Human 4-1BB Ligand/TNFSF9 Antibody

Name: Human 4-1BB Ligand/TNFSF9 Antibody
Brand: R&D Systems
SKU: AF2295
Rating: 5 (2 reviews)

R&D Systems, Inc. a Bio-Techne Brand

Catalog # AF2295

Citations (3)

(2)

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Species Reactivity:	Human
Immunogen:	E. coli-derived recombinant human 4‑1BB Ligand/TNFSF9 Arg71-Glu254 Accession # P41273
Applications:	CyTOF-ready, Flow Cytometry, Immunohistochemistry, Western Blot
Label:	Unconjugated
Source:	Polyclonal Goat IgG
Specificity:	Detects human 4‑1BB Ligand/TNFSF9 in direct ELISAs and Western blots.

View Full Technical Details for Human 4-1BB Ligand/TNFSF9 Antibody

Applications for Human 4-1BB Ligand/TNFSF9 Antibody

	Recommended Concentration	Sample
Western Blot	1 µg/mL	See below
Flow Cytometry	2.5 µg/10⁶ cells	Daudi human Burkitt's lymphoma cell line
Immunohistochemistry	1-15 µg/mL	See below
CyTOF-ready	Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Please Note: Optimal dilutions should be determined by each laboratory for each application.

Scientific Data Examples for Human 4-1BB Ligand/TNFSF9 Antibody

	Western Blot Detection of Human 4‑1BB Ligand/TNFSF9 by Western Blot. Western blot shows lysates of human placenta tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human 4-1BB Ligand/TNFSF9 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2295) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (HAF017). A specific band was detected for 4-1BB Ligand/TNFSF9 at approximately 26 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
	Immunohistochemistry 4‑1BB Ligand/TNFSF9 in Human Placenta. 4-1BB Ligand/TNFSF9 was detected in immersion fixed paraffin-embedded sections of human placenta using Goat Anti-Human 4-1BB Ligand/TNFSF9 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2295) at 1 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (VC004). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to syncytiotrophoblasts. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Formulation, Preparation and Storage

Purification	Antigen Affinity-purified
Reconstitution	Reconstitute at 0.2 mg/mL in sterile PBS. Reconstitution Buffer Available: Size / Price Qty Loading... RB01:
Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.

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Background: 4-1BB Ligand/TNFSF9

4-1BB ligand (4-1BBL; also CD137L) is a 32 kDa type II transmembrane protein that belongs to the TNF superfamily (TNFSF) molecules (1‑4). The human 4-1BBL cDNA encodes a 254 amino acid (aa) protein that contains a 25 aa N-terminal cytoplasmic domain, a 23 aa transmembrane segment, and a 206 aa C-terminal extracellular region (5). The extracellular domain (ECD) of 4-1BBL has a jelly-roll, beta -sandwich tertiary structure that is similar to other TNFSF members. There is only one cysteine in the human ECD, and no potential N-linked glycosylation sites. The potential exists, however, for O-linked glycosylation. The human 4-1BBL ECD shares 32% and 35% aa identity with mouse and rat ECD, respectively. In the cytoplasmic domain, human 4-1BBL is 55 aa shorter than the equivalent region in rodents. 4-1BBL is expressed by activated B cells, macrophages, dendritic cells, activated T cells, neurons and astrocytes (2, 3, 6). A 26 kDa soluble form of 4-1BBL is known to occur in humans. Although it is presumably generated by MMP activity, its amino acid size is currently unreported (4). The soluble form is bioactive. Human 4-1BBL signals through both CD137/4-1BB and itself. Its cytoplasmic tail participates in reverse signaling that induces apoptosis in T cells and cytokine secretion (IL-6; TNF-alpha ) by monocytes (7, 8). 4-1BBL binding to CD137/4-1BB produces a number of effects. It seems to play a key role in the T cell recall response. It maintains T cell numbers at the end of a primary response, and induces CD4⁺ and CD8⁺ T cells to proliferate and secrete cytokines such as IL-2 and IFN-gamma in CD4⁺ cells, and IFN-gamma in CD8⁺ cells (9, 10).

Item	Value
References	Kwon, B. et al. (2003) Exp. Mol. Med. 35:8. Salih, H.R. et al. (2002) Int. J. Clin. Pharmacol. Ther. 40:348. Vinay, D.S. and B.S. Kwon (1998) Semin. Immunol. 1:481. Salih, H.R. et al. (2001) J. Immunol. 167:4059. Alderson, M.R. et al. (1994) Eur. J. Immunol. 24:2219. Reali, C. et al. (2003) J. Neurosci. Res. 74:67. Michel, J. et al. (1999) Immunology 98:42. Langstein, J. et al. (1998) J. Immunol. 160:2488. Wen, T. et al. (2002) J. Immunol. 168:4897. Dawicki, W. and T.H. Watts (2004) Eur. J. Immunol. 34:743.
Entrez Gene IDs	8744 (Human); 21950 (Mouse); 353218 (Rat); 102129676 (Cynomolgus Monkey)
Alternate Names	4-1BB Ligand, 4-1BB-L, 4-1BBL, 41BB Ligand, CD137L, TNFSF9, homolog of mouse 4-1BB-L, receptor 4-1BB ligand, tumor necrosis factor (ligand) superfamily, member 9, tumor necrosis factor ligand superfamily member 9

Citations for Human 4-1BB Ligand/TNFSF9 Antibody (3)

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Citations are publications that use Bio-Techne products. Selected citations for Human 4-1BB Ligand/TNFSF9 Antibody include:

Species: Human
Sample Types: Cell Lysates
Applications: Western Blot

J Joung et al. (2022), CRISPR activation screen identifies BCL-2 proteins and B3GNT2 as drivers of cancer resistance to T cell-mediated cytotoxicity Nature Communications, 13(1):1606.
PMID: 35338135
Species: Human
Sample Types: Whole Tissue
Applications: IHC-P

C Cochain et al. (2018), Single-Cell RNA-Seq Reveals the Transcriptional Landscape and Heterogeneity of Aortic Macrophages in Murine Atherosclerosis Circ. Res., 0(0).
PMID: 29545365
Species: Human
Sample Types: Whole Tissue
Applications: IHC-P

Ek S et al. (2006), Mantle cell lymphomas acquire increased expression of CCL4, CCL5 and 4-1BB-L implicated in cell survival. Int. J. Cancer, 118(8):2092-7.
PMID: 16287062

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Customer Reviews for Human 4-1BB Ligand/TNFSF9 Antibody (2)

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Human 4-1BB Ligand/TNFSF9 Antibody

Name: Anonymous

Application: ELISA

Sample Tested: Baby hamster kidney

Species: Human

Verified Customer | Posted 03/22/2023

Functions well at 2ug/mL.
Human 4-1BB Ligand/TNFSF9 Antibody

Name: Anonymous

Application: ELISA

Sample Tested: HT1080 human fibrosarcoma cell line

Species: Human

Verified Customer | Posted 03/09/2023

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Product Documents for Human 4-1BB Ligand/TNFSF9 Antibody

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Human 4-1BB Ligand/TNFSF9 Antibody

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Applications for Human 4-1BB Ligand/TNFSF9 Antibody

Scientific Data Examples for Human 4-1BB Ligand/TNFSF9 Antibody

Western Blot

Immunohistochemistry

Formulation, Preparation and Storage

Reconstitution Calculator

Background: 4-1BB Ligand/TNFSF9

Citations for Human 4-1BB Ligand/TNFSF9 Antibody (3)

Customer Reviews for Human 4-1BB Ligand/TNFSF9 Antibody (2)

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Human 4-1BB Ligand/TNFSF9 Antibody

Human 4-1BB Ligand/TNFSF9 Antibody

Product Documents for Human 4-1BB Ligand/TNFSF9 Antibody

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