SAG

Product Description

SAG is a potent Smoothened (Smo) receptor agonist (K_d = 59 nM); antagonizes Cyclopamine (Cat. No. 1623) action at the Smo receptor. SAG potently activates the Hedgehog signaling pathway in Shh-light 2 cells (EC₅₀ ~ 3 nM) and induces pathway activation independently of Ptch proteins. SAG is a putative inhibitor of a cellular component required for Hedgehog signaling and also enhances neuronal differentiation of iPSCs into dopaminergic neurons.

SAG dihydrochloride (Cat. No. 6390) also available.

Solubility

Preparing Stock Solutions for SAG

The following data is based on the product molecular weight 490.06

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which all affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:

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Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
1 mM	2.04 mL	10.20 mL	20.41 mL
5 mM	0.41 mL	2.04 mL	4.08 mL
10 mM	0.20 mL	1.02 mL	2.04 mL
50 mM	0.04 mL	0.20 mL	0.41 mL

Calculators

Background References

References are publications that support the biological activity of the product.

• Chen Small molecule modulation of Smoothened activity. Proc.Natl.Acad.Sci.USA. 2002 PMID: 12391318
• Mak Small molecules greatly improve conversion of human-induced pluripotent stem cells to the neuronal lineage. Stem Cells Int. 2012 PMID: 22567022
• Stanton A small molecule that binds Hedgehog and blocks its signaling in human cells. Nat.Chem.Biol. 2009 PMID: 19151731