Why use Pre-isolated Fully Characterized Mouse Cortical Stem Cells?
Although there are a number of protocols available to isolate mouse cortical stem cells, each isolation can yield cells that vary in their purity and ability to proliferate. See Details
R&D Systems offers Mouse Cortical Stem Cells that are of high purity, isolated at a low passage number (Passage 1), and are free of mycoplasma and microbial contamination. Mouse primary cortical stem cells were isolated from the cortex of embryonic E14.5 CD-1 mice. Cells were cultured as a monolayer in media containing the N-2 Plus Media Supplement (Catalog # AR003) or N-2 MAX Media Supplement (Catalog # AR009), Recombinant Human FGF basic (Catalog # 233-FB or 4114-TC), and Recombinant Human EGF (Catalog # 236-EG). The cells were passaged once and the expanded progeny were cryopreserved. These cells are designated as passage 1 (P1) cells.
R&D Systems Rat Cortical Stem Cells:
Have been fully characterized to reduce experimental variation.
Differentiate into neurons, astrocytes, and oligodendrocytes.
Have been verified to be free of mycoplasma and microbial contamination.
2 vials of Mouse Cortical Stem Cells containing 2 x 106 cells. See Details
Stability and Storage
Store Mouse Cortical Stem Cells in liquid nitrogen for up to one year.
The Mouse Cortical Stem Cells have been thawed and tested for their ability to proliferate using either a monolayer system (for up to 3 passages) or a neurosphere system (for up to 4 passages). Stem and progenitor cells expanded from the end of passage 3 (monolayer system) or passage 4 (neurosphere system) have been examined for Nestin and SOX2 expression as well as for their capacity to differentiate into astrocytes, neurons, and oligodendrocytes.
The cells tested negative for mycoplasma using the MycoProbe™ Mycoplasma Detection Kit (Catalog # CUL001B). The cells also tested negative for microbial contamination.
Characterization of Mouse Cortical Stem Cells. A. Undifferentiated Mouse Cortical Stem Cells (Catalog # NSC002) cultured on Poly-L-ornithine and Fibronectin coated dishes. B. Mouse Cortical Stem Cells express Nestin and SOX2, a phenotype that is consistent with mouse cortical stem cells. Mouse Cortical Stem Cells were stained with a PE-conjugated Mouse Anti-Mouse/Rat Nestin Monoclonal Antibody (Catalog # IC2736P; red histogram), a PE-conjugated Mouse Anti-Human/Mouse SOX2 Monoclonal Antibody (Catalog # IC2018P; green histogram), or a PE-conjugated Mouse IgG2A Isotype Control Antibody (Catalog # IC003P; empty histogram).
Mouse Cortical Stem Cells Have Multi-lineage Potential. A-C Differentiation of Mouse Cortical Stem Cells (Catalog # NSC002) was induced by culturing the cells in DMEM/F12 containing N2-Plus Media Supplement (Catalog # AR003) in the absence of EGF and FGF basic for 7 days. Neuronal lineage cells (A) were detected with a Mouse Anti-Neuron Specific beta-III Tubulin Monoclonal Antibody (Catalog # MAB1195) followed by the NorthernLights™ (NL)557-conjugated Donkey Anti-Mouse IgG Secondary Antibody (Catalog # NL007; red). Astrocyte lineage cells (B) were detected with a Sheep Anti-Human GFAP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2594) followed by the NL557-conjugated Donkey Anti-Sheep IgG Secondary Antibody (Catalog # NL010; red). Oligodendrocyte lineage cells (C) were detected with a Mouse Anti-Oligodendrocyte Marker O4 Monoclonal Antibody (Catalog # MAB1326) followed by the NL557-conjugated Goat Anti-Mouse IgM Secondary Antibody (Catalog # NL019; red). The nuclei were counterstained with DAPI (blue) in all panels. View our protocol for Fluorescent ICC Staining of Stem Cells on Coverslips.
Neural stem cells provide an excellent model for research focused on neural development and neurological disorders. R&D Systems offers ready-to-use primary cortical stem cells isolated from E14.5 Sprague-Dawley rats. In addition, primary mouse cortical stem cells isolated from E14.5 CD-1 mice are available. Every lot of R&D Systems Cortical Stem Cells is validated for a high level of Nestin expression and the capacity for multi-lineage differentiation into astrocytes, neurons, and oligodendrocytes. Our cortical stem cells are tested to ensure highest quality and lot to lot consistency. Both rat and mouse Cortical Stem Cells can be optimally expanded as monolayers or neurospheres.
To complement the use of primary neural stem cells, we offer a range of supportive products, including culture media which is specifically optimized for use with neural stem cells. We offer kits to promote the in vitro proliferation of neural precursors, and kits to differentiate neural stem cells into dopaminergic neurons or oligodendrocytes. In addition, kits are available which contain panels of antibodies designed to monitor the differentiation and identification of neural precursors, astrocytes, neurons, and oligodendrocytes.
Preparation & Storage
The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Store in liquid nitrogen for up to 12 months from the date of receipt.
Refer to the product datasheet for complete product details.
Briefly, Mouse Cortical Stem Cells can be thawed and expanded by the neurosphere or monolayer system using the following procedure:
Thaw the Mouse Cortical Stem Cells
Plate the cells in Completed NSC Base Media containing EGF and FGF basic
Expand the cells using the monolayer or neurosphere system
How many times can Mouse Cortical Stem Cells (Catalog # NSC002) be passaged before they lose their multipotency?
The Mouse Cortical Stem Cells are designated P1 cells as the supplied cells are passaged once. They can be expanded at least three more passages using the monolayer system or at least four more passages using the neurosphere system before their multipotency may be compromised.
What is the gender of the mice from which Mouse Cortical Stem Cells are derived?
Mouse cortical Stem cells are deived by harvesting brain tissue from embryos of pregnant females.Cortical Stem cells from each litter is combined giving a mixture of male and female cells.
Which Brands are Currently Available on bio‑techne.com? R&D Systems, Tocris Bioscience and ProteinSimple branded products are available to purchase through bio‑techne.com. ProteinSimple branded instruments and Simple Plex Assays are available to quote. ProteinSimple branded instrument consumables and ACD branded products will be available on bio‑techne.com in the near future. Novus Biologicals branded products are not currently available on bio‑techne.com and can be found at novusbio.com.
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