rRNA Antibody (Y10b) [Biotin]
Novus Biologicals, part of Bio-Techne | Catalog # NB100-662B
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human, Mouse, All Species, Bacteria
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Flow Cytometry, Flow (Intracellular), Immunocytochemistry, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, CyTOF-reported, CyTOF-ready
Label
Biotin
Antibody Source
Monoclonal Mouse IgG3 Kappa Clone # Y10b
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
The whole 5.8S ribosomal RNA.
Reactivity Notes
This antibody reacts with all eukaryotes. Bacteria reactivity reported in scientific literature (PMID: 26134566).
Localization
Cytoplasmic. Nuclear.
Specificity
This is specific for ribosomal RNA. There is no cross-reactivity with other RNAs.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG3 Kappa
Scientific Data Images for rRNA Antibody (Y10b) [Biotin]
Immunocytochemistry/Immunofluorescence: rRNA Antibody (Y10b) [Biotin] [NB100-662B] - HeLa cells were fixed and permeabilized for 10 minutes with -20C MeOH. The cells were incubated with rRNA Antibody [Y10b] conjugated to Biotin (NB100-662B) at 5ug/ml overnight at 4C and detected with Streptavidin conjugated to DyLight 550. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.
Flow Cytometry: rRNA Antibody (Y10b) [Biotin] [NB100-662B] - An intracellular stain was performed on HeLa cells with rRNA Antibody [Y10b] NB100-662B (blue) and a matched isotype control (orange). Both antibodies were conjugated to Biotin. Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature, followed by Streptavidin - R-Phycoerythrin Protein (2012-1000, Novus Biologicals).
![rRNA Antibody (Y10b) [Biotin]](https://resources.bio-techne.com/images/products/nb100-662b_mouse-monoclonal-rrna-antibody-y10b-biotin-immunocytochemistry-immunofluorescence-31122024171610..jpg "rRNA (Yb10) in NIH-3T3 Mouse Cell Line.")
rRNA (Yb10) in NIH-3T3 Mouse Cell Line.
rRNA (Yb10) was detected in immersion fixed NIH3T3 Mouse fibroblast cell line using Mouse anti- rRNA (Yb10) Protein G Purified Monoclonal Antibody conjugated to Biotin (Catalog # NB100-662B) at 5 µg/mL overnight at 4C. Cells were stained using Streptavidin conjugated to DyLight 550 (red) and counterstained with DAPI (blue). Cells were imaged using a 100X objective and digitally deconvolved.Applications for rRNA Antibody (Y10b) [Biotin]
Application
Recommended Usage
CyTOF-ready
Optimal dilutions of this antibody should be experimentally determined.
CyTOF-reported
Optimal dilutions of this antibody should be experimentally determined.
Flow (Intracellular)
Optimal dilutions of this antibody should be experimentally determined.
Flow Cytometry
Optimal dilutions of this antibody should be experimentally determined.
Immunocytochemistry
Optimal dilutions of this antibody should be experimentally determined.
Immunocytochemistry/ Immunofluorescence
Optimal dilutions of this antibody should be experimentally determined.
Immunohistochemistry
Optimal dilutions of this antibody should be experimentally determined.
Immunohistochemistry-Paraffin
Optimal dilutions of this antibody should be experimentally determined.
Immunoprecipitation
Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Optimal dilution of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS
Preservative
0.05% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C in the dark.
Background: rRNA
Alternate Names
ribosomal RNA 5.8s
Additional rRNA Products
Product Documents for rRNA Antibody (Y10b) [Biotin]
Product Specific Notices for rRNA Antibody (Y10b) [Biotin]
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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