MD13

Name: MD13
Brand: Tocris Bioscience
SKU: 7503

Tocris Bioscience, part of Bio-Techne | Catalog # 7503

COA / SDS

Potent MIF Degrader (PROTAC®)

Key Product Details

Description:	Potent MIF Degrader (PROTAC^®)
Chemical Name:	8-((2-(2,6-Dioxopiperidin-3-yl)-1,3-dioxoisoindolin-4-yl)amino)-N-(4-(7-hydroxy-2-oxo-2H-benzo[e][1,3]oxazin-3(4H)-yl)phenyl)octanamide
Purity:	≥98% (HPLC)
Molecular Weight:	653.69

View Full Technical Details for MD13

Technical Data
Citations

Biological Activity for MD13

MD13 is a potent macrophage migration inhibitory factor (MIF) Degrader (PROTAC^®; DC₅₀ < 100 nM; D_max = 92% after 6 h). MD13 comprises the cereblon ligand Pomalidomide (Cat. No. 6302) joined by a linker to a MIF inhibitor. MD13 reduces MIF levels, arrests cells at the G₂/M phase and inhibits proliferation of A549 lung carcinoma cells. Growth of A549 cell 3D spheroids is also inhibited by MD13.

Negative control MD15 (Cat. No. 7504) and MIF antibodies validated for Simple Western™ (automated Western) instruments and Western Blot also available: Catalog # AF-289-PB and MAB289.

PROTAC^® is a registered trademark of Arvinas Operations, Inc., and is used under license.

Licensing Information

Sold with kind permission of Professor Dekker.

Technical Data for MD13

M. Wt	653.69
Formula	C₃₅H₃₅N₅O₈
Storage	Store at -20°C
Purity	≥98% (HPLC)
CAS Number	2758431-97-7
InChi Key	HAHDZDUOFHMMEA-UHFFFAOYSA-N
Smiles	O=C1N(CC2=CC=C(C=C2O1)O)C3=CC=C(C=C3)NC(CCCCCCCNC4=C(C5=CC=C4)C(N(C5=O)C6CCC(NC6=O)=O)=O)=O

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility for MD13

	Solvent	Max Conc. mg/mL	Max Conc. mM
Solubility
Solubility	DMSO	65.37	100

Preparing Stock Solutions for MD13

The following data is based on the product molecular weight 653.69.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
1 mM	1.53 mL	7.65 mL	15.30 mL
5 mM	0.31 mL	1.53 mL	3.06 mL
10 mM	0.15 mL	0.76 mL	1.53 mL
50 mM	0.03 mL	0.15 mL	0.31 mL

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References for MD13

References are publications that support the biological activity of the product.

Xiao Proteolysis targeting chimera (PROTAC) for macrophage migration inhibitory factor (MIF) has anti-proliferative activity in lung cancer cells. Angew.Chem.Int.Ed.Engl. 2021 PMID: 34018657