DUX4 Antibody (P4H2) [PE]

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-49552PE

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence

Label

PE (Excitation = 488 nm, Emission = 575 nm)

Antibody Source

Monoclonal Mouse IgG1 Clone # P4H2

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all DUX4 Antibodies »

Product Specifications

Immunogen

Synthetic peptide corresponding to the C-terminus of human DUX4.

Localization

Nucleus. Note: Actively transported through the nuclear pore complex (NPC)

Specificity

This antibody is specific for DUX4 and does not recognize DUX4c.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

52 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Applications for DUX4 Antibody (P4H2) [PE]

Application
Recommended Usage

ELISA

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Frozen

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Paraffin

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
This DUX4 antibody is useful for Immunocytochemistry/Immunofluorescence, Immunohistochemistry frozen sections, Immunohistochemistry paraffin embedded sections and Western Blot. In Western blot, a band can be seen at ~52 kDa. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark.

Background: DUX4

The DUX4 gene is located within a D4Z4 repeat array in the subtelomeric region of chromosome 4q. The D4Z4 repeat is polymorphic in length; a similar D4Z4 repeat array has been identified on chromosome 10. DUX4 (also known as Double homeobox 4) is the leading candidate causative gene for facioscapulohumeral dystrophy (FSHD), a degenerative skeletal muscle disease and one of the most common muscular dystrophies. FSHD is caused by the deletion of a subset of D4Z4 macrosatellite repeats on chromosome 4. Each repeat contains a retrogene encoding the double-homeobox factor DUX4. DUX4 expression is epigenetically suppressed in differentiated tissues and the residual DUX4 transcripts are spliced to remove the carboxyterminal domain that has been associated with cell toxicity. In FSHD individuals, the expression of the full-length DUX4 transcript is not completely suppressed in skeletal muscle, and possibly other differentiated tissues, and results in a small percentage of cells expressing relatively abundant amounts of the full-length DUX4 mRNA and protein. [Snider et al. (2010) PLoS Genetics.6(10): e1001181]

Long Name

Double Homeobox 4

Alternate Names

DUX10, DUX4L1

Gene Symbol

DUX4

Additional DUX4 Products

Product Documents for DUX4 Antibody (P4H2) [PE]

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for DUX4 Antibody (P4H2) [PE]

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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