rRNA Antibody (Y10b) [Allophycocyanin]
Novus Biologicals, part of Bio-Techne | Catalog # NB100-662APC
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human, Mouse, All Species, Bacteria
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Flow Cytometry, Flow (Intracellular), Immunocytochemistry, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, CyTOF-reported, CyTOF-ready
Label
Allophycocyanin (Excitation = 620-650 nm, Emission = 660-670 nm)
Antibody Source
Monoclonal Mouse IgG3 Kappa Clone # Y10b
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
The whole 5.8S ribosomal RNA.
Reactivity Notes
This antibody reacts with all eukaryotes. Bacteria reactivity reported in scientific literature (PMID: 26134566).
Localization
Cytoplasmic. Nuclear.
Specificity
This is specific for ribosomal RNA. There is no cross-reactivity with other RNAs.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG3 Kappa
Scientific Data Images for rRNA Antibody (Y10b) [Allophycocyanin]
Flow (Intracellular): rRNA Antibody (Y10b) [Allophycocyanin] [NB100-662APC] - An intracellular stain was performed on RAW 246.7 cells with rRNA Antibody (Y10b) NB100-662APC (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Allophycocyanin.
Applications for rRNA Antibody (Y10b) [Allophycocyanin]
Application
Recommended Usage
CyTOF-ready
Optimal dilutions of this antibody should be experimentally determined.
CyTOF-reported
Optimal dilutions of this antibody should be experimentally determined.
Flow (Intracellular)
Optimal dilutions of this antibody should be experimentally determined.
Flow Cytometry
Optimal dilutions of this antibody should be experimentally determined.
Immunocytochemistry
Optimal dilutions of this antibody should be experimentally determined.
Immunocytochemistry/ Immunofluorescence
Optimal dilutions of this antibody should be experimentally determined.
Immunohistochemistry
Optimal dilutions of this antibody should be experimentally determined.
Immunohistochemistry-Paraffin
Optimal dilutions of this antibody should be experimentally determined.
Immunoprecipitation
Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Optimal dilution of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS
Preservative
0.05% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C in the dark.
Background: rRNA
Alternate Names
ribosomal RNA 5.8s
Additional rRNA Products
Product Documents for rRNA Antibody (Y10b) [Allophycocyanin]
Product Specific Notices for rRNA Antibody (Y10b) [Allophycocyanin]
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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