmic domain, a 23 aa transmembrane segment, and a 206 aa C-terminal extracellular region (5). The extracellular domain (ECD) of 4-1BBL has a jelly-roll, beta -sandwich tertiary structure that is similar to other TNFSF members. There is only one cysteine in the human ECD, and no potential N-linked glycosylation sites. The potential exists, however, for O-linked glycosylation. The human 4-1BBL ECD shares 32% and 35% aa identity with mouse and rat ECD, respectively. In the cytoplasmic domain, human 4-1BBL is 55 aa shorter than the equivalent region in rodents. 4-1BBL is expressed by activated B cells, macrophages, dendritic cells, activated T cells, neurons and astrocytes (2, 3, 6). A 26 kDa soluble form of 4-1BBL is known to occur in humans. Although it is presumably generated by MMP activity, its amino acid size is currently unreported (4). The soluble form is bioactive. Human 4-1BBL signals through both CD137/4-1BB and itself. Its cytoplasmic tail participates in reverse signaling that induces apoptosis in T cells and cytokine secretion (IL-6; TNF-alpha ) by monocytes (7, 8). 4-1BBL binding to CD137/4-1BB produces a number of effects. It seems to play a key role in the T cell recall response. It maintains T cell numbers at the end of a primary response, and induces CD4⁺ and CD8⁺ T cells to proliferate and secrete cytokines such as IL-2 and IFN-gamma in CD4⁺ cells, and IFN-gamma in CD8⁺ cells (9, 10).

Item	Value
References	Kwon, B. et al. (2003) Exp. Mol. Med. 35:8. Salih, H.R. et al. (2002) Int. J. Clin. Pharmacol. Ther. 40:348. Vinay, D.S. and B.S. Kwon (1998) Semin. Immunol. 1:481. Salih, H.R. et al. (2001) J. Immunol. 167:4059. Alderson, M.R. et al. (1994) Eur. J. Immunol. 24:2219. Reali, C. et al. (2003) J. Neurosci. Res. 74:67. Michel, J. et al. (1999) Immunology 98:42. Langstein, J. et al. (1998) J. Immunol. 160:2488. Wen, T. et al. (2002) J. Immunol. 168:4897. Dawicki, W. and T.H. Watts (2004) Eur. J. Immunol. 34:743.
Entrez Gene IDs	8744 (Human); 21950 (Mouse); 353218 (Rat); 102129676 (Cynomolgus Monkey)
Alternate Names	4-1BB Ligand, 4-1BB-L, 4-1BBL, 41BB Ligand, CD137L, TNFSF9, homolog of mouse 4-1BB-L, receptor 4-1BB ligand, tumor necrosis factor (ligand) superfamily, member 9, tumor necrosis factor ligand superfamily member 9

Citations for Human 4-1BB Ligand/TNFSF9 Biotinylated Antibody (2)

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Citations are publications that use Bio-Techne products. Selected citations for Human 4-1BB Ligand/TNFSF9 Biotinylated Antibody include:

Species: Human
Sample Types: Whole Cells
Applications: IHC

C Geuijen et al. (2021), A human CD137ï¿½PD-L1 bispecific antibody promotes anti-tumor immunity via context-dependent T cell costimulation and checkpoint blockade Nature Communications, 12(1):4445.
PMID: 34290245
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot

RN Gilbreth et al. (2018), Crystal structure of the human 4-1BB/4-1BBL complex J. Biol. Chem., 0(0).
PMID: 29720399

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Customer Reviews for Human 4-1BB Ligand/TNFSF9 Biotinylated Antibody (1)

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Human 4-1BB Ligand/TNFSF9 Biotinylated Antibody

Name: Anonymous

Application: ELISA

Sample Tested: HT1080 human fibrosarcoma cell line

Species: Human

Verified Customer | Posted 03/09/2023

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Human 4-1BB Ligand/TNFSF9 Biotinylated Antibody

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Applications for Human 4-1BB Ligand/TNFSF9 Biotinylated Antibody

Scientific Data Examples for Human 4-1BB Ligand/TNFSF9 Biotinylated Antibody

Flow Cytometry

Formulation, Preparation and Storage

Reconstitution Calculator

Background: 4-1BB Ligand/TNFSF9

Citations for Human 4-1BB Ligand/TNFSF9 Biotinylated Antibody (2)

Customer Reviews for Human 4-1BB Ligand/TNFSF9 Biotinylated Antibody (1)

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Human 4-1BB Ligand/TNFSF9 Biotinylated Antibody

Product Documents for Human 4-1BB Ligand/TNFSF9 Biotinylated Antibody

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