B7-1/CD80 Antibody (62N3G8) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-80588

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Human, Mouse

Applications

Multiplex Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2b Kappa Clone # 62N3G8

Format

Azide and BSA Free

Concentration

1.0 mg/ml

View all B7-1/CD80 Antibodies »

Product Specifications

Immunogen

Amino acids 102-223 of human CD80 were used as the immunogen for the antibody.

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 27440778).

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2b Kappa

Description

Novus Biologicals Mouse B7-1/CD80 Antibody (62N3G8) - Azide and BSA Free (NBP2-25255) is a monoclonal antibody validated for use in IHC, WB, Flow and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for B7-1/CD80 Antibody (62N3G8) - Azide and BSA Free

B7-1/CD80 Antibody (62N3G8) - Azide and BSA Free B7-1/CD80 Antibody (62N3G8) - BSA Free

Detection of B7-1/CD80 in Human Hodgkin's Lymphoma via seqIF™ staining on COMET™

B7-1/CD80 was detected in immersion fixed paraffin-embedded sections of human Hodgkin's Lymphoma using Mouse Anti-Human B7-1/CD80, Monoclonal Antibody at 20ug/mL at 37°Celsius for 4 minutes. Before incubation with the primary antibody, tissue underwent an all-in-one dewaxing and antigen retrieval preprocessing using PreTreatment Module (PT Module) and Dewax and HIER Buffer H (pH 9; Epredia Catalog # TA-999-DHBH). Tissue was stained using the Alexa Fluor™ 647 Goat anti-Mouse IgG Secondary Antibody at 1:200 at 37 ° Celsius for 2 minutes. (Yellow; Lunaphore Catalog # DR647MS) and counterstained with DAPI (blue; Lunaphore Catalog # DR100). Specific staining was localized to the membrane. Protocol available in COMET™ Panel Builder.
Western Blot: B7-1/CD80 Antibody (62N3G8)Azide and BSA Free [NBP2-80588]

Western Blot: B7-1/CD80 Antibody (62N3G8)Azide and BSA Free [NBP2-80588]

Western Blot: B7-1/CD80 Antibody (62N3G8) - Azide and BSA Free [NBP2-80588] - Analysis of CD80 in A) a partial recombinant protein and B) Daudi lysate using this antibody at 0.1 and 1 g/ml, respectively. Goat anti-mouse Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test. Image from the standard format of this antibody.
Immunocytochemistry: B7-1/CD80 Antibody (62N3G8) - Azide and BSA Free [NBP2-80588]

Immunocytochemistry: B7-1/CD80 Antibody (62N3G8) - Azide and BSA Free [NBP2-80588]

Immunocytochemistry: B7-1/CD80 Antibody (62N3G8) - Azide and BSA Free [NBP2-80588] - Human thymus stained with CD80 antibody at 5 ug/ml using peroxidase-conjugate and DAB chromogen. Note membrane and cytoplasmic staining. Image from the standard format of this antibody.
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Applications for B7-1/CD80 Antibody (62N3G8) - Azide and BSA Free

Application
Recommended Usage

Flow Cytometry

1:10-1:1000. Use reported by customer review

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Paraffin

1:10-1:500

Western Blot

reported by customer review
Application Notes
Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM sodium citrate buffer, pH 6.0 for 10-20 min followed by cooling at RT for 20 min.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: B7-1/CD80

The CD80 antigen (B7-1, BB1) recognizes a 60-kD transmembrane glycoprotein that is a member of the immunoglobulin family. This molecule shares with CD86 the capability to be the ligand for two structurally similar molecules expressed on T lymphocytes, CD28 and CD152 (CTLA-4). CD80 antigen is expressed on in vitro activated B lymphocytes after 24h stimulation and at maximal level 48-72h post activation. It is not expressed on the majority of resting B cells from peripheral blood but identifies a subpopulation of B cells that has been previously activated. The antigen is also expressed by HTLV-1 transformed T cells activated monocytes, and constitutively on dendritic cells. CD80 binding provides costimulatory signals for T cell activation.

Alternate Names

B71, CD80

Gene Symbol

CD80

Additional B7-1/CD80 Products

Product Documents for B7-1/CD80 Antibody (62N3G8) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for B7-1/CD80 Antibody (62N3G8) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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