rRNA Antibody (Y10b) - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NB100-662

Novus Biologicals, part of Bio-Techne
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NB100-662SS
NB100-662
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, All Species, Bacteria

Cited:

Human, Mouse, Rat, Avian - Chicken, Fish, Other, Plant

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Flow Cytometry, Flow (Intracellular), Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, CyTOF-reported, CyTOF-ready

Cited:

Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Chromatin Immunoprecipitation (ChIP), IF/IHC

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG3 Kappa Clone # Y10b

Format

BSA Free

Concentration

1.0 mg/ml

View all rRNA Antibodies »

Product Specifications

Immunogen

The whole 5.8S ribosomal RNA.

Reactivity Notes

This antibody reacts with all eukaryotes. Bacteria reactivity reported in scientific literature (PMID: 26134566).

Localization

Cytoplasmic. Nuclear.

Specificity

This is specific for ribosomal RNA. There is no cross-reactivity with other RNAs.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG3 Kappa

Description

Novus Biologicals Mouse rRNA Antibody (Y10b) - BSA Free (NB100-662) is a monoclonal antibody validated for use in IHC, WB, Flow, ICC/IF, IP and ChIP. Anti-rRNA Antibody: Cited in 31 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for rRNA Antibody (Y10b) - BSA Free

Immunocytochemistry/ Immunofluorescence: rRNA Antibody (Y10b) - BSA Free [NB100-662]

Immunocytochemistry/ Immunofluorescence: rRNA Antibody (Y10b) - BSA Free [NB100-662]

Immunocytochemistry/Immunofluorescence: rRNA Antibody (Y10b) [NB100-662] - HeLa cells were fixed and permeabilized for 10 minutes with -20C MeOH. The cells were incubated with rRNA Antibody [Y10b] conjugated to Biotin (NB100-662B) at 5ug/ml overnight at 4C and detected with Streptavidin conjugated to DyLight 550. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.
Flow Cytometry: rRNA Antibody (Y10b) - BSA Free [NB100-662]

Flow Cytometry: rRNA Antibody (Y10b) - BSA Free [NB100-662]

Flow Cytometry: rRNA Antibody (Y10b) [NB100-662] - An intracellular stain was performed on HeLa cells with rRNA Antibody [Y10b] NB100-662B (blue) and a matched isotype control (orange). Both antibodies were conjugated to Biotin. Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature, followed by Streptavidin - R-Phycoerythrin Protein (2012-1000, Novus Biologicals).
Immunocytochemistry/ Immunofluorescence: rRNA Antibody (Y10b) - BSA Free [NB100-662]

Immunocytochemistry/ Immunofluorescence: rRNA Antibody (Y10b) - BSA Free [NB100-662]

Immunocytochemistry/Immunofluorescence: rRNA Antibody (Y10b) [NB100-662] - rRNA antibody was tested in Ntera-2 cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and DyLight 550 (red).
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Applications for rRNA Antibody (Y10b) - BSA Free

Application
Recommended Usage

CyTOF-ready

reported by customer review

Flow (Intracellular)

2.5 ug/ml

Flow Cytometry

2.5 ug/ml

Immunocytochemistry/ Immunofluorescence

reported in scientific literature (PMID 34980897)

Immunohistochemistry

1:100 - 1:300

Immunohistochemistry-Paraffin

1:100 - 1:300

Immunoprecipitation

10 ul/lysate
Application Notes
In ICC/IF, staining was observed in Ribosomes in the nucleoli and cytoplasm of Ntera2 cells.

Reviewed Applications

Read 2 reviews rated 5 using NB100-662 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: rRNA

rRNA (ribosomal RNA) forms a major structural component of ribosomes which provides a mechanism for decoding mRNA information into amino acids/proteins through the process of translation. rRNA plays a key role in accurate positioning of mRNA as well as peptidyl tRNA, and also plays a structural role in peptidyl transferase activity by generating scaffolds where ribosomal proteins are assembled. rRNA is generated inside nucleolus, which is localized in nucleus of the cells and contains genes essential for encoding rRNA. Defective rRNA may implicate in certain pathologies, for example: decreased rRNA expression has been observed in subjects with myelodysplastic syndromes, and abnormal rRNA expression has also been linked to Alzheimer's disease. Our rRNA (Y10b) antibody recognizes both the large as well as the small subunit of rRNA from all eukaryotic cells and with is applicability in ICC/IF and IP techniques, it provides a valuable molecular tool for rRNA research.

Alternate Names

ribosomal RNA 5.8s

Additional rRNA Products

Product Documents for rRNA Antibody (Y10b) - BSA Free

Product Datasheets

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Product Specific Notices for rRNA Antibody (Y10b) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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