Wnt-C59

Tocris Bioscience, part of Bio-Techne | Catalog # 5148

Highly potent PORCN inhibitor
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Key Product Details

Description

Highly potent PORCN inhibitor

View all PORCN Inhibitors »

Product Description

Wnt-C59 is a highly potent inhibitor of the MBOAT (membrane-bound O-acyltranferase) family member, Porcupine (PORCN) (IC50 = 74 pM), that mediates WNT palmitoylation and secretion. Wnt-C59 potently inhibits the processing of both canonical (1, 2, 3a, 6, 7b, 8a, 9a, 9b, 10) and non-canonical (4, 5a, 11, 16) Wnt subtypes. Wnt-C59 blocks progression of mammary tumors in MMTV-WNT1 transgenic mice and downregulates Wnt/β-catenin target genes. Wnt-C59-treated tumors show a decrease in β-catenin, CyclinD1 and c-Myc. Wnt-C59 induces cardiomyocyte differentiation from human iPSCs following culture with CHIR 99021 (Cat. No. 4423). Wnt-C59 efficiently induces neural differentiation of CTIP2+/COUP-TF1- cells from PSCs in culture. When grafted into the cortex of adult mice, Wnt-C59-treated cells develop abundant axonal fiber extensions toward the spinal cord. The compound has also been used in protocols to generate β cells from human PSCs. Cell permeable and orally bioavailable.

Product Specifications

Molecular Weight

379.45

Formula

C25H21N3O

Storage

Store at -20°C

Purity

≥99% (HPLC)

Chemical Name

4-(2-Methyl-4-pyridinyl)-N-[4-(3-pyridinyl)phenyl]benzeneacetamide

CAS Number

1243243-89-1

PubChem ID

57519544

InChI Key

KHZOJCQBHJUJFY-UHFFFAOYSA-N

SMILES

O=C(CC3=CC=C(C4=CC=NC(C)=C4)C=C3)NC1=CC=C(C2=CC=CN=C2)C=C1

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Solubility

Solvent Max Conc. mg/mL Max Conc. mM
Solubility
DMSO 7.59 20
Ethanol 7.59 20

Preparing Stock Solutions for Wnt-C59

The following data is based on the product molecular weight 379.45

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which all affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:

Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
0.2 mM 13.18 mL 65.88 mL 131.77 mL
1 mM 2.64 mL 13.18 mL 26.35 mL
2 mM 1.32 mL 6.59 mL 13.18 mL
10 mM 0.26 mL 1.32 mL 2.64 mL

Calculators

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Product Documents for Wnt-C59

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for Wnt-C59

For research use only

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