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Human 17 beta-HSD14/HSD17B14 Antibody

Bio-Techne includes R&D Systems | Catalog # MAB7515

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MAB7515
MAB7515-SP

Key Product Details

Species Reactivity

Validated:

Human

Applications

Immunohistochemistry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 736043

Product Summary for Human 17 beta-HSD14/HSD17B14 Antibody

Immunogen

E. coli-derived recombinant human 17 beta‑HSD14/HSD17B14
Ala2-Ser270
Accession # Q9BPX1

Specificity

Detects human 17 beta‑HSD14/HSD17B14 in direct ELISAs.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for Human 17 beta-HSD14/HSD17B14 Antibody

17 beta-HSD14/HSD17B14 antibody in Human Brain by Immunohistochemistry (IHC-P).

17 beta‑HSD14/HSD17B14 in Human Brain.

17 beta-HSD14/HSD17B14 was detected in immersion fixed paraffin-embedded sections of human brain (medulla) using Mouse Anti-Human 17 beta-HSD14/HSD17B14 Monoclonal Antibody (Catalog # MAB7515) at 15 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to neuronal cytoplasm. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Applications for Human 17 beta-HSD14/HSD17B14 Antibody

Application
Recommended Usage

Immunohistochemistry

8-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human brain (medulla)
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 2 reviews rated 5 using MAB7515 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.

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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: 17 beta-HSD14/HSD17B14

HSD-17b14 (hydroxysteroid (17-beta) dehydrogenase type14), also called 17bHSD14 or DHRS10, is a cytoplasmic 17beta-hydroxysteroid dehydrogenase that is a member of the SDR (short-chain dehydrogenase/reductase) family. It is a 270 amino acid, approximately 30 kDa protein with mRNA highly expressed in brain, placenta, liver and kidney. It converts estradiol to estrone, thus inactivating it, and is thought to regulate activity of estrogens and possibly dihydroepiandrosterone (DHEA) in the placenta and central nervous system. Human HSD-17b14 shares 80.5% amino acid sequence identity with mouse and rat HSD-17b14.

Long Name

17 beta Hydroxysteroid Dehydrogenase 14

Alternate Names

17 betaHSD14, DHRS10, HSD17B14, retSDR3, SDR3, SDR47C1

Entrez Gene IDs

51171 (Human); 66065 (Mouse); 691018 (Rat)

Gene Symbol

HSD17B14

UniProt

Additional 17 beta-HSD14/HSD17B14 Products

Product Documents for Human 17 beta-HSD14/HSD17B14 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human 17 beta-HSD14/HSD17B14 Antibody

For research use only

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