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ABCA7 Antibody (7A1-144), Novus Biologicals

Bio-Techne includes Novus Biologicals | Catalog # NB400-163

Key Product Details

Species Reactivity

Validated:

Mouse

Applications

Flow Cytometry, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG2a Kappa Clone # 7A1-144

Concentration

1.0 mg/ml

Product Summary for ABCA7 Antibody (7A1-144)

Immunogen

Mouse ABCA7 stably-transfected HeLa cells. [UniProt# Q91V24]

Localization

Cell Membrane and Cytoplasmic

Clonality

Monoclonal

Host

Rat

Isotype

IgG2a Kappa

Theoretical MW

240 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for ABCA7 Antibody (7A1-144)

Western Blot: ABCA7 Antibody (7A1-144) [NB400-163] - Total protein from mouse platelets was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 0.5 ug/ml anti-ABCA7 in 5% non-fat milk in TBST and detected with an anti-rat HRP secondary antibody using chemiluminescence.
Western Blot: ABCA7 Antibody (7A1-144) [NB400-163] - Analysis using Azide/BSA FREE version of NB400-163. Detection of ABCA7 using NB 400-166 at various dilutions in ABCA7 transfected HeLa cell lysates.
Flow Cytometry: ABCA7 Antibody (7A1-144) [NB400-163] - Analysis using Azide/BSA FREE version of NB400-163. Analysis of 6C (red) (negative control HeLa cells) and A7(blue) (ABCA7 expressing HeLa cells) using NB 400-166 at a dilution of 1:3,000.

Applications for ABCA7 Antibody (7A1-144)

Application
Recommended Usage

Flow Cytometry

1:500 - 1:4000

Immunohistochemistry-Paraffin

1:100 - 1:250

Western Blot

1:2000
Application Notes
This ABCA7 antibody is useful for Flow Cytometry and Western Blot, where a band is observed at ~240 kDa. This ABCA7 antibody is not reactive in ELISA. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Preservative

0.05% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: ABCA7

ABCA7 (ATP-binding cassette sub-family A member 7) belongs to "A" subfamily of ABC transporters that predominantly transport lipids and other lipophilic molecules across membranes. ABCA7 shows higher expression in brain, lung, adrenal gland and spleen as well as hematopoietic tissues. It localizes to cell membrane ruffles/phagocytic cups of activated macrophages; to cytoplasm (Golgi and endosomes) of resting macrophages, and to the apical brush border of cells in the proximal tubules of kidney. It is also detected in both the plasma membrane and in intracellular compartments in myelo-lymphatic tissues (peripheral leukocytes, thymus, spleen, and bone marrow). ABCA7 facilitates the transport of phospholipids and, to a lesser extent, cholesterol, across cell membranes to extracellular apoAI and apoE discoidal lipoprotein complexes. Moreover, it implicates in macrophages mediated phagocytosis of apoptotic cells, and binds APOA1 for involving in apolipoprotein-mediated phospholipid efflux from cells. ABCA7 also mediate cholesterol efflux, and can regulate the cellular ceramide homeostasis during keratinocytes differentiation.

Alternate Names

ABCA-SSN, ABCXATP-binding cassette sub-family A member 7, ATP-binding cassette, sub-family A (ABC1), member 7, Autoantigen SS-N, EC 2.2.1.1, EC 3.6.3, EC 3.6.3.41, FLJ40025, Macrophage ABC transporter

Gene Symbol

ABCA7

Additional ABCA7 Products

Product Documents for ABCA7 Antibody (7A1-144)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for ABCA7 Antibody (7A1-144)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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