GNF 5

Name: GNF 5
Brand: Tocris Bioscience
SKU: 4908

Tocris Bioscience a Bio-Techne Brand

Catalog # 4908

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Key Product Details

Description:	Selective allosteric inhibitor of Bcr-Abl; analog of GNF 2 (Cat. No. 4399)
Chemical Name:	N-(2-Hydroxyethyl)-3-[6-[[4-(trifluoromethoxy)phenyl]amino]-4-pyrimidinyl]benzamide
Purity:	≥98% (HPLC)
Molecular Weight:	418.37

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Biological Activity for GNF 5

GNF 5 is a selective, non-ATP competitive allosteric inhibitor of Bcr-Abl (IC₅₀ = 220 nM for wild-type Abl). Binds the myristate-binding site of Abl. Acts in combination with nilotinib to inhibit T315I Bcr-Abl in vitro and in vivo. Analog of GNF 2 (Cat. No. 4399). GNF 5 decreases mitochondrial function in lung cancer cells.

Compound Libraries for GNF 5

GNF 5 is also offered as part of the Tocriscreen Antiviral Library. Find out more about compound libraries available from Tocris.

External Portal Information

Chemicalprobes.org is a portal that offers independent guidance on the selection and/or application of small molecules for research. The use of GNF 5 is reviewed on the chemical probes website.

Technical Data for GNF 5

M. Wt	418.37
Formula	C₂₀H₁₇F₃N₄O₃
Storage	Store at +4°C
Purity	≥98% (HPLC)
CAS Number	778277-15-9
PubChem ID	44129660
InChi Key	IIQUYGWWHIHOCF-UHFFFAOYSA-N
Smiles	O=C(NCCO)C1=CC(C2=NC=NC(NC3=CC=C(OC(F)(F)F)C=C3)=C2)=CC=C1

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility for GNF 5

	Solvent	Max Conc. mg/mL	Max Conc. mM
Solubility
Solubility	DMSO	41.84	100

Preparing Stock Solutions for GNF 5

The following data is based on the product molecular weight 418.37.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
1 mM	2.39 mL	11.95 mL	23.90 mL
5 mM	0.48 mL	2.39 mL	4.78 mL
10 mM	0.24 mL	1.20 mL	2.39 mL
50 mM	0.05 mL	0.24 mL	0.48 mL

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Reconstitution Calculator

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References for GNF 5

References are publications that support the biological activity of the product.

Luttman ABL allosteric inhibitors synergize with statins to enhance apoptosis of metastatic lung cancer cells. Cell Rep. 2021 PMID: 34706244
Iacob Allosteric interactions between the myristate- and ATP-site of the Abl kinase. PLoS One 2011 PMID: 21264348
Zhang Targeting Bcr-Abl by combining allosteric with ATP-binding-site inhibitors. Nature 2010 PMID: 20072125
Deng Expanding the diversity of allosteric Bcr-Abl inhibitors. J.Med.Chem. 2010 PMID: 20828158

Citations for GNF 5

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