SAG

Tocris Bioscience a Bio-Techne Brand

Catalog # 4366

Citations (5)

(2)

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Key Product Details

Description:	Potent Smoothened receptor agonist; activates the Hedgehog signaling pathway
Chemical Name:	3-Chloro-N-[trans-4-(methylamino)cyclohexyl]-N-[[3-(4-pyridinyl)phenyl]methyl]benzo[b]thiophene-2-carboxamide
Purity:	≥98% (HPLC)
Molecular Weight:	490.06

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Biological Activity for SAG

SAG is a potent Smoothened (Smo) receptor agonist (K_d = 59 nM); antagonizes Cyclopamine (Cat. No. 1623) action at the Smo receptor. SAG potently activates the Hedgehog signaling pathway in Shh-light 2 cells (EC₅₀ ~ 3 nM) and nduces pathway activation independently of Ptch proteins. SAG is a putative inhibitor of a cellular component required for Hedgehog signaling and also enhances neuronal differentiation of iPSCs into dopaminergic neurons.

SAG dihydrochloride (Cat. No. 6390) also available.

Compound Libraries for SAG

SAG is also offered as part of the Tocriscreen Stem Cell Library. Find out more about compound libraries available from Tocris.

Technical Data for SAG

M. Wt	490.06
Formula	C₂₈H₂₈ClN₃OS
Storage	Store at -20°C
Purity	≥98% (HPLC)
CAS Number	912545-86-9
PubChem ID	5284330
InChi Key	VFSUUTYAEQOIMW-UHFFFAOYSA-N
Smiles	ClC2=C(C(N([C@H]4CC[C@H](NC)CC4)CC3=CC=CC(C5=CC=NC=C5)=C3)=O)SC1=CC=CC=C12

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility for SAG

	Solvent	Max Conc. mg/mL	Max Conc. mM
Solubility
Solubility	DMSO	49.01	100

Preparing Stock Solutions for SAG

The following data is based on the product molecular weight 490.06.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
1 mM	2.04 mL	10.20 mL	20.41 mL
5 mM	0.41 mL	2.04 mL	4.08 mL
10 mM	0.20 mL	1.02 mL	2.04 mL
50 mM	0.04 mL	0.20 mL	0.41 mL

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References for SAG

References are publications that support the biological activity of the product.

Mak Small molecules greatly improve conversion of human-induced pluripotent stem cells to the neuronal lineage. Stem Cells Int. 2012 PMID: 22567022
Stanton A small molecule that binds Hedgehog and blocks its signaling in human cells. Nat.Chem.Biol. 2009 PMID: 19151731
Chen Small molecule modulation of Smoothened activity. Proc.Natl.Acad.Sci.USA. 2002 PMID: 12391318

Citations for SAG (5)

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Citations are publications that use Bio-Techne products. Selected citations for SAG include:

Haidar et al. (2019) Neuropathy-causing mutations in HSPB1 impair autophagy by disturbing the formation of SQSTM1/p62 bodies. Autophagy. 15:1051
PMID: 30669930
Atzmon et al. (2018) Drug Screening Identifies Sigma-1-Receptor as a Target for the Therapy of VWM Leukodystrophy. Front Mol Neurosci. 11:336
PMID: 30279648
Rajamani et al. (2018) Super-Obese Patient-Derived iPSC Hypothalamic Neurons Exhibit Obesogenic Signatures and Hormone Responses. Cell Stem Cell. 22:698
PMID: 29681516
Xia et al. (2016) Transcriptional comparison of human induced and primary midbrain DArgic neurons. Sci.Rep.. 6:20270
PMID: 26842779
Cortes et al. (2015) Accumulation of the Vitamin D Precursor Cholecalciferol Antagonizes Hedgehog Signaling to Impair Hemogenic Endothelium Formation. Nat Chem Biol. 5:471
PMID: 26365513

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Customer Reviews for SAG (2)

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Promoted the formation of cilia

Name: Kishor Pant

Species: Human

Assay Type: In Vitro

Cell Line/Tissue: HepG2, and Huh,

SAG consistently produced positive results in our experiments; it promoted the formation of cilia and reduced the proliferation of cancer cells.

We use 5-20 nm concentration for 24-48 hours.
Usage Information

Name: Anonymous

Species: Mouse

Assay Type: In Vitro

Cell Line/Tissue: iPSC

iPSC-derived hypothalamic neuron differentiation activation by Smoothened agonist SAG 1 μM and purmorphamine 1 μM.

From Day 2 to day 9 to direct the cells toward ventral diencephalon with regular media change every 2 days.

PMID: 29681516

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Product Documents for SAG

COA

Certificate of Analysis

To download a Certificate of Analysis, please select a batch below.

SAG