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Notch Family

Recombinant Human DLL4 Enhances BMP-2-Induced Alkaline Phosphatase Production

Enhancement of BMP-2-induced alkaline phosphatase activity by Recombinant Human DLL4 in mouse embryonic fibroblast cells

Recombinant Human DLL4 Enhances BMP-2-Induced Alkaline Phosphatase Production. The C3H10T1/2 mouse embryonic fibroblast cell line was treated with increasing concentrations of Recombinant Human DLL4 (R&D Systems, Catalog # 1506-D4), in the presence of Recombinant Human/Mouse BMP-2 (R&D Systems, Catalog # 355-BM), and alkaline phosphatase production was assessed. The ED50 for this effect is 150-600 ng/mL.

Immunostaining of DLL1 in Human Breast Cancer Tissue Using R&D Systems Mouse Anti-Human DLL1 Monoclonal Antibody

Detection of DLL1 in human breast cancer tissue using a Mouse Anti-Human DLL1 Monoclonal Antibody

Detection of DLL1 in Human Breast Cancer Tissue. DLL1 was detected in immersion-fixed, paraffin-embedded sections of human breast cancer tissue using a Mouse Anti-Human DLL1 Monoclonal Antibody (R&D Systems, Catalog # MAB18181) for 1 hour at room temperature followed by incubation with an Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (R&D Systems, Catalog # VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (R&D Systems, Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to the cytoplasm in cancer cells.

Analysis of the Binding of R&D Systems Recombinant Human Notch-4 and DLL4 Proteins

Analysis of the binding response between R&D Systems Recombinant Human Notch-4 and Recombinant Human DLL4 proteins

Notch-4 Binds to DLL4. Recombinant Human DLL4 (R&D Systems, Catalog # 1506-D4) was immobilized at 2 μg/mL and the indicated concentrations of Recombinant Human Notch-4 Fc Chimera (R&D Systems, Catalog # 9909-NT) were added. Recombinant Human Notch-4 Fc Chimera bound to Recombinant  Human DLL4 with an ED50 of 0.5-2.5 µg/mL.

Detection of Notch-1 in Rat Cortical Stem Cells Using R&D Systems Goat Anti-Mouse/Rat Notch-1 Antigen Affinity-Purified Polyclonal Antibody

Detection of Notch-1 in rat cortical stem cells using an Anti-Rat Notch-1 Antigen Affinity-Purified Polyclonal Antibody

Detection of Notch-1 in Rat Cortical Stem Cells. Notch-1 was detected in immersion-fixed undifferentiated rat cortical stem cells using a Goat Anti-Mouse/Rat Notch-1 Antigen Affinity-Purified Polyclonal Antibody (R&D Systems, Catalog # AF1057) for 3 hours at room temperature. Cells were stained using the NorthernLights™ 493-conjugated Anti-Goat IgG Secondary Antibody (R&D Systems, Catalog # NL003; green) and counterstained with DAPI (Tocris, Catalog # 5748; blue). Specific staining was localized to cell surfaces. 

Detection of Notch-2 in Rat Brain Choroid Plexus Using R&D Systems Goat Anti-Rat Notch-2 Antigen Affinity-Purified Polyclonal Antibody

Detection of Notch-2 in rat brain using a Goat Anti-Rat Notch-2 Antigen Affinity-Purified Polyclonal Antibody

Detection of Notch-2 in Rat Brain. Notch-2 was detected in perfusion-fixed frozen sections of rat brain choroid plexus using a Goat Anti-Rat Notch-2 Antigen Affinity-Purified Polyclonal Antibody (R&D Systems, Catalog # AF1190) overnight at 4˚C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit and counterstained with hematoxylin (blue).

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Optimize your stem cell culture conditions with reagents from Bio-Techne. Our catalog includes stem cell media, supplements, and proteins to promote robust cell growth wile maintaining reproducible culture conditions. Additionally, we offer stem cell differentiation and functional identification kits to simplify your workflow.

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Fluorochrome-Conjugated Antibodies for Flow Cytometry

Browse our wide selection of fluorochrome-conjugated antibodies for detecting Notch ligands and receptors by flow cytometry. We offer highly sensitive and specific antibodies conjugated to a variety of different fluorochromes to make it easy to find the right product to detect your target of interest.

Structure of small molecule for cell differentiation

Notch Inhibitors

Notch signaling regulates cell proliferation, survival, and cell fate determination during development and tumorigenesis. We offer a unique collection of potent and selective small molecule Notch inhibitors, from the Tocris brand, to explore the effects of Notch signaling in different developmental processes and pathologies.

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Hematopoietic Stem Cells Guide

Multipotent hematopoietic stem cells give rise to all of the differentiated blood and immune cell types in the body through the process of hematopoiesis. Browse our collection of tools for isolating, verifying, and differentiating HSCs toward specific immune cell lineages. We offer a range of different products to simplify the different steps of your HSC-related workflow.

Notch activation leads to cleavage and translocation of its intracellular domain to the nucleus, where it drives transcription

Growth Factor Signaling Pathways

Growth factors promote a broad range of cellular processes including proliferation, survival, differentiation, and migration and they are essential for both embryonic development and for the maintenance of tissue homeostasis in adult organisms. Explore the signaling pathways that are activated by different growth factor families and the biological effects that they mediate using our interactive signaling pathways.

Periodic Table of Human Developmental Factors Wall Poster

Periodic Table of Human Developmental Factors Wall Poster

Learn about the different families of growth factors that regulate human development with our Periodic Table of Human Developmental Factors wall poster. The poster includes information on the structures, native molecular weights, and receptors for each protein and will serve as a great reference tool to keep in your lab or office space.