Minocycline hydrochloride

Name: Minocycline hydrochloride
Brand: Tocris Bioscience
SKU: 3268
Rating: 5 (1 reviews)

Tocris Bioscience a Bio-Techne Brand

Catalog # 3268

Citations (2)

(1)

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Key Product Details

Description:	Antibiotic. Displays neuroprotective effects
Chemical Name:	[4S-(4α,4aα,5aα,12aα)]-4,7-Bis(dimethylamino)-1,4,4a,5,5a,6,11,12a-octahydro-3,10,12,12a,tetrahydroxy-1,11-dioxo-2-naphthacenecarboxamide
Purity:	≥98% (HPLC)
Molecular Weight:	493.94

View Full Technical Details for Minocycline hydrochloride

Biological Activity for Minocycline hydrochloride

Minocycline hydrochloride is a tetracycline antibiotic. Displays neuroprotective, antiapoptotic, anti-inflammatory and antimicrobial effects. Acts as a matrix metalloproteinase (MMP) inhibitor; attenuates disease severity in mouse models of multiple sclerosis. Orally active and brain penetrant. Allows formation of extended pluripotent stem (EPS) cells in combination with CHIR 99021 (Cat.No. 4423), (S)-(+)-Dimethindene maleate (Cat.No. 1425) and human leukemia inhibitory factor.

For more information about how Minocycline hydrochloride may be used, see our protocol: Generation and Propagation of EPS cells (LCDM Cocktail).

Technical Data for Minocycline hydrochloride

M. Wt	493.94
Formula	C₂₃H₂₇N₃O₇.HCl
Storage	Store at +4°C
Purity	≥98% (HPLC)
CAS Number	13614-98-7
PubChem ID	54685925
InChi Key	WTJXVDPDEQKTCV-VQAITOIOSA-N
Smiles	NC(C4=C(O)[C@@H](N(C)C)[C@@]3([H])[C@](C4=O)(O)C(O)=C2C(C1=C(O)C=CC(N(C)C)=C1C[C@]([H])2C3)=O)=O.Cl

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility for Minocycline hydrochloride

	Solvent	Max Conc. mg/mL	Max Conc. mM
Solubility
	DMSO	49.39	100
	water	12.35	25

Preparing Stock Solutions for Minocycline hydrochloride

The following data is based on the product molecular weight 493.94.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
1 mM	2.02 mL	10.12 mL	20.25 mL
5 mM	0.40 mL	2.02 mL	4.05 mL
10 mM	0.20 mL	1.01 mL	2.02 mL
50 mM	0.04 mL	0.20 mL	0.40 mL

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References for Minocycline hydrochloride

References are publications that support the biological activity of the product.

Yang Derivation of pluripotent stem cells with in vivo embryonic and extraembryonic potency. Cell. 2017 PMID: 28388409
Padi and Kulkarni MinCyc prevents the development of neuropathic pain, but not acute pain: possible anti-inflammatory and antioxidant mechanisms. Eur.J.Pharmacol. 2008 PMID: 18952075
Brundula Targeting leukocyte MMPs and transmigration: MinCyc as a potential therapy for multiple sclerosis. Brain 2002 PMID: 12023318
Tikka MinCyc, a tetracy. derivative, is neuroprotective against excitotoxicity by inhibiting activation and proliferation of microglia. J.Neurosci. 2001 PMID: 11306611

Citations for Minocycline hydrochloride (2)

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Citations are publications that use Bio-Techne products. Selected citations for Minocycline hydrochloride include:

Yaeger and Trussell (2015) Single granule cells excite Golgi cells and evoke feedback inhibition in the cochlear nucleus. J Neurosci. 35:4741
PMID: 25788690
Song et al. (2015) MinCyc does not affect long-term potentiation in the anterior cingulate cortex of normal adult mice. Hum Mol Genet. 11:25
PMID: 25933605

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Customer Reviews for Minocycline hydrochloride (1)

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Blocker of microglia reactivity

Name: Luiz Alexandre Magno

Species: Mouse

Assay Type: In Vivo

Cell Line/Tissue: Brain

I have used the antibiotic minocycline to impair activation of microglia in the brain of mice. This drug has anti-inflammatory properties that block microglial reactivity, which can be easily confirmed by expression of the CD68 protein. The drug was diluted in PBS and injected i.p. (75 mg/kg) in mice, twice a day, for 7 days. The image shows that microglial cells (stained for Iba1) do not express CD68 (in red) after treatment with minocycline.

PMID: 17376966

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Product Documents for Minocycline hydrochloride

COA

Certificate of Analysis

To download a Certificate of Analysis, please select a batch below.

SDS

⚠ WARNING: This product can expose you to chemicals including Minocycline hydrochloride (internal use), which is known to the State of California to cause reproductive toxicity with developmental effects. For more information, go to www.P65Warnings.ca.gov