SAG dihydrochloride

Name: SAG dihydrochloride
Brand: Tocris Bioscience
SKU: 6390
Rating: 5 (1 reviews)

Tocris Bioscience a Bio-Techne Brand

Catalog # 6390

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Key Product Details

Description:	Dihydrochloride salt of SAG; Smo receptor agonist
Chemical Name:	3-Chloro-N-[trans-4-(methylamino)cyclohexyl]-N-[[3-(4-pyridinyl)phenyl]methyl]benzo[b]thiophene-2-carboxamide dihydrochloride
Purity:	≥98% (HPLC)
Molecular Weight:	562.98

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Biological Activity for SAG dihydrochloride

SAG dihydrochloride is a water-soluble dihydrochloride salt of SAG (Cat. No. 4366). SAG is a potent Smoothened (Smo) receptor agonist (Kd = 59 nM); antagonizes Cyclopamine (Cat. No. 1623) action at the Smo receptor. SAG potently activates the Hedgehog signaling pathway in Shh-light 2 cells (EC₅₀ ~ 3 nM) and induces pathway activation independently of Ptch proteins. SAG is a putative inhibitor of a cellular component required for Hedgehog signaling and also enhances neuronal differentiation of iPSCs into dopaminergic neurons. The compound can be used in protocols for the chemical reprogramming of somatic cells to iPSCs.

Technical Data for SAG dihydrochloride

M. Wt	562.98
Formula	C₂₈H₂₈ClN₃OS.2HCl
Storage	Store at -20°C
Purity	≥98% (HPLC)
PubChem ID	71433770
InChi Key	SWBYSYANHQTINY-CZBZHNTRSA-N
Smiles	ClC1=C(C(N([C@H]2CC[C@H](NC)CC2)CC3=CC=CC(C4=CC=NC=C4)=C3)=O)SC5=CC=CC=C51.Cl.Cl

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility for SAG dihydrochloride

	Solvent	Max Conc. mg/mL	Max Conc. mM
Solubility
	water	56.3	100
	DMSO	56.3	100

Preparing Stock Solutions for SAG dihydrochloride

The following data is based on the product molecular weight 562.98.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
1 mM	1.78 mL	8.88 mL	17.76 mL
5 mM	0.36 mL	1.78 mL	3.55 mL
10 mM	0.18 mL	0.89 mL	1.78 mL
50 mM	0.04 mL	0.18 mL	0.36 mL

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References for SAG dihydrochloride

References are publications that support the biological activity of the product.

Guan Chemical reprogramming of human somatic cells to pluripotent stem cells. Nature 2022 PMID: 35418683

Citations for SAG dihydrochloride

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Customer Reviews for SAG dihydrochloride (1)

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MUCH better than the others!!

Name: Anonymous

Species: Human

Assay Type: In Vitro

Cell Line/Tissue: stem cells

This SAG performs the BEST for stem cell differentiation to neuronal lineages. Other brands do not work. We use it at 1uM after dual SMAD inhibition.

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