SAG

Name: SAG
Brand: Tocris Bioscience
SKU: 4366
Rating: 5 (2 reviews)

Tocris Bioscience, part of Bio-Techne | Catalog # 4366

(2)

Citations (60)

COA / SDS

Potent Smoothened receptor agonist; activates the Hedgehog signaling pathway

Key Product Details

Description:	Potent Smoothened receptor agonist; activates the Hedgehog signaling pathway
Chemical Name:	3-Chloro-N-[trans-4-(methylamino)cyclohexyl]-N-[[3-(4-pyridinyl)phenyl]methyl]benzo[b]thiophene-2-carboxamide
Purity:	≥98% (HPLC)
Molecular Weight:	490.06

View Full Technical Details for SAG

Technical Data
Citations / Reviews (2)

Biological Activity for SAG

SAG is a potent Smoothened (Smo) receptor agonist (K_d = 59 nM); antagonizes Cyclopamine (Cat. No. 1623) action at the Smo receptor. SAG potently activates the Hedgehog signaling pathway in Shh-light 2 cells (EC₅₀ ~ 3 nM) and induces pathway activation independently of Ptch proteins. SAG is a putative inhibitor of a cellular component required for Hedgehog signaling and also enhances neuronal differentiation of iPSCs into dopaminergic neurons.

SAG dihydrochloride (Cat. No. 6390) also available.

Compound Libraries for SAG

SAG is also offered as part of the Tocriscreen Stem Cell Library. Find out more about compound libraries available from Tocris.

Technical Data for SAG

M. Wt	490.06
Formula	C₂₈H₂₈ClN₃OS
Storage	Store at -20°C
Purity	≥98% (HPLC)
CAS Number	912545-86-9
PubChem ID	5284330
InChi Key	VFSUUTYAEQOIMW-UHFFFAOYSA-N
Smiles	ClC2=C(C(N([C@H]4CC[C@H](NC)CC4)CC3=CC=CC(C5=CC=NC=C5)=C3)=O)SC1=CC=CC=C12

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility for SAG

	Solvent	Max Conc. mg/mL	Max Conc. mM
Solubility
Solubility	DMSO	49.01	100

Preparing Stock Solutions for SAG

The following data is based on the product molecular weight 490.06.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
1 mM	2.04 mL	10.20 mL	20.41 mL
5 mM	0.41 mL	2.04 mL	4.08 mL
10 mM	0.20 mL	1.02 mL	2.04 mL
50 mM	0.04 mL	0.20 mL	0.41 mL

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Reconstitution Calculator

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References for SAG

References are publications that support the biological activity of the product.

Chen Small molecule modulation of Smoothened activity. Proc.Natl.Acad.Sci.USA. 2002 PMID: 12391318
Mak Small molecules greatly improve conversion of human-induced pluripotent stem cells to the neuronal lineage. Stem Cells Int. 2012 PMID: 22567022
Stanton A small molecule that binds Hedgehog and blocks its signaling in human cells. Nat.Chem.Biol. 2009 PMID: 19151731

SAG