rRNA Antibody (Y10b) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-80932

Novus Biologicals, part of Bio-Techne
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NBP2-80932
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Catalog #
BSA Free
NB100-662
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Key Product Details

Species Reactivity

Human, Mouse, All Species, Bacteria

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Flow Cytometry, Flow (Intracellular), Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, CyTOF-ready

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG3 Kappa Clone # Y10b

Format

Azide and BSA Free

Concentration

1 mg/ml

View all rRNA Antibodies »

Product Specifications

Immunogen

The whole 5.8S ribosomal RNA.

Reactivity Notes

This antibody reacts with all eukaryotes. Bacteria reactivity reported in scientific literature (PMID: 26134566).

Localization

Cytoplasmic. Nuclear.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG3 Kappa

Description

Novus Biologicals Mouse rRNA Antibody (Y10b) - Azide and BSA Free (NB100-662) is a monoclonal antibody validated for use in IHC, Flow, ICC/IF and IP. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for rRNA Antibody (Y10b) - Azide and BSA Free

Immunocytochemistry/ Immunofluorescence: rRNA Antibody (Y10b) - Azide and BSA Free [NBP2-80932]

Immunocytochemistry/ Immunofluorescence: rRNA Antibody (Y10b) - Azide and BSA Free [NBP2-80932]

Immunocytochemistry/Immunofluorescence: rRNA Antibody (Y10b) - Azide and BSA Free [NBP2-80932] - Staining of rRNA in vero cells. Image from the standard format of this antibody.
Immunohistochemistry: rRNA Antibody (Y10b) - Azide and BSA Free [NBP2-80932]

Immunohistochemistry: rRNA Antibody (Y10b) - Azide and BSA Free [NBP2-80932]

Immunohistochemistry: rRNA Antibody (Y10b) - Azide and BSA Free [NBP2-80932] - Immunohistochemical staining of mouse neuronal progenitor culture using rRNA antibody (green). Image from verified customer review. Image from the standard format of this antibody.
Flow Cytometry: rRNA Antibody (Y10b) - Azide and BSA Free [NBP2-80932]

Flow Cytometry: rRNA Antibody (Y10b) - Azide and BSA Free [NBP2-80932]

Flow Cytometry: rRNA Antibody (Y10b) - Azide and BSA Free [NBP2-80932] - An intracellular stain was performed on HeLa cells with rRNA Antibody [Y10b] NB100-662B (blue) and a matched isotype control (orange). Both antibodies were directly conjugated to Biotin. Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature, followed by Streptavidin - R-Phycoerythrin Protein (2012-1000, Novus Biologicals).
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Applications for rRNA Antibody (Y10b) - Azide and BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:2500

Immunoprecipitation

10 ul/lysate
Application Notes
This rRNA Antibody (Y10b) is useful for Immunocytochemistry/Immunofluorescence and Immunoprecipitation. In ICC/IF, staining was observed in Ribosomes in the nucleoli and cytoplasm of Ntera2 cells.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: rRNA

rRNA (ribosomal RNA) forms a major structural component of ribosomes which provides a mechanism for decoding mRNA information into amino acids/proteins through the process of translation. rRNA plays a key role in accurate positioning of mRNA as well as peptidyl tRNA, and also plays a structural role in peptidyl transferase activity by generating scaffolds where ribosomal proteins are assembled. rRNA is generated inside nucleolus, which is localized in nucleus of the cells and contains genes essential for encoding rRNA. Defective rRNA may implicate in certain pathologies, for example: decreased rRNA expression has been observed in subjects with myelodysplastic syndromes, and abnormal rRNA expression has also been linked to Alzheimer's disease. Our rRNA (Y10b) antibody recognizes both the large as well as the small subunit of rRNA from all eukaryotic cells and with is applicability in ICC/IF and IP techniques, it provides a valuable molecular tool for rRNA research.

Alternate Names

ribosomal RNA 5.8s

Additional rRNA Products

Product Documents for rRNA Antibody (Y10b) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

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Product Specific Notices for rRNA Antibody (Y10b) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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